Rhizosphere bacteria community and functions under typical natural halophyte communities in North China salinized areas

Abstract

Soil salinity is a serious environmental issue in arid China. Halophytes show extreme salt tolerance and are grow in saline-alkaline environments. There rhizosphere have complex bacterial communities, which mediate a variety of interactions between plants and soil. High-throughput sequencing was used to investigated rhizosphere bacterial community changes under the typical halophyte species in arid China. Three typical halophytes were Leymus chinensis (LC), Puccinellia tenuiflora (PT), Suaeda glauca (SG). The dominant phyla were Proteobacteria, Actinobacteria, Chloroflexi, Gemmatimonadetes, Acidobacteria and Bacteroidetes, Suaeda glauca rhizosphere has stronger enrichment of Nitrospirae and Cyanobacteria. The Ace, Chao and Shannon indices were significantly higher in soils under LC and SG (P<0.05). Functional predictions, based on 16S rRNA gene by PICRUSt, indicated that Energy metabolism, Amino acid metabolism, Carbohydrate metabolism and Fatty acid metabolism are dominant bacterial functions in three halophytes rhizosphere soil. Carbon metabolism, Oxidative phosphorylation, Methane metabolism, Sulfur metabolism and Nitrogen metabolism in SG were significantly higher than that in LC and PT. Regression analysis revealed that rhizosphere soil bacterial community structure is influenced by soil organic matter (SOM) and soil water content (SWC), while soil bacterial community diversity is affected by soil pH. This study contributes to our understanding of the distribution characteristics and metabolic functions under different halophyte rhizosphere bacterial communities, and will provide references for the use of rhizosphere bacteria to regulate the growth of halophytes and ecological restoration of saline soil.

The effect of white grub (Maladera Verticalis) larvae feeding on rhizosphere microbial characterization of aerobic rice (Oryza sativa L.) in Puer City, Yunnan Province, China

“…M. verticalis larvae feeding significantly depleted the enrichment of the pathway of metabolism of carbohydrate metabolism at KEGG level 2. Carbohydrate metabolism is the dominant bacterial function in halophytes rhizosphere bacteria and light-exposure rhizosphere bacteria [ 63 , 64 ]. Notably, rhizosphere bacteria were significantly affected by soil physicochemical characteristics [ 65 , 66 ].…”

Effects of NaHCO3 Stress on Black Locust (Robinia pseudoacacia L.) Physiology, Biochemistry, and Rhizosphere Bacterial Communities

“…Rhizobacteria play a crucial role in plant growth and metabolism. Under saline-alkali stress, the growth of rhizosphere microorganisms was inhibited and the bacterial community was changed [46,47]. This study utilizes 16S rRNA amplicon sequencing technology to investigate the rhizobacterial community structure of plants under NaHCO 3 stress.…”