Rhodiola rosea ability to enrich cellular antioxidant defences of cultured human keratinocytes

Abstract

Keratinocytes are cells strongly exposed to oxidative stress, but normally good equipped for antioxidant responses. However, it has long been suggested that exogenous antioxidants could play a useful role in minimizing the adverse skin responses associated with such oxidant species. In this work it was paid attention to the extract of Rhodiola rosea L. roots by using the phytocomplex as a whole because of the important activity of its composition and mutual distribution of its components. We have measured the protection afforded by the extract to reduced glutathione levels, glyceraldehyde-3-phosphate dehydrogenase activity, and thiobarbituric acid reactive substances levels in cultured human keratinocytes (NCTC 2544) exposed to different oxidative insults: Fe(II)/ascorbate, Fe(II)/H(2)O(2), and tert-butyl-hydroperoxide. We also have investigated the influence of the R. rosea extract on the production of intracellular reactive oxygen species and on the activity of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase). Furthermore, we have demonstrated that R. rosea extract was able to increase in a time- and dose-dependent manner the activity of the trans plasma membrane oxido reductase activity as an indirect evaluation of the intracellular redox status and this effect was already evident with small concentration of the extract and in a long time. As a result, NCTC 2544 are able to better counteract to several oxidative insults if incubated with R. rosea extract demonstrating a very good antioxidant activity of this phytocomplex.

Alleviation of Kidney Damage Induced by Unilateral Ureter Obstruction in Rats byRhodiola rosea

“…6 R. rosea exerts antioxidant effects and thereby reduced tissue injury, resulting from oxidative stress. 7,8 However, the efficacy of R. rosea in renal tissue damage after ureter obstruction has not been investigated. In the present study, we assessed the efficacy of R. rosea at alleviating the renal damage induced by unilateral ureter obstruction (UUO) in rats.…”

“…A study carried out on keratinocytes revealed that R. rosea increased antioxidant enzyme levels, demonstrating reliable antioxidant activity. 7 Palumbo et al reported that R. rosea had antioxidant properties and acted as a neuroprotector of cortical neurons. 8 Our results also indicate that R. rosea possesses antioxidant activity.…”

Salidroside Decreases Atherosclerotic Plaque Formation in Low-Density Lipoprotein Receptor-Deficient Mice

“…Rhodiola rosea has long been used as a medicinal plant and has been reported to have various pharmacological properties, including antifatigue and antistress activity [3], anticancer, antioxidant and immune enhancing and stimulating sexual activity [4], anti-inflammation [5], improvement of glucose and lipid metabolism [6, 7], antiarrhythmic effect [8], and enhancement of angiogenesis [9]. However, the effects of Rhodiola rosea on atherosclerotic lesions formation are still unclear.…”

Inhibitory Mechanism of Salidroside on Tyrosinase

“…Salidroside, a compound with the chemical structure of phenol glycosides, is an important kind of flavonoids extracted from the root of Rhodiola rosea [21], existed in seed coat of walnut and so on [22,23]. Besides roles in anti-inflammation [24], anti-oxidation [25], scavenging free radical [20], salidroside also can reduce ischemiamediated myocardial damage [26], moreover, it has been shown that salidroside has ability to inhibit the activity of tyrosinase which is the main rate-limiting enzyme in the process of melanin synthesis [20].…”