Rhizospheric PGPR Strains of Wheat, Barley and Trefoil Grown in Ağrı Province

Abstract

The present study aimed to isolate potential plant growth rhizobacteria (PGPR) from various crops in agricultural areas in Ağrı-Turkey and their characterization. For this goal, rhizospheric soil samples of wheat, barley and trefoil were collected from 12 different locations in Ağrı province. Bacteria isolation studies were carried out with these rhizospheric samples. Then, to determine the PGPR properties of each isolate; nitrogen fixation, phosphate dissolution, siderophore, ammonia and HCN production tests were performed. Molecular identification of active isolates determined as suitable for development of biofertilizers, biostimulants and/or bioprotectants was done by PCR and sequencing applications performed with universal 16S rRNA primers. According to the results, 29 potential PGPR isolates were determined and their molecular characterization was done. These isolates were distributed in Arthrobacter, Bacillus, Brevibacterium, Enterobacter, Pantoea, Pseudomonas, Microbacterium and Stenotrophomonas according to their 16S rRNA sequence similarities.

Community-forming traits play role in effective colonization of plant-growth-promoting bacteria and improved plant growth

“…The results indicated that Bacillus was the predominant genus, followed by Enterobactor, and others including Pantoea, Microbacterium, Stenotrophomonas, and Pseudomonas. This result is consistent with the findings of Suman et al (2020); Alaylar et al (2021) and Sharma et al (2023) where most of the rhizospheric and endophytic culturable microbes were dominated by the Bacilli group.…”