Ribonuclease activity of Glycine max and Glycine soja sprouts as a marker adaptation to copper sulphate and zinc sulphate toxicity

Lavrent’yeva, S. I.; Ivachenko, Lubov Yegorovna; Golokhvast, Kirill S.; Nawaz, Muhammad Amjad

doi:10.1016/j.bse.2019.01.007

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…The link between copper and these systems highlights its importance in maintaining health, with recommended doses of 1.5-3.0 mg in adults and 1 mg in children [52]. High concentrations of copper [64] act as an enzyme inhibitor and limit the activity of alkaline phosphatase [65], catalase [22], xanthine oxidase [66] and ribonuclease [67,68].…”

Section: Introductionmentioning

confidence: 99%

<i>Drosophila melanogaster </i>as a Bioindicator in Comparative Copper and Lead Toxicology: Exploring the Health Implications of Heavy Metal Exposure

Sarac,

Onisan,

Petrescu

et al. 2024

Preprint

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Drosophila melanogaster serves as a important research model both for genetic investigations and for the study of metal toxicity, facilitating the elucidation of physiological mechanisms comparable to those of human organisms. In our research, we evaluated the toxicity effect resulting from exposure to various concentrations of lead and copper on the prolificity rates throughout the life cycle (egg-adult) of four genotypes of Drosophila melanogaster: wild-type (control), white, brown, and white-vestigial. During our study, the prolificity rates were examined across three repetitions under the impact of exposure to concentrations of 0.50, 1.00, 2.00, and 4.00 mM of copper (CuSO4) and lead (Pb(C2H3O2)2). Prolificity rates throughout the life cycle exhibited variations as a direct consequence of genetic factors, the concentration of exposure, and the specific type of metal, either copper or lead. The mutant white-vestigial genotype revealed an IC50 concentration for prolificity inhibition at lower doses of 2.00 mM for copper and 4.00 mM for lead, in contrast to the control genotype (wild-type), which exhibited an inhibition concentration rate >IC50 of 4.00 mM only in the case of copper. Our results concluded that the (i) dose influences the prolificity rate in a directly proportional manner, (ii) comparative analyses between copper and lead revealed that copper displayed toxicity across all genotypes within the concentration range of 0.50 mM to 2.00 mM. In contrast, lead exhibited toxicity within the concentration range of 1.00 mM to 4.00 mM, highlighting a (iii) more acute toxicity characteristic in the case of copper. Thus, the results of this research reflect the importance of using Drosophila melanogaster as a genetic model in the comparative study of the interaction between genetic factors and the toxicity of metals, offering significant insights into monitoring their impact and defining the maximum permissible doses on organisms.

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Section: Introductionmentioning

confidence: 99%

<i>Drosophila melanogaster </i>as a Bioindicator in Comparative Copper and Lead Toxicology: Exploring the Health Implications of Heavy Metal Exposure

Sarac,

Onisan,

Petrescu

et al. 2024

Preprint

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“…The amount of copper in the blood of healthy people is 1.23 ± 0.16 mg/L [18]. High concentrations of copper act as an enzyme inhibitor and limit the metabolic activity [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Application of the Drosophila melanogaster Research Model to Evaluate the Toxicity Levels between Lead and Copper

Ioan,

Irina,

Emilian

et al. 2024

Applied Sciences

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Applications of the Drosophila melanogaster (D.m.) research model have an important means both for genetic investigations and for the study of metal toxicity, because D.m. has physiological mechanisms comparable to those in human organisms. In this research, the toxic effect of lead (Pb2+) and copper (Cu2+) on four D.m. genotypes—the wild genotype (Oregon-R, used as control) and three mutant genotypes (white, brown, and white-vestigial)—was compared. Five replicates were made to observe the development progress of monitorized genotypes exposed to five different concentrations 0, 0.50, 0.75, 1.00, and 2.00 mM of copper (CuSO4) and lead Pb(C2H3O2)2. Proliferation rates of larvae, pupae, and adults depend on genetic factors, metals used (copper or lead), and their concentrations. The white-vestigial mutant genotype showed the greatest sensitivity at IC50 concentration (inhibition of proliferation of more than 50% compared with the control sample) at doses of 1.00 mM for Cu2+ and 2.00 mM for Pb2+. In contrast, the control genotype (Oregon-R) showed only an inhibition IC50 concentration of 2.00 mM for Cu2+. The white-vestigial mutant genotype showed the greatest sensitivity at IC50 concentration (inhibition of proliferation of more than 50% compared with the control sample) at doses of 1.00 mM for Cu2+ and 2.00 mM for Pb2+. In contrast, the control genotype (Oregon-R) showed an inhibition at the IC50 concentration of 2.00 mM for Cu2+. The results conclude that (i) the dose influences the prolificacy rate in a directly proportional way, (ii) the comparative analyses between Cu2+ and Pb2+ revealed a more acute effect of Cu2+, and (iii) differentiated prolificacy values according to genotypes were recorded. Those reflect the importance of using D.m. as a research model in the comparative studies of the interactions between genetic factors and metal toxicity. Also, this study provides significant information on non-toxic maximum doses for organisms.

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Biochemical adaptation of wild and cultivated soybean against toxicity of lead salts

Lavrent’yeva

Чернышук

Martinenko

et al. 2020

Environmental Toxicology and Pharmacology

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Ribonuclease activity of Glycine max and Glycine soja sprouts as a marker adaptation to copper sulphate and zinc sulphate toxicity

Cited by 5 publications

References 18 publications

<i>Drosophila melanogaster </i>as a Bioindicator in Comparative Copper and Lead Toxicology: Exploring the Health Implications of Heavy Metal Exposure

<i>Drosophila melanogaster </i>as a Bioindicator in Comparative Copper and Lead Toxicology: Exploring the Health Implications of Heavy Metal Exposure

Application of the Drosophila melanogaster Research Model to Evaluate the Toxicity Levels between Lead and Copper

Biochemical adaptation of wild and cultivated soybean against toxicity of lead salts

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