Rhizogenesis and somatic embryogenesis in calli from wild olive (Olea europaea var. sylvestris (Miller) Lehr) mature zygotic embryos

Orinos, Th.; Mitrakos, K.

doi:10.1007/bf00041288

Cited by 50 publications

(34 citation statements)

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“…Interestingly, the formation of roots were observed in both varieties, all types of explant, on all media as well as in the dark and on the light. That confi rms statement of Orinos and Mitrakos (1991) that rhizogenesis is a labile morphogenic reaction in the sense that it is infl uenced by various factors tested. In our experiment during the whole cultivation period rhizogenesis was the strongest on the medium with 2iP.…”

Section: Discussionsupporting

confidence: 65%

“…In our experiment during the whole cultivation period rhizogenesis was the strongest on the medium with 2iP. In contrast this cytokinin can inhibit root formation in higher concentration (Orinos and Mitrakos, 1991). The frequent and spontaneous rhizogenesis on media with diff erent combinations of growth regulators and in diff erent grass pea genotypes was also mentioned by Sinha et al (1983).…”

Section: Discussionsupporting

confidence: 53%

See 1 more Smart Citation

Callus Induction and Rhizogenesis in Lathyrus sativus L.

Piwowarczyk¹,

Pindel²,

Muszyńska³

2016

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Grass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. However, neglected by breeders, it needs the improvement of some disadvantages. Biotechnological techniques, including in vitro culture, are new and modern tools supporting plant breeding. Unfortunately, grass pea belongs to grain legumes that are well-known for their recalcitrance to in vitro manipulation and consequently plant regeneration. The aim of the study was the evaluation of diff erent factors infl uence on callus induction and proliferation, as well as cell diff erentiation and organogenesis. Callus culture were initiated from diff erent explants of two Polish cultivars of grasspea on media supplemented with auxin and various cytokinins both on the light and in the dark. The rate of tissue proliferation was signifi cantly increased in both tested cultivars by light. More than 400 mg of tissue from one explant was obtained a er 12 weeks of culture. The most intensive increase in callus mass was noted for internode fragments of 'Krab' (380 mg/one explant) and root fragments of 'Derek' (361 mg/one explant). On the media with the addition of thidiazuron callus tissue grew better (852 mg/one explants) than on the media with zeatin and kinetin (56-164 mg/one explant). Formation of the roots was the only type of organogenesis observed during the study. In 'Derek' callus on LP medium rhizogenesis occurred the most frequently (38%). The roots regenerated from this callus was also the most numerous (1.6) and the longest (12.5 mm).

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Section: Discussionsupporting

confidence: 65%

Section: Discussionsupporting

confidence: 53%

Callus Induction and Rhizogenesis in Lathyrus sativus L.

Piwowarczyk¹,

Pindel²,

Muszyńska³

2016

Acta Univ. Agric. Silvic. Mendelianae Brun.

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“…(e.g. Rugini 1988;Orinos and Mitrakos 1991;Mitrakos et al 1992;Leva et al 1995;Rugini and Caricato 1995;Shibli et al 2001; Benelli et al described an efficient true-to-type micropropagation (by stem cuttings) protocol for O. europaea, which was confirmed by RAPD analysis. The analyses of genetic variability/stability during the somatic embryogenesis process (or other micropropagation processes) may be a helpful tool to predict if any detected genetic instability could be initiated at an early stage of callus formation/proliferation and thus contribute to the understanding of different rates of genetic instability among genotypes.…”

Section: Resultsmentioning

confidence: 98%

“…In O. europaea, somatic embryogenesis has been induced mostly in juvenile material from cotyledonary tissues (Orinos and Mitrakos 1991;Leva et al 1995;Trabelsi et al 2003), immature zygotic embryos (Rugini 1988;Leva et al 1995) and mature embryo callus (Orinos and Mitrakos 1991;Mitrakos et al 1992). Only Rugini and Caricato (1995) succeeded in inducing SE from leaf petiole tissues of adult plants using a method which they designated 'double regenerating system' and which requires previous rejuvenation by in vitro shoot culture.…”

Section: Introductionmentioning

confidence: 98%

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

Lopes

Capelo

Brito

et al. 2008

Trees

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The crop species Olea europaea L. (olive tree) is of great economic importance in the Mediterranean region. Hence, many efforts have been done in the last decades to propagate this commercially valuable species by in vitro methods. On the other hand, the lesser known Olea maderensis (Lowe) Rivas Mart. & Del Arco which is a native species of the Madeira Archipelago has only been the subject of micropropagation from nodal stem cuttings. Therefore, in this work we analysed the stability of ten nuclear simple sequence repeat (SSR) markers at successive stages of the somatic embryogenesis process in two adult trees belonging to these two species from the Madeira Archipelago. For the induction of somatic embryogenesis, petiole and leaf explants were cultivated on solid Murashige and Skoog medium (MS) with 12.25 lM indole-3-butyric acid (IBA) and 4.56 lM of zeatin, in the dark. After 3 months, different callus tissues (non-embryogenic, pre-embryogenic and embryogenic) developed from leaf explants and petioles were later transferred to MS medium without growth regulators in the dark. All ten SSR markers were able to distinguish between species. However, no mutations were found at the SSR loci at any of the successive developmental stages from PEMs (pre-embryogenic masses) to somatic embryos. This genetic uniformity was observed within material derived from each genotype/species and its respective donor plant. Therefore, we conclude that the genomic integrities of both O. europaea and O. maderensis were maintained throughout the stages of the embryogenic processes in study suggesting the absence of somaclonal variation.

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“…Chalkidikis. Orinos and Mitrakos (1991) observed the highest embryogenic capacity in calli derived from radicle explants of mature zygotic embryos of wild olive (O. europaea var. sylvestris) after 21 days of culture on inductive medium with high concentration of auxin.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Olive

Rugini

Gutiérrez-Pesce

Muleo

2008

Compendium of Transgenic Crop Plants

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Biochemical and molecular procedures have been developed for olive genotyping, for taxonomy between cultivated and wild olives, for phylogenetic studies of cultivars, and for identification of usable markers linked to the most important agronomic traits, such as size of the tree, flowering induction, apical dominance, productivity, self‐fertility, quantity and quality of oil and secondary products of fruit, biotic and abiotic resistance. These characters could be introgressed into cultivars by conventional breeding or through transgeny. Successful results by classical breeding are difficult to obtain due to the different flower fertility and the long juvenile period of the progenies. The low efficiency of modern genetic improvement techniques suggests that genetic transformation techniques could speed up the development of new genotypes; however, the development of efficient regeneration methods from mature tissue is the limiting factor for applying this technology, in olive. Transgenic olive plants with modified growth habit and putative induced disease resistance are under filed conditions to test their phenotype. Other attempts of genetic improvement such as clonal selection, mutation through γ‐irradiation, protoplast technology, haploid, changes in ploidy level, somaclonal variation, and somatic hybridization are discussed and reviewed.

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Rhizogenesis and somatic embryogenesis in calli from wild olive (Olea europaea var. sylvestris (Miller) Lehr) mature zygotic embryos

Cited by 50 publications

References 4 publications

Callus Induction and Rhizogenesis in Lathyrus sativus L.

Callus Induction and Rhizogenesis in Lathyrus sativus L.

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

Olive

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