Riboflavin as a promising antimicrobial agent? A multi-perspective review

Farah, Nuratiqah; Chin, Voon Kin; Chong, Pei Pei; Lim, Wai Feng; Lim, Jonathan Chee Woei; Basir, Rusliza; Chang, Sui Kiat; Lee, Tze Yan

doi:10.1016/j.crmicr.2022.100111

Cited by 39 publications

(30 citation statements)

References 143 publications

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“…It plays a key role in the homeostasis of the human body ( Dey and Bishayi, 2016 ), regulation of multiple metabolic pathways driven by redox reactions ( Powers, 2003 ) or the metabolism of different vitamins (such as folic acid, niacin, pyridoxine, and cobalamin) through the action of FMN and FAD ( Pinto and Rivlin, 2013 ). Besides its important role in maintaining human health, recently the antimicrobial activity of RF against parasites, fungi, viruses and bacteria has been demonstrated ( Farah et al, 2022 ). Therefore, in situ biofortification of fermented foods through the use of LAB that overproduce vitamin B 2 and dextran-type EPS, is a promising strategy to strengthen the health of consumers and address different nutritional deficiencies.…”

Section: Introductionmentioning

confidence: 99%

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

et al. 2023

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Fermentative processes by lactic acid bacteria can produce metabolites of interest to the health and food industries. Two examples are the production of B-group vitamins, and of prebiotic and immunomodulatory dextran-type exopolysaccharides. In this study, three riboflavin- and dextran-producing Weissella cibaria strains (BAL3C-5, BAL3C-7 and BAL3C-22) were used to develop a new method for selection and isolation of spontaneous riboflavin-overproducing W. cibaria mutants. This method was based on the selection of strains resistant to roseoflavin. The DNA sequencing of the FMN riboswitch of bacterial cell populations treated with various roseoflavin concentrations, revealed the existence of at least 10 spontaneous and random point mutations at this location. Folding and analysis of the mutated FMN riboswitches with the RNA fold program predicted that these mutations could result in a deregulation of the rib operon expression. When the roseoflavin-treated cultures were plated on medium supporting dextran synthesis, the most promising mutants were identified by the yellow color of their mucous colonies, exhibiting a ropy phenotype. After their isolation and recovery in liquid medium, the evaluation of their riboflavin production revealed that the mutant strains synthesized a wide range of riboflavin levels (from 0.80 to 6.50 mg/L) above the wild-type level (0.15 mg/L). Thus, this was a reliable method to select spontaneous riboflavin-overproducing and dextran-producing strains of W. cibaria. This species has not yet been used as a starter or adjunct culture, but this study reinforces the potential that it has for the food and health industry for the production of functional foods or as a probiotic. Furthermore, analysis of the influence of FMN present in the growth medium, on rib mRNA and riboflavin levels, revealed which mutant strains produce riboflavin without flavin regulation. Moreover, the BAL3C-5 C120T mutant was identified as the highest riboflavin-overproducer. Determination of its chromosomal DNA sequence and that of BAL3C-5, revealed a total identity between the 2 strains except for the C120T mutation at the FMN riboswitch. To our knowledge, this work is the first demonstration that only a single alteration in the genome of a lactic acid bacteria is required for a riboflavin-overproducing phenotype.

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Section: Introductionmentioning

confidence: 99%

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

et al. 2023

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“…With this, we aimed to eliminate cross-linkings to human-similar proteins since metabolic pathways or enzymes that are missing from the human host have been an important resource of druggable targets against infectious diseases 53 . From the 37 genes that our model predicted to be essential (with FBA and MOMA) contradicting the experimental results, 17 were found to be non-homologous (see Supplementary File S7 55 . Lastly, the phosphogluconate dehydratase catalyzes the dehydration of 6-Phospho-D-gluconate to KDPG, the precursor of pyruvate and 3-Phospho-D-glycerate 56 .…”

Section: Nantia Leonidoumentioning

confidence: 52%

Exploring the metabolic profiling ofA. baumanniifor antimicrobial development using genome-scale modelling

Leonidou,

Xia,

Friedrich

et al. 2023

Preprint

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With the emergence of multidrug-resistant bacteria, the World Health Organization published a catalog of microorganisms in 2017 for which new antibiotics are urgently needed. Within this list, the carbapenem-resistant pathogenAcinetobacter baumannii, belonging to the ESKAPE group, has been granted the "critical" status. Over the years, such isolates have been detected within healthcare units, posing a global threat to upcoming pandemics. One way to facilitate a systemic view of bacterial metabolism and allow the development of new therapeutics based on environmental and genetic alterations is to apply constraint-based modeling on metabolic networks. We developed a versatile workflow to build high-quality and simulation-ready genome-scale metabolic models. We applied our workflow to create a novel metabolic model forA. baumanniiand validated its predictive capabilities using experimental nutrient utilization and gene essentiality data. Our analysis showed that our modeliACB23LX could recapitulate cellular metabolic phenotypes observed during in vitro experiments with an accuracy of over 80%, while positive biomass production rates were observed in growth media relevant toA. baumannii. Additionally, we identified putative essential genes with no human counterparts, which could serve as novel antibiotic candidates for the development of future antimicrobial strategies. Finally, we have assembled the first curated collection of available reconstructions for distinctA. baumanniistrains and analyzed their growth characteristics. The presented models herein are in a standardized and well-curated format, facilitating their usability, while they can be used to guide the reconstruction of multi-strain networks. Ultimately, they serve as a knowledge base for reliable predictions under various perturbations and the development of effective drugs.

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“…Of note, in the absence of an external light source, VB 2 SMRs demonstrated certain antibacterial activity derived from their own bioactivity against both MRSA and E. coli O157:H7, which is consistent with the results of previous studies. 54,55 Figures 4e,f and S25 illustrate that such inherent antibacterial activity exhibits a distinct dose-dependent relationship with the dosage of VB 2 SMRs, which was particularly evident in the high VB 2 SMRs dosage group. Live/dead staining assay presents a similar trend to the plate counting method.…”

Section: ■ Results and Discussionmentioning

confidence: 94%

Reassembled One-Dimensional VB₂ Submicrorods with Enhanced Photosensitivity and H₂O₂ Supply for Efficient Antibacterial Therapy

Feng,

Yang,

Wang

et al. 2023

ACS Sustainable Chem. Eng.

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Recent advances in photocatalytic micro-nanoarchitecture have enlightened emerging photosensitizer-based photodynamic bactericidal therapies, which have been fruitful in addressing the thorny issue of multidrug-resistant bacterial infections. Nevertheless, the clinical feasibility of current photodynamic sterilization has been constrained by the biosafety of the photosensitizer and the maintenance of a relatively mild physiological microenvironment during treatment. Herein, we assemble a rodlike monocrystal on the sub-microscale from bioactive vitamin B 2 (VB 2 ) through a precise control strategy. The internal VB 2 single molecules form a onedimensional submicrostructure driven by π−π stacking interactions and hydrogen bonds. As expected, the dimensional submicronization tuning strategy ameliorates the aqueous solubility and dispersion stability of ordinary VB 2 crystals while endowing them with enhanced superoxide radical yield and H 2 O 2 supply under illumination. Photodynamic therapy (PDT) based on VB 2 submicrorods demonstrated remarkable in vitro killing effects against Gram-positive and Gram-negative bacteria by mediating ROS bursts. Furthermore, the superior biocompatibility of VB 2 submicrorods enables them to accelerate the closure of exposed lesions during PDT of multidrug-resistant bacterial infections without inducing perceptible side effects. This work aims to pursue photosensitizers with high biosafety, efficiency, and practical feasibility and focuses on developing biologically active VB 2 as an environmentally friendly and cost-effective multifunctional photodynamic bactericide.

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Riboflavin as a promising antimicrobial agent? A multi-perspective review

Cited by 39 publications

References 143 publications

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

Exploring the metabolic profiling ofA. baumanniifor antimicrobial development using genome-scale modelling

Reassembled One-Dimensional VB₂ Submicrorods with Enhanced Photosensitivity and H₂O₂ Supply for Efficient Antibacterial Therapy

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Riboflavin as a promising antimicrobial agent? A multi-perspective review

Cited by 39 publications

References 143 publications

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

Exploring the metabolic profiling ofA. baumanniifor antimicrobial development using genome-scale modelling

Reassembled One-Dimensional VB2 Submicrorods with Enhanced Photosensitivity and H2O2 Supply for Efficient Antibacterial Therapy

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About

Reassembled One-Dimensional VB₂ Submicrorods with Enhanced Photosensitivity and H₂O₂ Supply for Efficient Antibacterial Therapy