Rhythmic potassium transport regulates the circadian clock in human red blood cells

Henslee, Erin A.; Crosby, Priya; Kitcatt, Stephen J.; Parry, Jack S. W.; Bernardini, Andrea; Abdallat, Rula G.; Braun, Gabriella; Fatoyinbo, Henry O.; Harrison, Esther J.; Edgar, Rachel S.; Hoettges, Kai F.; Reddy, Akhilesh B.; Jabr, Rita I.; Schantz, Malcolm von; O’Neill, John S.; Labeed, Fatima H.

doi:10.1038/s41467-017-02161-4

Cited by 71 publications

(116 citation statements)

References 45 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…In light of previous observations 9, 42 , we hypothesised that transmembrane ion flux was responsible for this isovolumetric compensatory mechanism. We focused on intracellular metal ions, the most abundant osmotically active species in cells, and analysed the intracellular ionic composition of primary fibroblasts across three circadian cycles by inductively-coupled plasma mass spectrometry (ICP-MS).…”

Section: Antiphasic Oscillations In Soluble Protein and Intracellularmentioning

confidence: 69%

Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology

Stangherlin

Wong

Barbiero

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Between 6-20% of the cellular proteome is under circadian control to tune cell function with cycles of environmental change. For cell viability, and to maintain volume within narrow limits, the osmotic pressure exerted by changes in the soluble proteome must be compensated. The mechanisms and consequences underlying compensation are not known. Here, we show in cultured mammalian cells and in vivo that compensation requires electroneutral active transport of Na+, K+, and Cl− through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes alter their electrical activity at different times of the day. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.

show abstract

Section: Antiphasic Oscillations In Soluble Protein and Intracellularmentioning

confidence: 69%

Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology

Stangherlin

Wong

Barbiero

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Interestingly, there is no strong correlation between membrane conductance and cytoplasm conductivity. Recent work studying the DEP properties of red blood cells indicated a correlation between membrane conductance, cytoplasm conductivity and membrane potential; however, here the membrane and cytoplasm parameters change asynchronously, suggesting that whilst one may affect the membrane potential, the parameter does not unite both. Similarly, unlike that work the movement of the two parameters is counterpunctual.…”

Section: Resultsmentioning

confidence: 55%

Dielectrophoretic analysis of treated cancer cells for rapid assessment of treatment efficacy

2018

Self Cite

View full text Add to dashboard Cite

Whilst personalized medicine (where interventions are precisely tailored to a patient's genotype and phenotype, as well as the nature and state of the disease) is regarded as an optimal form of treatment, the time and cost associated with it means it remains inaccessible to the greater public. A simpler alternative, stratified medicine, identifies groups of patients who are likely to respond to a given treatment. This allows appropriate treatments to be selected at the start of therapy, avoiding the common "trial and error" approach of replacing a therapy only once it is demonstrated to be ineffective in the patient. For stratification to be effective, tests are required that rapidly predict treatment effectiveness. Most tests use genetic analysis to identify drug targets, but these can be expensive and may not detect changes in the phenotype that affect drug sensitivity. An alternative method is to assess the whole-cell phenotype by evaluating drug response using cells from a biopsy. We assessed dielectrophoresis to assess drug efficacy on short timescales and at low cost. To explore the principle of assessing drug efficacy we examined two cell lines (one expressing EGFR, one not) with the drug Iressa. We then further explored the sensitive cells using combinations of chemotherapeutic and radiotherapeutic therapies. Our results compare with known effects of these cell/treatment combination, and offer the additional benefit over methods such as TUNEL of detecting drug effects such as cell cycle arrest, which do not cause cell death.

show abstract

“…Clinical consequences of period shortening in circadian cycles are evidenced in sleeping sickness caused by Trypanosoma brucei [39]. Noticeably, however, cycling also occurs in the absence of a genome, as shown by intracellular K + levels in human red cells [40]. However rhythms are caused, they allow the necessary time keeping in living beings, or as having stated by Joseph Bass (2017): As energy metabolism being in constant flux, there would be time in biochemical processes, as there is in a central train station [41].…”

Section: Rhythm Generationmentioning

confidence: 99%

Melatonin Relations with Energy Metabolism as Possibly Involved in Fatal Mountain Road Traffic Accidents

Behn

Gregorio

2020

IJMS

View full text Add to dashboard Cite

Previous results evidenced acute exposure to high altitude (HA) weakening the relation between daily melatonin cycle and the respiratory quotient. This review deals with the threat extreme environments pose on body time order, particularly concerning energy metabolism. Working at HA, at poles, or in space challenge our ancestral inborn body timing system. This conflict may also mark many aspects of our current lifestyle, involving shift work, rapid time zone crossing, and even prolonged office work in closed buildings. Misalignments between external and internal rhythms, in the short term, traduce into risk of mental and physical performance shortfalls, mood changes, quarrels, drug and alcohol abuse, failure to accomplish with the mission and, finally, high rates of fatal accidents. Relations of melatonin with energy metabolism being altered under a condition of hypoxia focused our attention on interactions of the indoleamine with redox state, as well as, with autonomic regulations. Individual tolerance/susceptibility to such interactions may hint at adequately dealing with body timing disorders under extreme conditions.

show abstract

Rhythmic potassium transport regulates the circadian clock in human red blood cells

Cited by 71 publications

References 45 publications

Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology

Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology

Dielectrophoretic analysis of treated cancer cells for rapid assessment of treatment efficacy

Melatonin Relations with Energy Metabolism as Possibly Involved in Fatal Mountain Road Traffic Accidents

Contact Info

Product

Resources

About