Ribonucleic Acid-Mediated Control of Protein Translation Under Stress

Payea, Matthew J.; Dar, Showkat Ahmad; Malla, Sulochan; Maragkakis, Manolis

doi:10.1089/ars.2023.0233

Cited by 7 publications

(1 citation statement)

References 184 publications

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“…Cells respond to stress by triggering extensive transcriptome remodeling to restore cellular homeostasis (Galluzzi et al, 2018;Payea et al, 2023). Cellular stress responses have been under intense study as the capacity of cells to activate adaptive pathways deteriorates with age (Derisbourg et al, 2021;Haigis and Yankner, 2010;Maragkakis et al, 2022) and has been associated with many diseases, particularly age-related neurodegeneration (Beckman and Ames, 1998;Stadtman and Berlett, 1997).…”

Section: Introductionmentioning

confidence: 99%

Full-length direct RNA sequencing uncovers stress-granule dependent RNA decay upon cellular stress

Dar,

Malla,

Martinek

et al. 2023

Preprint

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Cells respond and adapt to endogenous and exogenous stressors by activating stress response pathways that restore cellular homeostasis. The capacity of cells to respond to stress declines with age and has been associated with disease. However, the changes in cytoplasmic RNA metabolism during the cellular stress response are poorly characterized. In this work, we employ end-to-end direct RNA sequencing with nanopores, following the ligation of unique 5' end adaptors, to assess the human transcriptome at single-molecule and -nucleotide resolution. To analyze this data, we developed NanopLen, a statistical tool to identify robust RNA length changes across conditions using linear mixed models. We find that upon cellular stress, RNAs are globally, subject to decay at their 5' end. Stress-induced decay is coupled to translation and ribosome occupancy and can be inhibited by restoring translation initiation or preventing ribosome run-off. In contrast to models of RNA decay under normal conditions, we show that stress-induced RNA decay is dependent on XRN1 but is independent of the poly(A) tail length. Surprisingly, we find that decaying RNAs persist in cells and are enriched in the stress granule transcriptome. Our results reveal the dynamics of post-transcriptional cell response that regulates the state of RNA upon cellular stress.

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Section: Introductionmentioning

confidence: 99%

Full-length direct RNA sequencing uncovers stress-granule dependent RNA decay upon cellular stress

Dar,

Malla,

Martinek

et al. 2023

Preprint

Self Cite

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Effects of polyphenols extracted from Keemun black tea on CYP450s activity and molecular mechanisms

Zuo,

Lv,

Ren

et al. 2024

Food Science & Nutrition

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Keemun black tea (KBT) is a luxurious traditional tea in China that has been commonly consumed because of its superior aroma and special taste. However, the risks remain unknown when KBT is used concomitantly with other drugs or food products. Therefore, we aimed to explore the effect of the tea polyphenols from KBT on the protein and mRNA levels of CYP450 and related mechanisms. The extraction of tea polyphenols from KBT and the content and component analysis of polyphenols were performed. A total of 24 female C57BL/6J mice were given tea polyphenols (0, 75, 150, 300 mg/kg) for 7 days, respectively. Liver tissues were collected 2 h after the last administration. The expression of Cyp3a11, Cyp1a2, Cyp2e1, Cyp2c37, and PXR mRNA was detected by real‐time PCR, and the expression of Cyp3a11, Cyp1a2, Cyp2e1, Cyp2c37, and PXR protein was detected by Western blotting. A transient co‐transfection reporter gene assay on HepG2 cells was also used to verify the role of PXR in regulating CYP3A4 expression. Our results showed that tea polyphenols from KBT significantly induced the expression of CYP 3A11 and PXR in general, inhibited the expression of Cyp1a2 and Cyp2e1 in general, and significantly inhibited the mRNA expression of Cyp2c37 but induced its protein expression. The reporter gene‐transfected cells demonstrated that tea polyphenols could enhance the PXR‐mediated transactivation of the CYP3A4 promoter via rifampicin‐induction. Meanwhile, tea polyphenols could significantly accelerate CYP3A11/3A4 expression by activating the PXR‐CYP3A4 pathway. In conclusion, KBT polyphenols could significantly affect the expression of various subtypes of the Cyp450 enzyme in mice livers via the PXR‐CYP450 pathway, suggesting that metabolism‐based interactions can occur when they are used in combination with medicines.

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Harnessing phytohormone dynamics to fortify crop resilience against drought: a comprehensive review

Peer

2024

Vegetos

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Ribonucleic Acid-Mediated Control of Protein Translation Under Stress

Cited by 7 publications

References 184 publications

Full-length direct RNA sequencing uncovers stress-granule dependent RNA decay upon cellular stress

Full-length direct RNA sequencing uncovers stress-granule dependent RNA decay upon cellular stress

Effects of polyphenols extracted from Keemun black tea on CYP450s activity and molecular mechanisms

Harnessing phytohormone dynamics to fortify crop resilience against drought: a comprehensive review

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