Rhodopeptins, Novel Cyclic Tetrapeptides with Antifungal Activities from Rhodococcus sp. III. Synthetic Study of Rhodopeptins.

Chiba, Hiroyuki; Agematu, Hitosi; Sakai, Kazuya; Dobashi, Kazuyuki; Yoshioka, Takeo

doi:10.7164/antibiotics.52.710

Cited by 20 publications

(22 citation statements)

References 19 publications

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“…RJB may be involved in control of the expression of one or more of these clusters. Although a few Rhodococcus antimicrobials are known 41 – 44 this genus has remained largely unexplored for production of secondary metabolites. In this work, we detected bioactivity of R .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of a Rhodococcus jostii RHA1 γ-butyrolactone(-like) signalling molecule and its main biosynthesis gene gblA

Ceniceros

Dijkhuizen

Petrusma

2017

Sci Rep

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Rhodococcus genome sequence analysis has revealed a surprisingly large (and unexplored) potential for the production of secondary metabolites. Also, putative γ-butyrolactone gene clusters have been identified in some Rhodococci. These signalling molecules are known to regulate secondary metabolism in Streptomyces. This work provides evidence for synthesis of a γ-butyrolactone(-like) molecule by Rhodococci (RJB), the first report in the Rhodococcus genus. The Rhodococcus jostii RHA1 RJB molecule was detected by a reporter system based on the γ-butyrolactone receptor protein (ScbR) of Streptomyces coelicolor. This RJB is structurally identical to 6-dehydro SCB2, the predicted precursor of the S. coelicolor γ-butyrolactone SCB2. The R. jostii RHA1 key RJB biosynthesis gene was identified (gblA): Deletion of gblA resulted in complete loss of RJB synthesis whereas higher RJB levels were detected when gblA was overexpressed. Interaction of the RJB molecule with ScbR indicates that communication may occur between these two Actinomycete genera in their natural habitat. Furthermore, RJB may provide a highly relevant tool for awakening cryptic secondary metabolic gene clusters in Rhodococci. This study provides preliminary evidence that R. jostii RHA1 indeed synthesizes diffusible molecules with antimicrobial activity, but a possible role for RJB in this remains to be established.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of a Rhodococcus jostii RHA1 γ-butyrolactone(-like) signalling molecule and its main biosynthesis gene gblA

Ceniceros

Dijkhuizen

Petrusma

2017

Sci Rep

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“…Additionally, multiple Rhodococcus strains have also been reported to produce antibiotics [ 21 ]. Four (groups of) rhododoccal natural products with antimicrobial activity have been described in literature; lariantin peptide antibiotics with anti-mycobacterial activity [ 22 ], the polyketide aurachin RE from Rhodococcus erythropolis JCM 6824 (which has a structure similar to that of aurachin C from the Gram-negative organism Stigmatella aurantiaca [ 23 , 24 ]), a group of peptide antifungals named rhodopeptins [ 25 ] and the recently described humimycins [ 26 ]. The gene cluster responsible for the synthesis of rhodopeptins has not yet been identified.…”

Section: Introductionmentioning

confidence: 99%

Genome-based exploration of the specialized metabolic capacities of the genus Rhodococcus

et al. 2017

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BackgroundBacteria of the genus Rhodococcus are well known for their ability to degrade a large range of organic compounds. Some rhodococci are free-living, saprophytic bacteria; others are animal and plant pathogens. Recently, several studies have shown that their genomes encode putative pathways for the synthesis of a large number of specialized metabolites that are likely to be involved in microbe-microbe and host-microbe interactions. To systematically explore the specialized metabolic potential of this genus, we here performed a comprehensive analysis of the biosynthetic coding capacity across publicly available rhododoccal genomes, and compared these with those of several Mycobacterium strains as well as that of their mutual close relative Amycolicicoccus subflavus.ResultsComparative genomic analysis shows that most predicted biosynthetic gene cluster families in these strains are clade-specific and lack any homology with gene clusters encoding the production of known natural products. Interestingly, many of these clusters appear to encode the biosynthesis of lipopeptides, which may play key roles in the diverse environments were rhodococci thrive, by acting as biosurfactants, pathogenicity factors or antimicrobials. We also identified several gene cluster families that are universally shared among all three genera, which therefore may have a more ‘primary’ role in their physiology. Inactivation of these clusters by mutagenesis might help to generate weaker strains that can be used as live vaccines.ConclusionsThe genus Rhodococcus thus provides an interesting target for natural product discovery, in view of its large and mostly uncharacterized biosynthetic repertoire, its relatively fast growth and the availability of effective genetic tools for its genomic modification.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3966-1) contains supplementary material, which is available to authorized users.

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“…Members of the genus Rhodococcus have been reported to show antifungal activity in vitro against plant-pathogenic fungi ( Chiba et al, 1999 ; Iwatsuki et al, 2007 ; Santos et al, 2020 ) and RL1 reduced growth of F. oxysporum in vitro , but showed no inhibition against R. solani and F. culmorum ( Supplementary Figure 4 ). Further studies using model plants will reveal the full potential of RL1 as biocontrol agent against plant pathogenic fungi.…”

Section: Discussionmentioning

confidence: 99%

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

et al. 2021

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Stress tolerant, plant-associated bacteria can play an important role in maintaining a functional plant microbiome and protecting plants against various (a)biotic stresses. Members of the stress tolerant genus Rhodococcus are frequently found in the plant microbiome. Rhodococcus qingshengii RL1 was isolated from Eruca sativa and the complete genome was sequenced, annotated and analyzed using different bioinformatic tools. A special focus was laid on functional analyses of stress tolerance and interactions with plants. The genome annotation of RL1 indicated that it contains a repertoire of genes which could enable it to survive under different abiotic stress conditions for e.g., elevated mercury concentrations, to interact with plants via root colonization, to produce phytohormones and siderophores, to fix nitrogen and to interact with bacterial signaling via a LuxR-solo and quorum quenching. Based on the identified genes, functional analyses were performed in vitro with RL1 under different growth conditions. The R. qingshengii type strain djl6 and a closely related Rhodococcus erythropolis BG43 were included in the experiments to find common and distinct traits between the strains. Genome based phylogenetic analysis of 15 available and complete R. erythropolis and R. qingshengii genome sequences revealed a separation of the R. erythropolis clade in two subgroups. First one harbors only R. erythropolis strains including the R. erythropolis type strain. The second group consisted of the R. qingshengii type strain and a mix of R. qingshengii and R. erythropolis strains indicating that some strains of the second group should be considered for taxonomic re-assignment. However, BG43 was clearly identified as R. erythropolis and RL1 clearly as R. qingshengii and the strains had most tested traits in common, indicating a close functional overlap of traits between the two species.

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Rhodopeptins, Novel Cyclic Tetrapeptides with Antifungal Activities from Rhodococcus sp. III. Synthetic Study of Rhodopeptins.

Cited by 20 publications

References 19 publications

Molecular characterization of a Rhodococcus jostii RHA1 γ-butyrolactone(-like) signalling molecule and its main biosynthesis gene gblA

Molecular characterization of a Rhodococcus jostii RHA1 γ-butyrolactone(-like) signalling molecule and its main biosynthesis gene gblA

Genome-based exploration of the specialized metabolic capacities of the genus Rhodococcus

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

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