The effect of continuous-flow microwave heating of milk on the stability of vitamins B 1 and B 2 was determined by ion-pair reverse-phase high-performance liquid chromatography. Results were compared with those obtained using a conventional process having the same heating, holding and cooling phases. When milk was heated in a continuous microwave heating system, at 90 °C without a holding phase, no vitamin B 1 and vitamin B 2 losses were observed. However, when the holding time was raised to 30 s or 60 s, while the content of vitamin B 2 was not modified, the content of vitamin B 1 was lowered (3% and 5%, respectively). Analogous results were obtained when the milk was submitted to a similar heating process using a conventional system. These results indicate that continuous-flow microwave heating of milk at high temperature does not offer any advantage with respect to the vitamin B 1 and B 2 retention compared with a conventional heating process having the same heating, holding and cooling times. vitamin B 1 / vitamin B 2 / milk / microwave heating