129the biochemical level, the metabolic flux assay provides OCR and ECAR data and information on other mitochondrial 130 bioenergetic properties (by Mito Stress Test). The cells are then stained with nuclear and mitochondrial dyes that 131 provide information on the cellular properties noted.
132
METHODS 133Cell culture. PA-TU-8902, MIA PaCa2, S2-013, and T3M4 pancreatic cancer cell lines were 134 cultured in DMEM supplemented with 10% FBS. S2-013 ρ 0 cells were supplied additionally with 135 10 µg/ml EtBr, 100 µg/ml pyruvate and 50 µg/ml uridine. The VARI068 breast cancer cell line 136 was maintained in RPMI1640 supplied with 10% FBS. Cell lines were STR profiled and routinely 137 tested for mycoplasma. 138
Chemicals and probes.