Ribonuclease-sensitive ribosomal vaccine of Pseudomonas aeruginosa

Gonggrijp, R.; Mullers, W. J. H. A.; Lemmens, P. J. M. R.; Boven, C P van

doi:10.1128/iai.27.1.204-210.1980

Cited by 15 publications

(12 citation statements)

References 18 publications

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“…In particular, the function of the ribosomes in the vaccines has been discussed, since contaminating cell envelope antigens were found in several of the ribosomal preparations (6,17,18). Previous studies of this laboratory (9) showed that a ribosomal vaccine of P. aeruginosa could be prepared which was sensitive to RNase, indicating that the ribosomal RNA was required for the protective activity of this vaccine. In addition, this vaccine could induce antibodies to the cell envelope component LPS (10).…”

Section: Resultsmentioning

confidence: 99%

“…Protective vaccines have been prepared from the ribosomes or ribosomal extracts of many different microorganisms, including Mycobacterium tuberculosis, Salmonella typhimurium, Streptococcus pneumoniae (5), and Pseudomonas aeruginosa (9,16). The diversity of the microorganisms from which protective ribosomal preparations could be prepared has raised the question of whether the ribosomes have a unique function in the induction of an immune response.…”

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confidence: 99%

“…MATERIALS AND METHODS Bacteria. P. aeruginosa serotype 3 and serotype 8 (strain RIV 76-5321 and 76-5309, respectively [9]) were used throughout for the preparation of vaccines and as challenging organisms.…”

mentioning

confidence: 99%

“…Vaccines. The ribosomal vaccine (fraction II) was obtained from a crude ribosomal preparation by molecular sieve chromatography on Sepharose Cl-2B as described previously (9). Four batches were pooled and further used as the ribosomal vaccine.…”

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confidence: 99%

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Serotype-Nonspecific Protection Induced by Ribonucleic Acid Isolated from the Ribosomal Vaccine of Pseudomonas aeruginosa

1981

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A ribosomal vaccine of Pseudomonas aeruginosa and a vaccine containing purified lipopolysaccharide (LPS) were compared with respect to their capacity to protect mice against a lethal challenge with P. aeruginosa. The route of vaccination appeared to be important for the protective activity of the ribosomal vaccine. Optimal protection was measured if both the immunizing and the challenge injection were given intraperitoneally. The ribosomal vaccine protected mice as early as 1 day after vaccination, and the protection lasted at least 6 days. LPS-specific antibodies were detectable 6 but not 2 days after vaccination. The ribosomal vaccine protected mice also against a heterologous serotype of Pseudomonas. Injection of purified LPS did not protect mice earlier than at day 3, and the protection induced by LPS was serotype specific. Ribonucleic acid (RNA) isolated from the ribosomal vaccine had the same protective properties as the ribosomes. RNA induced serotype-nonspecific protection as quickly as 1 day after injection, and the protection lasted at least 6 days. However, the capacity to induce antibodies to LPS was lost or reduced. It is concluded that the serotypenonspecific protection induced by RNA and the serotype-specific protection induced by LPS are due to different mechanisms. Experiments with combined

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Serotype-Nonspecific Protection Induced by Ribonucleic Acid Isolated from the Ribosomal Vaccine of Pseudomonas aeruginosa

1981

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“…Purification of RNA. RNA was extracted from purified ribosomes as described previously (1,11,12). The purified RNA contained less than 1% protein.…”

Section: Methodsmentioning

confidence: 99%

T-cell-independent macrophage activation in mice induced with rRNA from Listeria monocytogenes and dimethyldioctadecylammonium bromide

Bosch¹,

Kanis²,

Antonissen³

et al. 1986

Infect Immun

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Purified rRNA from Listeria monocytogenes or Pseudomonas aeruginosa injected in combination with dimethyldioctadecylammonium bromide (DDA), protects mice nonspecifically against a lethal challenge of various extra- and intracellular bacteria. In the present study vaccination of BALB/c as well as C57BL/Ka mice with listerial RNA-DDA resulted in activation of fixed-tissue macrophages, as measured by an enhanced in vivo L. monocytogenes killing in spleen and liver. Evidence was found that macrophage activation by vaccination with rRNA-DDA occurred by a T-cell-independent mechanism. Treatment of mice with cyclosporin A had no effect on the enhanced L. monocytogenes killing induced with RNA-DDA; in vitro exposure of RNA-DDA to spleen cell cultures did not give rise to any lymphocyte proliferation. No evidence could be found for a possible adjuvant activity for RNA-DDA in cellular responses; in fact, RNA-DDA had an inhibitory effect on lymphocyte proliferative responses to Listeria antigen and to concanavalin A.

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Protective activities of ribosomal ribonucleic acid and lipopolysaccharide of Pseudomonas aeruginosa: a comparative study

Gonggrijp

Mullers

Boven

1983

Antonie van Leeuwenhoek

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Ribosomal ribonucleic acid (RNA) and lipopolysaccharide (LPS) from P. aeruginosa were compared with respect to their protective activities in mice against an infection with P. aeruginosa. This study is concentrated on the protective activity of RNA. RNA isolated from purified ribosomes did not contain LPS as determined with the Limulus test. Injection of RNA with the adjuvant dimethyldioctadecylammonium bromide (DDA) protected mice against P. aeruginosa without inducing LPS-specific antibodies. C3H/HeJ mice which are relatively insensitive to the protective activity of LPS could be protected with RNA. The protective activities of RNA and LPS from a mutant strain of P. aeruginosa, PAC 605, containing defective lipopolysaccharide, were compared with the protective activities of RNA and LPS from the parent strain, PAC IR. The protective activity of LPS from PAC 605 was 1000 fold lower than the protective activity of LPS from PAC IR. RNA preparations of both strains induced similar percentages of survival. The protective activity of ribosomal RNA from P. aeruginosa was nonspecific since mice were also protected against a heterologous serotype of P. aeruginosa and against Escherichia coli. RNA from ribosomes of P. aeruginosa, E. coli and the non-lipopolysaccharide containing Saccharomyces cerevisiae had similar protective activities. No protection was obtained with the ribonucleic acid from the E. coli phage MS2. It is concluded that ribosomal RNA has protective activities distinct from those of LPS.

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Ribonuclease-sensitive ribosomal vaccine of Pseudomonas aeruginosa

Cited by 15 publications

References 18 publications

Serotype-Nonspecific Protection Induced by Ribonucleic Acid Isolated from the Ribosomal Vaccine of Pseudomonas aeruginosa

Serotype-Nonspecific Protection Induced by Ribonucleic Acid Isolated from the Ribosomal Vaccine of Pseudomonas aeruginosa

T-cell-independent macrophage activation in mice induced with rRNA from Listeria monocytogenes and dimethyldioctadecylammonium bromide

Protective activities of ribosomal ribonucleic acid and lipopolysaccharide of Pseudomonas aeruginosa: a comparative study

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