Rhizobium pseudoryzae sp. nov., isolated from the rhizosphere of rice

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain J3-A127 T , was isolated from the roots of fresh rice plants (Oryza sativa). Cells were non-motile and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus Rhizobium, with closest similarity to Rhizobium oryzae Alt 505 T (96.4 %). The low levels of 16S rRNA gene sequence similarity (,90 %) found between the gyrB, atpD, recA and glnII gene sequences of strain J3-A127 T and the type strains of recognized species of the genus Rhizobium also indicated that it represented a separate species. The temperature range for growth was 10-40 6C (optimum around 28 6C) and the pH range was 6.0-11.0 (optimum pH 7.0-8.0). Strain J3-A127 T tolerated NaCl concentrations up to 5.0 % (w/v). The strain was catalase-and oxidase-positive. The main cellular fatty acids were summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6; 46.7 %). The DNA G+C content of strain J3-A127 T was 59.5 mol%. Strain J3-A127 T did not form any nodules on four different legumes and the nodD and nifH genes were not detected by PCR. According to physiological and biochemical characteristics and genotypic data, strain J3-A127 T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pseudoryzae sp. nov. is proposed. The type strain is

Novosphingobium oryzae sp. nov., a potential plant-promoting endophytic bacterium isolated from rice roots

“…Root tissue (5 g) was used to isolate the endophytes according to the previously described methods of Zhang et al (2011). Routine cultivation of strain ZYY112 T was performed with R2A agar (Difco) or trypticase soy agar (TSA; Difco) medium adjusted to pH 7.0 at 28 8C.…”

Rhizobium phenanthrenilyticum sp. nov., a novel phenanthrene-degrading bacterium isolated from a petroleum residue treatment system

“…In addition, some type strains of the genus Rhizobium were also negative for nodulation in recent studies. Rhizobium pseudoryzae J3-A127 T , isolated from the roots of rice plants Oryza sativa, did not form any nodules on four different legumes, and the nodD and nifH genes were not detected by PCR (Zhang et al, 2011). Rhizobium soli DS-42 T neither forms nodules nor is confi rmed to have the nodD and nifH genes (Yoon et al, 2010).…”

“…Strain F11 T had a similar cellular fatty acid profi le to its closest neighbors, Rhizobium oryzae Alt 505 T and Rhizobium pseudoryzae J34A-127 T ( Table 2). The major fatty acid of F11 T was C 16:0 (6.24%) and summed feature 8 (C 18:1ω7c and/or C 18:1ω6c , 76.59%), which are typical fatty acids of root-nodule bacteria (Tighe et al, 2000;Zhang et al, 2011).…”

“…In recent years, some type strains of the genus Rhizobium were found to be capable of degrading various pollutants. Rhizobium borbori DN316 T was found to degrade aniline (Zhang et al, 2011), Rhizobium selenireducens LMG 24075 T to reduce selenate to elemental red selenium (Se 0 ) (Hunter et al, 2007), Rhizobium daejeonense L61 T to degrade cyanide (Quan et al, 2005), and Rhizobium cellulosilyticum ALA10B2 T to hydrolyze polysaccharide (Paula et al, 2007).…”

Rhizobium paknamense sp. nov., isolated from lesser duckweeds (Lemna aequinoctialis)

“…Aquatic leguminous plants have also been reported to harbour species of the genus Rhizobium (Zurdo-Piñeiro et al, 2004). Previous studies showed that novel species of the genus Rhizobium can also be found as endophytes colonizing roots of nonleguminous plants including rice, wheat and maize (Peng et al, 2008;Rosenblueth & Martínez-Romero, 2004;Schloter et al, 1997;Zhang et al, 2011b). Their role in promoting growth of non-leguminous plants has also been proposed (Yanni et al, 1997).…”