Rho Signaling-Directed YAP/TAZ Regulation Encourages 3D Spheroid Colony Formation and Boosts Plasticity of Parthenogenetic Stem Cells

A 3D approach to reproduction

“…A study recently performed in our laboratory further supports these observations, and demonstrates that YAP/TAZ activity is significantly up-regulated in parthenogenetic ESCs and compartmentalized to the nucleus [42]. This appears to be related to the exclusive maternal origin of these cells and their inability to give rise to functional TE.…”

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

“…Altogether, the representative results here reported demonstrate that this two-step strategy is highly efficient and robust, and the use of a PTFE micro-bioreactor not only boosts high plasticity, but also allows its stable, long-term maintenance in the mammalian species considered. We recently demonstrated that these beneficial effects are related to the activation of the Hippo-signaling pathway and its mechanotransduction-related cues 10 , that have been previously shown to have a key role in the active regulation of cell pluripotency [28][29][30] . The two most critical steps for a successful procedure are the rigorous maintenance of cells at 37 °C, at all times, including their handling under the sterile laminar flow and the microscope and the use of a correct cell number/liquid volume rate during micro-bioreactor production, that may vary according to the specific cell type used.…”

A postzygoticGNA13variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome

“…Here, we show that the R200K Gα13 mutant induces YAP, but not TAZ, nuclear translocation causing its activation and upregulation of the ARPC5 gene involved in actin polymerization and migration. YAP/TAZ nuclear accumulation has previously been linked to the activation of the RHOA pathway 19 . Thus, the R200K Gα13 variant activates the YAP signalling pathway most likely through RHOA hyperactivation.…”