Ribonuclease binase inhibits primary tumor growth and metastases via apoptosis induction in tumor cells

Миронова, Н. Л.; Petrushanko, Irina Yu.; Patutina, Olga; Sen’kova, Aleksandra V.; Simonenko, O V; Mitkevich, Vladimir A.; Markov, Oleg V.; Zenkova, Marina A.; Makarov, Alexander А.

doi:10.4161/cc.25164

Cited by 38 publications

(38 citation statements)

References 40 publications

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“…TNF-α also triggers apoptosis, resulting in initiating extrinsic pathway with cell death receptor by activating caspase-8 [33]. We found similar results with NF-KB for TNF-α.…”

Section: Discussionsupporting

confidence: 78%

Counteraction of Apoptotic and Inflammatory Effects of Adriamycin in the Liver Cell Culture by Clinopitolite

Yapışlar

Taşkın

Ozdas

et al. 2015

Biol Trace Elem Res

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Growing evidence has been reported on adriamycin (ADR) hepatotoxicity in literature. Hepatotoxicity caused by the use of drugs has a serious undesirable effect in the cure of cancer patients that needs to be eliminated. The exact mechanism of ADR on non-cancerous tissue still remains to be a mystery. The zeolite (clinoptilolite) minerals form a complex group of aluminosilicates that often occur as accessory minerals in intermediate and basic rocks. In light of this information, we investigated the possible anti-inflammatory and anti-apoptotic effects of clinoptilolite in ADR that is inducing the toxicity in primary liver cell culture. Primary liver cell culture from rat was used in the study. We had three experiment groups including the following: (1) cells treated only with 50 μM ADR for 24 h, (2) cells treated with the 50 μM ADR for 24 h and then treated with 10(-4) M zeolite for 1 h, and (3) cells were incubated with 50 μM ADR for 24 h and then incubated with 10(-4) M zeolite for 24 h to test its long-term effects. After that, western blotting was performed in order to evaluate protein expression levels of several inflammation markers including IL-1β, tumor necrosis factor (TNF)-α, and nuclear factor kappa B (NF-κB), and immunohistochemistry was carried out to detect apoptosis in liver cell culture. Also, TdT-dUTP Terminal Nick-End Labeling (TUNEL) method was used for detecting apoptosis. We found elevated levels of inflammatory protein and apoptotic markers in ADR-administered cells (p < 0.05). Inflammatory and apoptotic markers decreased significantly after treated with zeolite (p < 0.05). The present study was pointed out that ADR causes hepatotoxicity via apoptosis and/or inflammation processes resulting from initiator NF-κB and TNF which causes proinflammatory mediators such as IL-1β. Elevation of inflammation might give rise to trigger apoptosis. Clinoptilolite counteracted the apoptosis and inflammation induced by ADR arising from the decrease in NF-κB, TNF-α, and IL-1β protein levels.

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“…TNF-α also triggers apoptosis, resulting in initiating extrinsic pathway with cell death receptor by activating caspase-8 [33]. We found similar results with NF-KB for TNF-α.…”

Section: Discussionsupporting

confidence: 78%

Counteraction of Apoptotic and Inflammatory Effects of Adriamycin in the Liver Cell Culture by Clinopitolite

Yapışlar

Taşkın

Ozdas

et al. 2015

Biol Trace Elem Res

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“…Ribonucleases of bacterial origin are perspective tools for the development of new pharmaceutical and biotechnological agents which have a potential as antitumor (Mironova et al, 2013;Mitkevich et al, 2014) and antiviral (Shah Mahmud and Ilinskaya, 2013) drugs. In order to estimate the biodiversity of RNase-secreting strains, to conduct comparative studies and to find new RNase producers we have performed database searches for homologues of barnase.…”

Section: Resultsmentioning

confidence: 99%

“…B. intermedius RNase referred to as binase is as well studied as barnase. Its antitumor and antiviral properties are documented (Mironova et al, 2013;Mitkevich et al, 2014;Shah Mahmud and Ilinskaya, 2013;Ulyanova et al, 2011) while the strain itself is an undescribed taxon which is Phylogenetic distribution of extracellular guanyl-preferring ribonucleases renews taxonomic status of two Bacillus strains † (Received January 20, 2016;Accepted February 18, 2016; J-STAGE Advance publication date: July 4, 2016) Vera Ulyanova, 1,2,* Raihan Shah Mahmud, 1 Elena Dudkina, 1 Valentina Vershinina, absent in the List of Prokaryotic Names with Standing in Nomenclature (LPSN) (Parte, 2014). Primary structures of B. intermedius and B. pumilus RNases are identical and differ from that of B. thuringiensis by one amino acid only (Dement'ev et al, 1993;Shul'ga et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Phylogenetic distribution of extracellular guanyl-preferring ribonucleases renews taxonomic status of two <i>Bacillus</i> strains

Ulyanova

Mahmud

Dudkina

et al. 2016

J. Gen. Appl. Microbiol.

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† The GenBank accession number for the 16S rRNA gene sequence of Bacillus altitudinis B-388 is JX129389, for the rpoB gene sequence of Bacillus altitudinis B-388 is JX129391, for the 16S rRNA gene sequence of Bacillus pumilus 7P is JX129390 and for the rpoB gene sequence of Bacillus pumilus 7P is JX129392 correspondingly.None of the authors of this manuscript has any financial or personal relationship with other people or organizations that could inappropriately influence their work. IntroductionBarnase, an extracellular alkaline guanyl-preferring ribonuclease first isolated from Bacillus amyloliquefaciens (Hartley and Rogerson, 1972), was shown to possess a wide range of biological activities and is of special interest for research and practical applications in biotechnology and medicine (Kempe et al., 2014;Spång et al., 2012;Sreenivasan et al., 2013;Ulyanova et al., 2011). Ribonucleases similar to barnase (UniProtKB/Swiss-Prot accession number P00648) were purified from B. intermedius 7P (P00649), B. circulans BCF-256 (P35078), B. pumilus KMM-62 (P48068), B. coagulans BCF-247 (P37203), and B. thuringiensis var. subtoxicus B-388 (Q9R5D7, GenBank acc No. AAB28984.1). B. intermedius RNase referred to as binase is as well studied as barnase. Its antitumor and antiviral properties are documented (Mironova et al., 2013;Mitkevich et al., 2014;Shah Mahmud and Ilinskaya, 2013;Ulyanova et al., 2011) while the strain itself is an undescribed taxon which is The potential of microbial ribonucleases as promising antitumor and antiviral agents, determines today's directions of their study. One direction is connected with biodiversity of RNases. We have analyzed completed and drafted Bacillus genomes deposited in GenBank for the presence of coding regions similar to the gene of an extracellular guanyl-preferring RNase of Bacillus amyloliquefaciens (barnase). Orthologues of the barnase gene were detected in 9 species out of 83. All of these belong to "B. subtilis" group within the genus. B. subtilis itself, as well as some other species within this group, lack such types of RNases. RNases similar to barnase were also found in species of "B. cereus" group as a part of plasmid-encoded S-layer toxins. It was also found that taxonomic states of culture collection strains, which were initially described based on a limited set of phenotypic characteristics, can be misleading and need to be confirmed. Using several approaches such as matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS), sequencing of genes for 16S ribosomal RNA and RNA polymerase subunit beta followed by reconstruction of phylogenetic trees, we have re-identified two RNase-secreting Bacillus strains: B. thuringiensis B-388 which should be assigned as B. altitudinis B388 and B. intermedius 7P which should be renamed as B. pumilus 7P. Therefore, small secreted guanyl-preferring RNases are the feature of "B. subtilis" group only, which is characterized by distinctive lifestyle and adaptation strategies to environment.

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“…Wnioski są zgodne z dotychczasowymi danymi na temat rodziny rybonukleaz, do których należy RNaza 7. Rybonukleazy katalizują rozkład kwasów rybonukleinowych i są znane z aktywności przeciwnowotworowej [45,46].…”

Section: Peptydy Przeciwdrobnoustrojowe W Nowotworach Skóryunclassified

The role of antimicrobial peptides in skin tumorigenesis

Marcinkiewicz¹,

Majewski²

2016

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Ribonuclease binase inhibits primary tumor growth and metastases via apoptosis induction in tumor cells

Cited by 38 publications

References 40 publications

Counteraction of Apoptotic and Inflammatory Effects of Adriamycin in the Liver Cell Culture by Clinopitolite

Counteraction of Apoptotic and Inflammatory Effects of Adriamycin in the Liver Cell Culture by Clinopitolite

Phylogenetic distribution of extracellular guanyl-preferring ribonucleases renews taxonomic status of two <i>Bacillus</i> strains

The role of antimicrobial peptides in skin tumorigenesis

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