Ribonuclease of bovine milk: Purification and properties

Bingham, Elizabeth W.; Zittle, Charles A.

doi:10.1016/0003-9861(64)90182-1

Cited by 37 publications

(9 citation statements)

References 19 publications

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“…Amino acid analysis, electrophoresis and immunological studies showed that milk RNase is identical to pancreatic RNase (Bingham & Zittle, 1964). It was presumed that the RNase in milk originated in the pancreas and was absorbed through the intestinal wall into the blood, from which it enters milk.…”

Section: Ribonucleasementioning

confidence: 98%

“…The RNase in bovine milk was further purified from acid whey by Bingham and Zittle (1964), using the same general procedure but with elution from Amberlite IRC-50 using a NaCl gradient, which resolved two isoenzymes, A and B, at a ratio of about 4:1, as for pancreatic RNase. Amino acid analysis, electrophoresis and immunological studies showed that milk RNase is identical to pancreatic RNase (Bingham & Zittle, 1964).…”

Section: Ribonucleasementioning

confidence: 99%

“…RNase A and B were isolated from bovine milk by Bingham and Kalan (1967) essentially by a scaled-up version of the procedure of Bingham and Zittle (1964) and including a gel permeation step. Two other isoenzymes, C and D, were demonstrated but not purified.…”

Section: Ribonucleasementioning

confidence: 99%

See 2 more Smart Citations

Indigenous enzymes in milk: Overview and historical aspects—Part 2

Fox

Kelly

2006

International Dairy Journal

101

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Between 1924 and about 1970, many indigenous enzymes were identified in milk. These were important as indicators of the adequate pasteurisation of milk (alkaline phosphatase, g-glutamyl transferase) or of mastitis (N-acetylglucosaminidase, acid phosphatase) and some were considered to be important for the stability of milk (superoxidase dismutase, sulphydryl oxidase). Human and equine milk both contain a very high level of lysozyme, which is considered to have a significant protective effect on the neonate. Progress on the isolation and characterisation of these seven enzymes first isolated in the period 1925-1970, as well as ribonuclease, aldolase and glutathione peroxidase, from the milk of the cow and other species and their significance in milk and dairy products is reviewed in this article. r

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Section: Ribonucleasementioning

confidence: 98%

Section: Ribonucleasementioning

confidence: 99%

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Indigenous enzymes in milk: Overview and historical aspects—Part 2

Fox

Kelly

2006

International Dairy Journal

101

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“…Ribonucleases cleave RNA into smaller components; five ribonucleases, A, B, C, D and II-1, have been detected in milk (Bingham and Zittle 1964). Meyer et al (1987) reported that colostrum contains 10 to 15 times more ribonuclease II-1 than milk and three times more total ribonuclease activity.…”

Section: Other Enzymesmentioning

confidence: 99%

Composition and properties of bovine colostrum: a review

McGrath

Fox

McSweeney

et al. 2015

Dairy Sci. & Technol.

302

263

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Colostrum is the initial milk secreted by mammals following parturition, the composition and physicochemical properties of which are highly dynamic and variable. The composition and physicochemical properties of colostrum during the initial postpartum period has not been systematically reviewed for many years, although the topic remains of interest both to milk producers and processors. In this article, the current understanding of the composition of colostrum, i.e. carbohydrates, proteins, growth factors, enzymes, enzyme inhibitors, nucleotides and nucleosides, cytokines, fats, vitamins and minerals, is reviewed. In addition, the physicochemical properties, i.e. pH and buffering capacity, colour, density and specific gravity, osmotic pressure, somatic cell count, properties of casein micelles, ethanol stability and rennet coagulation properties are discussed, as well as the effects of heat-treating colostrum.

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“…In bovines, besides pancreatic RNAase A, other alkaline RNAases have been studied and characterized from spleen [49], milk [50] and aorta [51]. However, it would appear as if these enzymes, and the one from milk in a special way, are quite similar to RNAase A.…”

Section: Discussionmentioning

confidence: 99%

Bull Semen Ribonucleases. 1. Purification and Physico-Chemical Properties of the Major Component

Flobidi²,

et al. 1972

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A high ribonuclease activity has been detected in bull seminal plasma; two major fractions (RNAase BS-1 and RNAase BS-2) have been identified, which are responsible for such activity and one of the two, RNAase BS-I, has been purified and crystallized.It has a molecular weight of 29000, an isoelectric point a t pH 10.3 and A:'&, a t 278 nm is 4.65, The amino acid composition has been determined, its main features being a high content of basic residues, the absence of tryptophan and cysteine, and the presence of 18 half-cystine residues.The enzyme is produced by the seminal vesicles, and occurs in seminal plasma as a free, soluble component.For some years we have been studying the ribonuclease activity which we have found to occur in high concentration in the seminal plasma of the bull, with general properties similar to those of bovine pancreatic ribonuclease. We have seen that such activity is the result of a t least two enzymes and our research has been focused on the major component. We wish to give here a full report of the purification procedure of this enzyme (RNAase BS-I), with its amino acid composition and the relevant physicochemical properties, while the following paper [l] will deal with the mechanism of action.Partial results of our research have already been published [2-61.

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Ribonuclease of bovine milk: Purification and properties

Cited by 37 publications

References 19 publications

Indigenous enzymes in milk: Overview and historical aspects—Part 2

Indigenous enzymes in milk: Overview and historical aspects—Part 2

Composition and properties of bovine colostrum: a review

Bull Semen Ribonucleases. 1. Purification and Physico-Chemical Properties of the Major Component

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