RhMYB108, an R2R3-MYB transcription factor, is involved in ethylene- and JA-induced petal senescence in rose plants

Abstract

Rose (Rosa hybrida) plants are major ornamental species worldwide, and their commercial value greatly depends on their open flowers, as both the quality of fully open petals and long vase life are important. Petal senescence can be started and accelerated by various hormone signals, and ethylene is considered an accelerator of petal senescence in rose. To date, however, the underlying mechanism of signaling crosstalk between ethylene and other hormones such as JA in petal senescence remains largely unknown. Here, we isolated RhMYB108, an R2R3-MYB transcription factor, which is highly expressed in senescing petals as well as in petals treated with exogenous ethylene and JA. Applications of exogenous ethylene and JA markedly accelerated petal senescence, while the process was delayed in response to applications of 1-MCP, an ethylene action inhibitor. In addition, silencing of RhMYB108 alter the expression of SAGs such as RhNAC029, RhNAC053, RhNAC092, RhSAG12, and RhSAG113, and finally block ethylene-and JA-induced petal senescence. Furthermore, RhMYB108 was identified to target the promoters of RhNAC053, RhNAC092, and RhSAG113. Our results reveal a model in which RhMYB108 functions as a receptor of ethylene and JA signals to modulate the onset of petal senescence by targeting and enhancing senescence-associated gene expression.

An R2R3-MYB Transcription Factor RmMYB108 Responds to Chilling Stress of Rosa multiflora and Conferred Cold Tolerance of Arabidopsis

“…In Prunus mum e, PmMYB108 (90% sequence similarity with RmMYB108 ) is positively associated with organ color ( Zhang et al, 2018 ). In R. chinensis , RcMYB108 , which is homologous to RmMYB108 (94.17% sequence similarity), was upregulated during petal senescence and shedding, and silencing of RcMYB108 altered the expression of senescence-associated genes and blocked ethylene- and JA-induced petal senescence ( Zhang et al, 2019 ). Interestingly, when Arabidopsis seeds were cultured at 4°C for 6 months, we found that the speed of seed germination and plant growth was greatly reduced (data not shown).…”

Mediation of JA signalling in glandular trichomes by the woolly/SlMYC1 regulatory module improves pest resistance in tomato

“…The recombinant constructs were co‐transformed into yeast strain EGY48 and the transformed yeast cells were incubated on SD/‐Trp‐Ura medium for 3 days at 30 °C. The transformed yeast cells were then transferred to the SD/‐Trp‐Ura + X‐gal medium and incubated for 5 days to test the interaction between the protein and the promoters of TPSs (Zhang et al ., 2019).…”

Molecular Evidences for the Interactions of Auxin, Gibberellin, and Cytokinin in Bent Peduncle Phenomenon in Rose (Rosa sp.)

“…Briefly, total RNA was isolated from samples with three biological repeats, and 2 µL of the first-strand cDNA was used as template with the Step One Plus TM real-time PCR system (Applied Biosystems) using KAPA™ SYBRR FAST quantitative PCR kits (Kapa Biosystems). The RhUbi2 housekeeping gene was used as standard, which was validated in previous studies [47,48]. The primers used for determining transcript abundance are listed in Supplementary Table S3.…”