Rho1 regulates<i>Drosophila</i>adherens junctions independently of p120ctn

Fox, Donald T.; Homem, Catarina C.F.; Myster, Steven H.; Wang, Fei; Bain, E. Eugene; Peifer, Mark

doi:10.1242/dev.02056

Cited by 49 publications

(47 citation statements)

References 55 publications

(72 reference statements)

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“…These cells assemble an actin-myosin cable at their dorsal-most edge (Edwards et al, 1997;Jacinto et al, 2002a;Kaltschmidt et al, 2002;Kiehart et al, 2000) that seeds cellular protrusions, filopodia and lamellipodia, which play adhesive and segment-matching roles in the late stages of DC (Jacinto et al, 2000;Liu et al, 2008;Millard and Martin, 2008). A number of studies have implicated interactions between the cytoskeleton and cell-adhesion systems in the development and coordination of these movements (Bloor and Kiehart, 2002;Fox et al, 2005;Franke et al, 2005;Gorfinkiel and Martínez Arias, 2007;Grevengoed et al, 2001;Magie et al, 2002;Murray et al, 2006;Takahashi et al, 2005). Furthermore, laser ablation of small groups of cells in the AS or the epidermis have revealed some of the forces involved in DC.…”

Section: Introductionmentioning

confidence: 99%

Mechanical control of global cell behaviour during dorsal closure inDrosophila

et al. 2009

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Halfway through embryonic development, the epidermis of Drosophila exhibits a gap at the dorsal side covered by an extraembryonic epithelium, the amnioserosa (AS). Dorsal closure (DC) is the process whereby interactions between the two epithelia establish epidermal continuity. Although genetic and biomechanical analysis have identified the AS as a force-generating tissue, we do not know how individual cell behaviours are transformed into tissue movements. To approach this question we have applied a novel image-analysis method to measure strain rates in local domains of cells and performed a kinematic analysis of DC. Our study reveals spatial and temporal differences in the rate of apical constriction of AS cells. We find a slow phase of DC, during which apical contraction of cells at the posterior end predominates, and a subsequent fast phase, during which all the cells engage in the contraction, which correlates with the zippering process. There is a radial gradient of AS apical contraction, with marginal cells contracting earlier than more centrally located cells. We have applied this analysis to the study of mutant situations and associated a particular genotype with quantitative and reproducible changes in the rate of cell contraction and hence in the overall rate of the process. Our mutant analysis reveals the contribution of mechanical elements to the rate and pattern of DC.

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Section: Introductionmentioning

confidence: 99%

Mechanical control of global cell behaviour during dorsal closure inDrosophila

et al. 2009

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“…Several studies have hinted at the importance of AJs and their regulation during DC (Bloor and Kiehart, 2002;Fox et al, 2005;Grevengoed et al, 2001;Magie et al, 2002;McEwen et al, 2000;Murray et al, 2006;Takahashi et al, 2005). For example, defects associated with genetic interactions between shotgun (shg), the gene encoding E-cadherin in Drosophila, and actin regulators such as the small GTPase Rho (Fox et al, 2005) and the non-receptor tyrosine kinases Src (Takahashi et al, 2005) or Abl (Grevengoed et al, 2001), suggest that some aspect of the AJs is crucial for DC.…”

Section: Introductionmentioning

confidence: 99%

“…For example, defects associated with genetic interactions between shotgun (shg), the gene encoding E-cadherin in Drosophila, and actin regulators such as the small GTPase Rho (Fox et al, 2005) and the non-receptor tyrosine kinases Src (Takahashi et al, 2005) or Abl (Grevengoed et al, 2001), suggest that some aspect of the AJs is crucial for DC. However, although some shg mutations that reach the DC stages (Tepass et al, 1996;Uemura et al, 1996), there has not been a full study of the role of AJs in this epithelial sheet movement.…”

Section: Introductionmentioning

confidence: 99%

Requirements for adherens junction components in the interaction between epithelial tissues during dorsal closure inDrosophila

Gorfinkiel

Arias

2007

Journal of Cell Science

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Dynamic interactions between epithelial sheets are a regular feature of morphogenetic processes. Dorsal closure in Drosophila relies on the coordinated movements of two epithelia, the epidermis and the amnioserosa, and provides an excellent model system for a genetic and cell biological approach. Here, we have analyzed the contribution of junctional organization of these epithelia to dorsal closure. We observe a stringent requirement for adherens junctions at the leading edge, the interface between the amnioserosa and the epidermis, for the transmission of the forces generated during the process. We also find that interactions between Armadillo and E-cadherin play an important role in maintaining the adhesion at the leading edge, revealing the particular dynamics of this interface. Our results show that regulated cell adhesion is a crucial element of the interactions that shape epithelial sheets in morphogenetic processes.

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“…The stablisation of cells within epithelia could explain other p120-catenin mutant phenotypes, such as defects in retinal patterning and rates of cell movement during dorsal closure (Fox et al, 2005;Larson et al, 2008). Combined loss of p120-catenin-binding sites and endocytic motifs from vertebrate cadherins reduced cell migration upon VEGF induction (Nanes et al, 2012) and disrupted morphogenetic movements in Xenopus embryos (Ciesiolka et al, 2004), suggesting that the function of p120-catenin in cell rearrangements is conserved between Drosophila and vertebrates.…”

Section: Two Pathways For E-cad Endocytosismentioning

confidence: 99%

“…Nonetheless, loss of p120-catenin results in developmental defects in Drosophila, including slowed morphogenetic movements during dorsal closure (Fox et al, 2005) and retinal patterning defects (Larson et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Drosophila p120-catenin is crucial for endocytosis of the dynamic E-cadherin–Bazooka complex

Bulgakova¹,

Brown²

2016

Development

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The intracellular functions of classical cadherins are mediated through the direct binding of two catenins: β-catenin and p120-catenin (also known as CTNND1 in vertebrates, and p120ctn in Drosophila). Whereas β-catenin is crucial for cadherin function, the role of p120-catenin is less clear and appears to vary between organisms. We show here that p120-catenin has a conserved role in regulating the endocytosis of cadherins, but that its ancestral role might have been to promote endocytosis, followed by the acquisition of a new inhibitory role in vertebrates. In Drosophila, p120-catenin facilitates endocytosis of the dynamic E-cadherin-Bazooka subcomplex, which is followed by its recycling. The absence of p120-catenin stabilises this subcomplex at the membrane, reducing the ability of cells to exchange neighbours in embryos and expanding cell-cell contacts in imaginal discs.

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Rho1 regulatesDrosophilaadherens junctions independently of p120ctn

Cited by 49 publications

References 55 publications

Mechanical control of global cell behaviour during dorsal closure inDrosophila

Mechanical control of global cell behaviour during dorsal closure inDrosophila

Requirements for adherens junction components in the interaction between epithelial tissues during dorsal closure inDrosophila

Drosophila p120-catenin is crucial for endocytosis of the dynamic E-cadherin–Bazooka complex

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