Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen

Zhang, Zhichao; Lai, Shuang; Wang, Yagang; Li, Liang; Yin, Huadong; Wang, Yan; Zhao, Xiaoling; Li, Diyan; Yang, Mingyao; Zhu, Qing

doi:10.1371/journal.pone.0179019

Cited by 11 publications

(10 citation statements)

References 34 publications

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“…Additionally, transcription of known core clock components showed the classic sinusoidal circadian profile (q < 0.05) with a zenith and nadir at the expected time points. This verifies our previous study which indicated the presence of functional clock in the ESG ( Zhang et al., 2017 ). Together, these results demonstrate the robustness of our data.…”

Section: Discussionsupporting

confidence: 92%

“…Precise timing and rhythmicity in hormone release and tissue sensitivity in the HPG axis is regulated by circadian clocks located at hypothalamus, pituitary ( Olcese et al, 2006 ; Resuehr et al, 2007 ), and ovary ( Fahrenkrug et al, 2006 ; Karman and Tischkau, 2006 ; Zhang et al, 2017 ). There are few studies on the role of endogenous clock in eggshell formation in the ESG.…”

Section: Introductionmentioning

confidence: 99%

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A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken

Zhang

Lai

et al. 2022

Poultry Science

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Circadian timing system controlled the rhythmic events, for example, ovulation and oviposition in chickens. However, how biological clock mediates eggshell formation remains obscure. Here, A 24-h mRNA transcriptome analysis was carried out in the uterus of 18 chickens with similar oviposition time points to identify the rhythmic genes and to reveal critical genes and biological pathways involved in the eggshell biomineralization. JTK_CYCLE analysis and real-time PCR revealed a total of 1,793 genes from the sequencing database with 23,513 genes (FPKM>1) were rhythmic genes regulating the rhythmic system and the expression of typical clock genes Per2, Cry1, Bmal1, Clock, Per3 , and Rev-erbβ were rhythmically expressed, which suggested that endogenous clock in uterus might control the eggshell mineralization. Time of peak expression of the rhythmic genes was analyzed based on their acrophase. The main phases clustered at the periods from Zeitgeber time 0 ( ZT0 ) to ZT4 (6:00–10:00) and from ZT10 to ZT14 (16:00-20:00). The rhythmic genes were annotated to the following Gene Ontology terms rhythmic process, lyase, ATP binding, cell membrane component. KEGG pathway enrichment analysis revealed the top 15 rhythmic genes were involved in vital biological pathways, including syndecan (1, 2, 3)-mediated signaling, post-translational regulation of adheres junction stability and disassembly, FoxO family signaling, TGF-β receptor and transport of small molecular pathways. 166 of total 1,235 genes (13.4%) were defined as rhythmic transfer factors ( TFs ) and they were investigated expression time distribution of cis-elements of circadian clock system D-box, E-box, B-site, and Y-Box within 24 h. Results indicated that rhythmic TFs at each phase are potential drivers of their circadian transcription activities. Compared with the control, the expression abundances of ion transport elements SCNN1G, CA2, SPP1 , and ATP1B1 were significantly decreased after the interference of Bmal1 gene in synchronized uterine tubular gland cells. Clock genes changed their expression along with the eggshell formation, indicating that there is circadian clock in the uterus of chicken and it regulates the expression of eggshell formation genes.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken

Zhang

Lai

et al. 2022

Poultry Science

Self Cite

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“…The biological clock is a cell-autonomous system that coordinates physiology and metabolism to align behavioral processes with the day/night cycle (Bass and Takahashi, 2010 ). The rhythm is important for reproduction through its effect on ovulation (Zhang et al, 2017 ). We observed that the egg-laying peak occurred at 4 p.m. for the female B. dorsalis , consistent with a previous report (Yang et al, 1994 ).…”

Section: Discussionmentioning

confidence: 99%

Olfactory Plasticity: Variation in the Expression of Chemosensory Receptors in Bactrocera dorsalis in Different Physiological States

Jin

Zhou

et al. 2017

Front. Physiol.

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Changes in physiological conditions could influence the perception of external odors, which is important for the reproduction and survival of insect. With the alteration of physiological conditions, such as, age, feeding state, circadian rhythm, and mating status, insect can modulate their olfactory systems accordingly. Ionotropic, gustatory, and odorant receptors (IR, GR, and ORs) are important elements of the insect chemosensory system, which enable insects to detect various external stimuli. In this study, we investigated the changes in these receptors at the mRNA level in Bactrocera dorsalis in different physiological states. We performed transcriptome analysis to identify chemosensory receptors: 21 IRs, 12 GRs, and 43 ORs were identified from B. dorsalis antennae, including almost all previously known chemoreceptors in B. dorsalis and a few more. Quantitative real-time polymerase chain reaction analysis revealed the effects of feeding state, mating status and time of day on the expression of IR, GR, and OR genes. The results showed that expression of chemosensory receptors changed in response to different physiological states, and these changes were completely different for different types of receptors and between male and female flies. Our study suggests that the expressions of chemosensory receptors change to adapt to different physiological states, which may indicate the significant role of these receptors in such physiological processes.

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“…Thus, the circadian clock is a major regulator of a wide range of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine functions, and coordinates rhythmic gene expression in multiple tissues. In chickens, circadian/clock genes include PER, CRY, BMAL genes, which are expressed in several tissues including the thyroid gland, multiple oscillatory systems (the retina, the pineal and the hypothalamus) [17] but also reproductive tissues [18, 19]. The expression/activity of these genes/proteins is crucial to synchronize gene transcription/translation of key metabolic pathways thereby orchestrating the time course of physiological and behavioral processes.…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of transcriptomic data related to the liver of laying hens: from physiological basics to newly identified functions

et al. 2019

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BackgroundAt sexual maturity, the liver of laying hens undergoes many metabolic changes to support vitellogenesis. In published transcriptomic approaches, hundreds of genes were reported to be overexpressed in laying hens and functional gene annotation using gene ontology tools have essentially revealed an enrichment in lipid and protein metabolisms. We reanalyzed some data from a previously published article comparing 38-week old versus 10-week old hens to give a more integrative view of the functions stimulated in the liver at sexual maturity and to move beyond current physiological knowledge. Functions were defined based on information available in Uniprot database and published literature.ResultsOf the 516 genes previously shown to be overexpressed in the liver of laying hens, 475 were intracellular (1.23–50.72 fold changes), while only 36 were predicted to be secreted (1.35–66.93 fold changes) and 5 had no related information on their cellular location. Besides lipogenesis and protein metabolism, we demonstrated that the liver of laying hens overexpresses several clock genes (which supports the circadian control of liver metabolic functions) and was likely to be involved in a liver/brain/liver circuit (neurotransmitter transport), in thyroid and steroid hormones metabolisms. Many genes were associated with anatomical structure development, organ homeostasis but also regulation of blood pressure. As expected, several secreted proteins are incorporated in yolky follicles but we also evidenced that some proteins are likely participating in fertilization (ZP1, MFGE8, LINC00954, OVOCH1) and in thyroid hormone maturation (CPQ). We also proposed that secreted proteins (PHOSPHO1, FGF23, BMP7 but also vitamin-binding proteins) may contribute to the development of peripheral organs including the formation of medullar bones to provide labile calcium for eggshell formation. Thirteen genes are uniquely found in chicken/bird but not in human species, which strengthens that some of these genes may be specifically related to avian reproduction.ConclusionsThis study gives additional hypotheses on some molecular actors and mechanisms that are involved in basic physiological function of the liver at sexual maturity of hen. It also revealed some additional functions that accompany reproductive capacities of laying hens, and that are usually underestimated when using classical gene ontology approaches.

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Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen

Cited by 11 publications

References 34 publications

A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken

A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken

Olfactory Plasticity: Variation in the Expression of Chemosensory Receptors in Bactrocera dorsalis in Different Physiological States

Integrative analysis of transcriptomic data related to the liver of laying hens: from physiological basics to newly identified functions

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