Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect

Lang, Ming; Bei, Shuikuan; Li, Xia; Kuyper, Thomas W.; Zhang, Junling

doi:10.3389/fmicb.2019.02856

Cited by 20 publications

(14 citation statements)

References 67 publications

(75 reference statements)

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“…For rice, there is a gradient of host‐driven microbiome filtering, with the host influence being lowest in the rhizosphere, followed by the rhizoplane, and endosphere (root interior) (Edwards et al, 2015). Similar filtering patterns with decreasing host influence with further distance to the root surface have been observed for other plants (Lang et al, 2019; Tkacz et al, 2020; Zhong et al, 2022). However, these studies either homogenized roots or did not clearly state that roots were sorted before microbial analyses, so it is unclear whether host‐driven filtering is consistent across fine root functional types.…”

Section: Discussionsupporting

confidence: 80%

Functionally discrete fine roots differ in microbial assembly, microbial functional potential, and produced metabolites

King,

Yates,

Cao

et al. 2023

Plant Cell & Environment

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Traditionally, fine roots were grouped using arbitrary size categories, rarely capturing the heterogeneity in physiology, morphology and functionality among different fine root orders. Fine roots with different functional roles are rarely separated in microbiome‐focused studies and may result in confounding microbial signals and host‐filtering across different root microbiome compartments. Using a 26‐year‐old common garden, we sampled fine roots from four temperate tree species that varied in root morphology and sorted them into absorptive and transportive fine roots. The rhizoplane and rhizosphere were characterized using 16S rRNA gene and internal transcribed spacer region amplicon sequencing and shotgun metagenomics for the rhizoplane to identify potential microbial functions. Fine roots were subject to metabolomics to spatially characterize resource availability. Both fungi and bacteria differed according to root functional type. We observed additional differences between the bacterial rhizoplane and rhizosphere compartments for absorptive but not transportive fine roots. Rhizoplane bacteria, as well as the root metabolome and potential microbial functions, differed between absorptive and transportive fine roots, but not the rhizosphere bacteria. Functional differences were driven by sugar transport, peptidases and urea transport. Our data highlights the importance of root function when examining root‐microbial relationships, emphasizing different host selective pressures imparted on different root microbiome compartments.

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Section: Discussionsupporting

confidence: 80%

Functionally discrete fine roots differ in microbial assembly, microbial functional potential, and produced metabolites

King,

Yates,

Cao

et al. 2023

Plant Cell & Environment

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“…The soil parameters were as follows: pH, 8.535; salt content, 0.2143%; electrical conductivity (EC), 622 μ s/cm; organic matter, 25.847 g/kg; total N, 1.67 g/kg; and Olsen-P, 13.42 g/kg. Briefly, to isolate the bacterium, roots (1 g fresh weight) were thoroughly washed, homogenized in 0.5 × phosphate-buffered saline (PBS) (15 mL) [ 11 ], serially diluted to 10 −7 in sterile nutrient agar (NA) medium with a 4% NaCl concentration, and placed in a 30°C incubator for 48–72 h [ 12 ]. The bacterium was subcultured twice.…”

Section: Methodsmentioning

confidence: 99%

Bacillus subtilis HG-15, a Halotolerant Rhizoplane Bacterium, Promotes Growth and Salinity Tolerance in Wheat (Triticum aestivum)

Tian

Wang

et al. 2022

BioMed Research International

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Certain plant growth-promoting bacteria (PGPB) reduce salt stress damage in plants. Bacillus subtilis HG-15 is a halotolerant bacterium (able to withstand NaCl concentrations as high as 30%) isolated from the wheat rhizoplane in the Yellow River delta. A qualitative and quantitative investigation of the plant growth-promoting characteristics of this strain confirmed nitrogen fixation, potassium dissolution, ammonia, plant hormone, ACC deaminase, and proline production abilities. B. subtilis HG-15 colonization of wheat roots, stems, and leaves was examined via scanning electron microscopy, rep-PCR, and double antibiotic screening. After inoculation with the B. subtilis HG-15 strain, the pH (1.08–2.69%), electrical conductivity (3.17–11.48%), and Na+ (12.98–15.55%) concentrations of rhizosphere soil significantly decreased ( p < 0.05 ). Under no-salt stress (0.15% NaCl), low-salt stress (0.25% NaCl), and high-salt stress (0.35% NaCl) conditions, this strain also significantly increased ( p < 0.05 ) the dry weight (17.76%, 24.46%, and 9.31%), fresh weight (12.80%, 20.48%, and 7.43%), plant height (7.79%, 5.86%, and 13.13%), and root length (10.28%, 17.87%, and 48.95%). Our results indicated that B. subtilis HG-15 can effectively improve the growth of wheat and elicit induced systemic tolerance in these plants, thus showing its potential as a microbial inoculant that can protect wheat under salt stress conditions.

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“…On this basis, further research on this topic could provide valuable information about the mobility of P cycling functions within bacterial communities. Functional prediction for enriched rhizospheric bacterial taxa was performed for clover and maize under contrasting P availabilities, finding significant differences between the two plant species in terms of genes that are relevant for P metabolism and carbon cycling (Lang et al, 2019).…”

Section: Rhizospheric Microbial Communities As Affected By Variationsmentioning

confidence: 99%

Phosphobacteria as key actors to overcome phosphorus deficiency in plants

Castagno

Sannazzaro

González

et al. 2021

Annals of Applied Biology

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Phosphorus (P), an essential macronutrient for all living organisms, is required in large amounts for the growth and development of plants. Although total soil P level is high, P bioavailability to plants is suboptimal in most soils because of high fixation rates into inorganic and organic insoluble complexes. Hence, plants are highly dependent on mechanisms that allow them to adapt to low‐phosphate stress and/or achieve suitable levels of soluble P on the root surface. In this regard, the rhizospheric microbiota plays a key role in facilitating P nutrition and has long been recognised for their potential use as an environmental‐friendly alternative to chemical P fertilisation. Herein, we outline the advances in the identification of phosphate solubilising bacteria and phosphate mineralising bacteria, collectively known as phosphobacteria, and their role in rendering P accessible to plants. We review and discuss research progress related to the introduction of phosphobacteria and/or P‐mineralising enzymes into soil, as well as plant transformation with bacterial genes that encode such enzymes, as strategies to improve plant growth and P nutrition. We also provide an overview of studies about the impact of variations in soil P levels on the structure of soil and rhizospheric microbial communities and the potential consequences of such perturbations on plant growth. Finally, we discuss possible directions for future research to optimise the efficiency of biofertilisation strategies based on the use of phosphobacteria.

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Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect

Cited by 20 publications

References 67 publications

Functionally discrete fine roots differ in microbial assembly, microbial functional potential, and produced metabolites

Functionally discrete fine roots differ in microbial assembly, microbial functional potential, and produced metabolites

Bacillus subtilis HG-15, a Halotolerant Rhizoplane Bacterium, Promotes Growth and Salinity Tolerance in Wheat (Triticum aestivum)

Phosphobacteria as key actors to overcome phosphorus deficiency in plants

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