Riboflavin of Sow's Milk

Davis, Virginia E.; MacVicar, Robert; Ross, C. B.; Whitehair, C. K.; Heidebrecht, A. A.; Braude, R.; Coates, Michael I.; Henry, K. M.; Kon, S. K.; Thompson, S. Y.; Wilby, Frances

doi:10.1038/165522a0

Cited by 11 publications

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“…A microbiological assay may, however, have considerable advantages over a nonbiological technique, as is shown by the measurement of riboflavin in sow's milk (see Davis et al 1950). Good agreement was reached between the microbiological and chick techniques, whereas a chemical method well-tried for cow's milk gave much lower results; further investigation revealed the presence in the sow's milk of riboflavin in a form not detectable by the chemical procedure used.…”

Section: '0 2'3-4'3mentioning

confidence: 99%

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The Use of Chicks for the Biological Assay of Members of the Vitamin B Complex

et al. 1952

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Vitamin metabolism of the fowl. I 754. The shell gland has three-quarters of its riboflavin present as dinucleotide, the 5. It is suggested that the magnum is the tissue involved in the day-to-day rest being equally divided between the free form and the mononucleotide.mechanism affecting the amount of riboflavin in the egg.The author thanks Dr A. W. Greenwood, the Director of this Centre, for his advice and criticism, and Miss D. R. Mitchell for assistance both in the care of the birds and in the analyses. M. E. COATES AND OTHERS 1952which, mainly because of their speed and simplicity of operation, are increasingly being used for routine determinations of the vitamins. Since they are applied in particular at this laboratory to experiments on the synthesis of vitamins by the micro-organisms of the alimentary tract, it was thought advisable to examine certain gut-content materials by the chick method.The following twelve substances were tested for one or more of the vitamins listed; (9) Dried spinach-beet ; a mixture of spinach-beet leaves freshly picked, freed from stalk, immediately dried in a current of warm air at about 4 5 O and milled before use.(10) Dried rat faeces, collected from normal stock-colony rats and dried at about 70°, then milled finely.(I I) Dried whole rumen contents, kindly supplied by our colleague Dr J. W. G.Porter, collected from a fistulated cow over several weeks, spread on stainless steel trays, dried in a current of warm air at about 45', then milled and the whole sample remixed mechanically before use.(12) Fish solubles, kindly supplied by Dr J. A. Lovern of the Torry Research Station, Aberdeen; a concentrate of a watery extract obtained in the course of manufacture of herring oil by the ' cook-and-press' method. StandardsFor the work with chicks commercially pure samples of the vitamins were used throughout, since the quantities involved were large. For microbiological tests the Medical Research Council Provisional Standards were used when available ; synthetic pteroylglutamic acid (Lederle Laboratories Inc.) was used as folic-acid standard in both methods. The two sets of standards were compared microbiologically and corrections were made when necessary, the results being expressed in terms of microbiological standards. Most of the materials were bulky and of low potency, so that it was sometimes necessary to replace as much as 20 yo of the diet by the test sample to get a measurable response. The basic components of the diet were then adjusted to preserve the nutritive value of the basal diet as far as possible unchanged. Where the sample, such as dried faeces or flour, consisted mainly of fibrous or carbohydrate material, it was added at the expense of the dextrin. If it contained an appreciable amount of protein, as in dried milk, some of the casein was omitted from the basal diet. Certain materials for which a chick assay was required were of too low potency to give a measurable response even when forming 20 % of the diet. Such samples were fortified before testing by the addi...

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Section: '0 2'3-4'3mentioning

confidence: 99%

“…(7) Dried skim milk; a commercial sample of good quality. (8) Sow's milk; a pooled, freeze-dried sample of milk from several sows, prepared as described by Davis, MacVicar, Ross, Whitehair, Heidebrecht, Braude, Coates, Henry, Kon, Thompson & Wilby (1950).…”

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The Use of Chicks for the Biological Assay of Members of the Vitamin B Complex

et al. 1952

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“…The conclusion was drawn (Davis et al, 1950) that riboflavin is present in sows' milk at least partly in a form different from that in cows' milk.…”

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