Rhizobium subbaraonis sp. nov., an endolithic bacterium isolated from beach sand

Ramana, Ch. V.; Parag, Bhavyata; Girija, K. R.; Ram, Bhagwana; Ramana, V. Venkata; Sasikala, Ch.

doi:10.1099/ijs.0.041442-0

Cited by 42 publications

(13 citation statements)

References 37 publications

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“…Members of the genus Rhizobium are characterized as Gram-stain-negative, non-spore-forming, rod-shaped, aerobic, chemo-organotrophic, and possess C 18:1 and C 18:1 ω 7c as the predominant fatty acid and have a DNA G+C content of between 55 and 66 mol% [5,6]. Non-symbiotic and free-living members of Rhizobium have been found in various soils, including the rhizosphere [7,8], bioreactor [9], and beach sand [10].…”

Section: Introductionmentioning

confidence: 99%

Rhizobium desertarenaesp. nov., isolated from the Saline Desert Soil from the Rann of Kachchh, India

Khairnar

Hagir

Narayan

et al. 2020

Preprint

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AbstractA novel bacterial strain designated ADMK78T was isolated from the saline desert soil. The cells were rod-shaped, Gram-negative, and non-motile. The strain ADMK78T grows best at 28°C and pH 7.0 and can tolerate up to 2% (w/v) NaCl. Based on 16S rRNA gene phylogeny, the strain ADMK78T belongs to the genus Rhizobium, with the highest similarity to Rhizobium wuzhouense W44T (98.7%) and Rhizobium ipomoeae shin9-1T (97.9%). Core-genes based phylogenetic analysis revealed that the strain ADMK78T forms a distinct branch in between Rhizobium ipomoeae shin9-1T and Rhizobium selenitireducens BAA-1503T. The average nucleotide identity of ADMK78T was less than 82%, to members of the family Rhizobiaceae. The genomic DNA G+C content of strain ADMK78T is 58.6 mol%. The major fatty acids of strain ADMK78T were C18:0 and C18:1 ω7c. The strain ADMK78T showed differences in physiological, phenotypic, and protein profiles estimated by MALDI-TOF MS to its closest relatives. Based on the phenotypic, chemotaxonomic properties, and phylogenetic analyses, the strain ADMK78T could be distinguished from the recognized species of the genus Rhizobium. It is suggested to represent a novel species of this genus, for which the name Rhizobium desertarenae sp. nov. is proposed. The type strain is ADMK78T (=MCC 3400T; KACC 21383T; JCM 33657T).

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Section: Introductionmentioning

confidence: 99%

Rhizobium desertarenaesp. nov., isolated from the Saline Desert Soil from the Rann of Kachchh, India

Khairnar

Hagir

Narayan

et al. 2020

Preprint

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“…It is well established that in addition to being symbiotically associated with legumes, a rather large population of non-symbiotic rhizobia are found in the bulk soil, the rhizospheres of legumes and other plants (Chaintreuil et al , 2000; Peng et al , 2008; Berge et al , 2009; Zhang et al , 2011). Rhizobia have also been isolated from infertile or polluted environments such as beach sand, wastewater and oil-contaminated soil (Ramana et al , 2013; Yan et al , 2010; Zhang et al , 2012). Apart from their capacity for nitrogen fixation, species of rhizobia can also promote the growth of different plants including leguminous and nonleguminous plants, and weather silicate minerals (Tan et al , 2001; Yanni et al , 1997; Zhao et al , 2013).…”

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Rhizobium yantingense sp. nov., a mineral-weathering bacterium

Chen

Sheng

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, rod-shaped bacterial strain, H66(T), was isolated from the surfaces of weathered rock (purple siltstone) found in Yanting, Sichuan Province, PR China. Cells of strain H66(T) were motile with peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain H66(T) belongs to the genus Rhizobium. It is closely related to Rhizobium huautlense SO2(T) (98.1 %), Rhizobium alkalisoli CCBAU 01393(T) (98.0 %) and Rhizobium cellulosilyticum ALA10B2(T) (98.0 %). Analysis of the housekeeping genes, recA, glnII and atpD, showed low levels of sequence similarity (<92.0 %) between strain H66(T) and other recognized species of the genus Rhizobium. The predominant components of the cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The G+C content of strain H66(T) was 60.3 mol%. Strain H66(T) is suggested to be a novel species of the genus Rhizobium based on the low levels of DNA-DNA relatedness (ranging from 14.3 % to 40.0 %) with type strains of species of the genus Rhizobium and on its unique phenotypic characteristics. The namehttp://dx.doi.org/10.1601/nm.1279Rhizobium yantingense sp. nov. is proposed for this novel species. The type strain is H66(T) ( = CCTCC AB 2014007(T) = LMG 28229(T)).

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“…However, the manuscript describing T. marinus has no discussion of the genus Barrientosiimonas (Lee, 2013). While working on endolithic bacteria (Parag et al , 2013; Ramana et al , 2013), we isolated strain JC268 T from pebbles, and its 16S rRNA gene sequence showed highest similarity (>97 %) with Barrientosiimonas humi 39 T and T. marinus MSW-24 T . Based on polyphasic taxonomic analysis, we propose strain JC268 T to be a representative of a novel species of the genus Barrientosiimonas and to reclassify T. marinus as a member of this genus.…”

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confidence: 99%

“…The 16S rRNA gene sequence of the isolates was obtained by colony PCR and subsequent sequencing as described previously (Ramana et al , 2013) with the reverse primer R′-1525 (5′-AGAAAGGAGGTGATCCAGCC-3′) instead of R′1498. Identification of phylogenetic neighbours and calculation of pairwise 16S rRNA gene sequence similarity were achieved using the EzTaxon-e server (Kim et al , 2012).…”

mentioning

confidence: 99%

Barrientosiimonas endolithica sp. nov., isolated from pebbles, reclassification of the only species of the genus Tamlicoccus, Tamlicoccus marinus Lee 2013, as Barrientosiimonas marina comb. nov. and emended description of the genus Barrientosiimonas

Parag

Sasikala²,

Ramana

2015

International Journal of Systematic and Evolutionary Microbiology

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Strain JC268T was isolated from pebbles collected from a dam located in Lalitpur, Uttar Pradesh, India. Cells of strain JC268T were coccoid, appeared in pairs/triads/tetrads or short chains and were Gram-stain-positive, non-spore-forming, non-motile and obligately aerobic. Strain JC268T was catalase- and oxidase-positive and utilized citrate for growth. The genomic DNA G+C content of strain JC268T was 65.3 mol%. The cell-wall peptidoglycan contained l-lysine–l-serine–d-aspartic acid as interpeptide bridge with the type A4α. The major menaquinone was MK-8(H4). Major (>10 %) fatty acids were iso-C16 : 0, iso-C16 : 1H and anteiso-C17 : 1ω9c. Diphosphatidylglycerol, phosphoglycolipid, phosphatidylinositol, glycolipid, four unidentified lipids, an amino lipid and phospholipid were the polar lipids of strain JC268T. EzTaxon-e blast search of 16S rRNA gene sequences showed that strain JC268T has highest similarity to Barrientosiimonas humi 39T (98.65 %) and Tamlicoccus marinus MSW-24T (97.8 %) of the family Dermacoccaceae. Genome reassociation (based on DNA–DNA hybridization) of strain JC268T with Barrientosiimonas humi CGMCC 4.6864T ( = 39T) and T. marinus KCTC 19485T ( = MSW-24T) yielded values of 32.5 ± 2 % and 27.3 ± 2 %, respectively. Based on the data from phylogenetic and polyphasic taxonomic analyses, strain JC268T represents a novel species of the genus Barrientosiimonas for which the name Barrientosiimonas endolithica sp. nov., is proposed. The type strain of Barrientosiimonas endolithica is JC268T ( = KCTC 29672T = NBRC 110608T). Our data suggest that T. marinus should be reclassified within the genus Barrientosiimonas. Thus, a reclassification is proposed for T. marinus, the type and only species of the genus Tamlicoccus, as Barrientosiimonas marina comb. nov., which implies the emendation of the description of the genus Barrientosiimonas.

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Rhizobium subbaraonis sp. nov., an endolithic bacterium isolated from beach sand

Abstract: Rhizobium subbaraonis sp. nov., an endolithic bacterium isolated from beach sand

Cited by 42 publications

References 37 publications

Rhizobium desertarenaesp. nov., isolated from the Saline Desert Soil from the Rann of Kachchh, India

Rhizobium desertarenaesp. nov., isolated from the Saline Desert Soil from the Rann of Kachchh, India

Rhizobium yantingense sp. nov., a mineral-weathering bacterium

Barrientosiimonas endolithica sp. nov., isolated from pebbles, reclassification of the only species of the genus Tamlicoccus, Tamlicoccus marinus Lee 2013, as Barrientosiimonas marina comb. nov. and emended description of the genus Barrientosiimonas

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