Rhizosphere and bulk soil enzyme activities in a Nothotsuga longibracteata forest in the Tianbaoyan National Nature Reserve, Fujian Province, China

Shihong, Xiao; Huiming, You; Wang, You; Liu, Jinshan; Cai, Chang; Wu, Jiangjiexing; Ji, Zhirong; Shu, Zhan; Hu, Zhesen; Zhang, Zhongrui; He, Dong

doi:10.1007/s11676-016-0334-y

Cited by 24 publications

(11 citation statements)

References 35 publications

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“…On the other hand, the activities of β-glucosidase, protease and alkaline phosphatase in rhizosphere soil were significantly higher than those in bulk soil (Figure 1). The rhizosphere effect was likely due to physiological activities of plant roots under HM stress condition, which could cause the roots to release large amounts of enzymes into the rhizosphere soil [54]. Meanwhile, microorganisms adhering plant roots were able to secrete various enzymes to the surrounding soil unceasingly.…”

Section: Discussionmentioning

confidence: 99%

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Yang

Dong

Cao

et al. 2017

Forests

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Abstract:The toxic effects of heavy metal (HM) contamination on plant metabolism and soil microorganisms have been emphasized recently; however, little is known about the differences in soil physical, chemical, and biological properties between bulk and rhizosphere soils contaminated with HMs in forest ecosystem. The present study was conducted to evaluate the rhizosphere effect on soil properties, enzyme activities and bacterial communities associated with Robinia pseudoacacia L. along a HM contamination gradient. Soil organic matter (SOM), available nitrogen (AN) and phosphorus (AP) contents were significantly higher in rhizosphere soil than those in bulk soil at HM contaminated sites (p < 0.05). Compared to bulk soil, activities of four soil enzymes indicative of C cycle (β-glucosidase), N cycle (protease, urease) and P cycle (alkaline phosphatase) in rhizosphere soil across all study sites increased by 47.5%, 64.1%, 52.9% and 103.8%, respectively. Quantitative PCR (qPCR) and restriction fragment length polymorphism (RFLP) were used to determine the relative abundance, composition and diversity of bacteria in both bulk and rhizosphere soils, respectively. The copy number of bacterial 16S rRNA gene in bulk soil was significantly lower than that in rhizosphere soil (p < 0.05), and it had significantly negative correlations with total/DTPA-extractable Pb concentrations (p < 0.01). Alphaproteobacteria, Gammaproteobacteria and Firmicutes were the most dominant groups of bacteria at different study sites. The bacterial diversity index of Species richness (S) and Margalef (d Ma ) were significantly higher in rhizosphere soil compared with those in bulk soil, although no difference could be found in Simpson index (D) between bulk and rhizosphere soils (p > 0.05). Redundancy analysis (RDA) results showed that soil pH, EC, SOM and total/DTPA-extractable Pb concentrations were the most important variables affecting relative abundance, composition and diversity of bacteria (p < 0.05). Our study highlights the importance of rhizosphere effect on soil nutrient content, enzyme activity, bacterial abundance and community in HM contaminated forest soils. Further study is still required to understand the specific processes in the rhizosphere to achieve a suitable rhizosphere biotechnology for restoration of degraded forest ecosystem.

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Section: Discussionmentioning

confidence: 99%

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Yang

Dong

Cao

et al. 2017

Forests

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“…The potentially active biomass was assessed by SIR based on the activity of soil microbial biomass with supplement of glucose as substrate ( Lipson, Schmidt & Monson, 1999 ). Invertase activity was measured according to Xiao et al (2017) , in which the released glucose through the enzyme lysis was quantified from the absorbance at 508 nm after adding 3,5-dini-trosalicylic acid ( Ladd & Butler, 1972 ). Protease activity was also assayed according to Ladd & Butler (1972) .…”

Section: Methodsmentioning

confidence: 99%

Zanthoxylum bungeanum root-rot associated shifts in microbiomes of root endosphere, rhizosphere, and soil

Liao

Chen

Xiang

et al. 2022

PeerJ

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Root-rot disease has lead to serious reduction in yields and jeopardized the survival of the economically and ecologically important Zanthoxylum bungeanum trees cultured in Sichuan Province. In order to investigate the interaction between the microbiome and the root-rot disease, a metagenomic analysis was performed to characterize the microbial communities and functions in Z. bungeanum root endosphere, rhizosphere and bulk soil with/without root-rot disease. Soil physicochemical properties, microbial population size and enzyme activities were also analyzed for finding their interactions with the root-rot disease. As results, lower total nitrogen (TN) and available phosphorus (AP) contents but higher pH in rhizosphere and bulk soil, as well as lower substrate-induced respiration (SIR) and higher protease activity in bulk soil of diseased trees were found, in comparison with that of healthy trees. Microbial diversity and community composition were changed by root-rot disease in the endosphere, but not in rhizosphere and bulk soils. The endophytic microbiome of diseased trees presented higher Proteobacteria abundance and lower abundances of Bacteroidetes, Firmicutes and dominant fungal phyla. The relative abundances of nitrogen cycle- and carbon cycle-related genes in endophytic microbiomes were different between the diseased and healthy trees. Based on ANOSIM and PCoA, functional profiles (KEGG and CAZy) of microbiomes in rhizosphere and bulk soil shifted significantly between the diseased and healthy trees. In addition, soil pH, TN, AP, SIR, invertase and protease were estimated as the main factors influencing the shifts of taxonomic and functional groups in microbiomes of rhizosphere and bulk soil. Conclusively, the imbalance of root and soil microbial function groups might lead to shifts in the root endosphere-rhizosphere microenvironment, which in turn resulted in Z. bungeanum root-rot.

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“…The higher enzyme activity of the rhizosphere may be due to the physiological activity of the roots, which produce and release large amounts of enzymes under the influence of heavy metals or via the lysis of root cells. Moreover, it may also depend on the physiological metabolic activities of the diverse microbial populations, which produce specific soil enzymes (Gianfreda 2015; Xiao et al 2017;Yang et al 2017).…”

Section: Rhizosphere Effects On Enzyme Activitymentioning

confidence: 99%

“…From the viewpoint of microbial ecology, the rhizosphere is a special unique hot spot in the soil where microorganisms are considerably stimulated by the activity of the roots (Jones et al 2004;Hisinger et al 2006;Egamberdieva et al 2011). The rhizosphere is also an important site of material cycling and energy flow (Xiao et al 2017) and has a significant influence on the availability or solubility of nutrients as well as on the availability of heavy metals (Orroño et al 2012). Because of this, the distribution of heavy metals in rhizosphere soil is more significant for the evaluation of the bioavailability of heavy metals than bulk soil is (Youssef 1997).…”

Section: Introductionmentioning

confidence: 99%

The influence of heavy metals on biological soil quality assessments in the Vaccinium myrtillus L. rhizosphere under different field conditions

2021

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The aim of this study was to determine the influence of heavy metals on biological soil quality assessments in Vaccinium myrtillus L. rhizosphere soil as well as in non-rhizosphere soil from different polluted sites. The presented study was also conducted in order to determine any differences in the soil physicochemical and biological properties between the Vaccinium rhizosphere soil and the non-rhizosphere soil. The content of heavy metals and their potential bioavailability, content of macronutrients, physicochemical soil properties, activity of six soil enzymes and microarthropod communities were determined. Soil organic matter, the levels of C, N and all the studied macronutrients and almost all enzyme activity were significantly higher in the rhizosphere soil than in the non-rhizosphere soil. At the most contaminated site, the content of heavy metals was also higher in the rhizosphere soil, but their bioavailability was lower than in the non-rhizosphere soil. The β-glucosidase and urease activity in the soil correlated most negatively with the examined metals. The levels of two enzymes were also strongly impacted by the organic matter—the C and N levels and pH. The number of microarthropods as well as the QBS (soil biological quality index) and FEMI (abundance-based fauna index) were higher in the rhizosphere soil. The bilberry rhizosphere soil had stronger correlation coefficient values between the measured parameters than the non-rhizosphere soil, which suggests that rhizosphere soil is more sensitive and could be used in the monitoring and assessment of forest ecosystems. β-glucosidase and urease were the most sensitive indicators of the adverse impact of Cd, Zn and Pb. The FEMI index seems to be a better indicator than the QBS for identifying differences in soil quality.

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Rhizosphere and bulk soil enzyme activities in a Nothotsuga longibracteata forest in the Tianbaoyan National Nature Reserve, Fujian Province, China

Cited by 24 publications

References 35 publications

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Zanthoxylum bungeanum root-rot associated shifts in microbiomes of root endosphere, rhizosphere, and soil

The influence of heavy metals on biological soil quality assessments in the Vaccinium myrtillus L. rhizosphere under different field conditions

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