Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy

Nanoemulsion-templated polylelectrolyte multifunctional nanocapsules for DNA entrapment and bioimaging

“…The main techniques of genetic delivery involve the use of genetically modified viruses, non-viral gene transfers and formation of DNA complexes with inorganic salts, polycations, surfactants, or lipids [1,[4][5][6]. Up to now, viral vectors have been the most frequently applied, but they have several potential disadvantages, such as immunogenicity, insertional mutations, and potential pathogenicity [2].…”

“…The deposition of DNA and encapsulation of NIR fluorescent cyanine in newly designed oil-core nanocarriers with naturally occurring CHIT shell can make those cargoes more biocompatible thus help in making them attractive as alternatives for more traditional fluorescent materials used for in vitro cellular applications of genetic material [6]. These promising results indicate the protective effect of the applied nanocapsules and the potential application of the studied nanoemulsion-templated systems for DNA delivery and bioimaging as well as constitute a base for further in vivo studies that should be performed.…”

Mitochondrial Genetic Disorders: Challenges & Opportunities

“…This includes biodegradable polymeric nanoparticle platforms, such as PLGA-PEG nanoparticles, surface modified with TPP moiety [29,30]; mitochondrial targeting gold peptides [31]; TPP conjugated poly (amidoamine) dendrimers [32]; multifunctional enveloped mesoporous silica nanoparticles (MSNs) modified with TPP, peptides and coated with pH-sensitive PEG-PLL (DMA) polymer [33]; rhodamine-based plasmid DNA nanoparticles [34] and functional DOX-nanoparticles prepared with folate-terminated polyrotaxanes (FPRs) and dequalinium (DQA) [35]. These nanocarrier formulations represent some novel techniques delivering various small molecules, peptides & DNA to mitochondria and along with previously mentioned delivery systems offer promise and potential to routinely target mitochondria as a sub-cellular target.…”

FluoroNanogold: an important probe for correlative microscopy

“…However, problems with quantum dots, including stability, blinking, and low electron density, have limited their use as correlative probes. The use of quantum dots for labeling in biological systems has been reviewed and will not be discussed further ( [16,25,26,41,50,73]).…”