Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules

Gu, Chun Tao; Wang, En Tao; Tian, Chang; Han, Tian Xu; Chen, Wen Feng; Sui, Xin; Chen, Wen Xin

doi:10.1099/ijs.0.65661-0

Cited by 44 publications

(7 citation statements)

References 13 publications

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“…Notably, Mesorhizobium huakuii was the dominant species in four rhizosphere soil samples (DX3, JX1, JX2 and PY1) in Jiangxi, south China, but M. huakuii has never been observed to nodulate soybean and is a specific microsymbiont of the green manure legume Astragalus sinicus in this eco‐region. Similarly, Rhizobium miluonense, documented as an effective microsymbiont of Lespedeza chinensis in south China, was found as the most abundant species in soybean rhizosphere at the JX3 site in the same eco‐region (Gu et al ., ). Three previously described species complexes (above the intra‐species or inter‐species boundary; Zhang et al ., ) S. meliloti/S.…”

Section: Discussionmentioning

confidence: 97%

Pyrosequencing of rpoB uncovers a significant biogeographical pattern of rhizobial species in soybean rhizosphere

Zhang

Guo

Jiao

et al. 2016

Journal of Biogeography

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Aim Most culture‐independent studies of bacterial biogeography have been at genus or higher taxonomic levels, although many important processes mediated by bacteria are at the strain or species level, such as the competitive nodulation of rhizobia on legumes. Here, at the intra‐species level, we characterized the structural variation in rhizobial populations in soybean rhizosphere under field conditions across 32 sampling sites in three eco‐regions. Location North‐east (Heilongjiang), north (Hebei–Shandong) and south (Jiangxi) China. Methods The intra‐species diversity of rhizobia in soybean rhizospheres was investigated by rpoB pyrosequencing. Soybean nodule isolates were characterized using repetitive extragenic palindromic PCR, restriction fragment length polymorphism of 16S‐23S intergenic sequences and rpoB PCR sequencing. Patterns and processes of biogeography of identified rhizobia were studied. Results Significant biogeographical patterns of rhizosphere rhizobia at the species level were found, and the abundant species in the rhizosphere were generally the dominant microsymbionts in soybean nodules. The distribution of rhizobial species in the rhizosphere across eco‐regions correlated with combined effects of spatial, vegetative, edaphic and climatic variables. Gene flow and genetic differentiation analyses revealed that there was no dispersal limitation among geographical populations of the same rhizobial species, and drift or subsequent diversification might be involved in shaping the biogeographical pattern in addition to environmental factors. Main conclusions These results constitute the first report of the existence of rhizobial biogeography in legume rhizospheres at the species level, contrasting with most studies, which focused on nodule isolates. The method developed in this study could be used to uncover, at high resolution, the diversity of local rhizobial populations in soils. This information is crucial for choosing suitable strains or species as inoculants for a specific legume host under local field conditions. Moreover, a similar strategy could be used to study other bacteria with important ecological functions.

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Section: Discussionmentioning

confidence: 97%

Pyrosequencing of rpoB uncovers a significant biogeographical pattern of rhizobial species in soybean rhizosphere

Zhang

Guo

Jiao

et al. 2016

Journal of Biogeography

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“…Rhizobium multihospitium was isolated from several legumes in China (Han et al ., ). Similarly, R. miluonense was isolated from the root nodules of Lespedeza species in China (Gu et al ., ). Highly promiscuous legumes do not require co‐introduction with compatible rhizobia from their native regions and may easily recruit novel microsymbionts in their new environments (Rodríguez‐Echeverría et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Co‐invasion of South African ecosystems by an Australian legume and its rhizobial symbionts

Ndlovu

Richardson

Wilson

et al. 2013

Journal of Biogeography

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Aim To determine and compare the taxonomic identity and diversity of root nodule and rhizospheric microbial symbionts associated with Acacia pycnantha Benth. in its native (Australian) and invasive (South African) ranges, and to establish whether these associations are general or host specific. Location The native range of A. pycnantha in Australia and invasive ranges in South Africa and Western Australia. Methods Bacteria were isolated from root nodules collected from 18 populations of A. pycnantha. Repetitive element polymerase chain reaction (REP‐PCR) fingerprinting was used to assess overall bacterial diversity and clustering. Molecular phylogenies for a subset of isolates representing major REP‐PCR clades were reconstructed using maximum parsimony and Bayesian phylogenetic analyses of the nuclear 16S–23S rRNA intergenic spacer (IGS), 16S rRNA, and the symbiotic nodA genes. Results Twelve clusters were identified from the REP‐PCR analysis; 11 included isolates from both the native range in Australia and invasive range in South Africa, while one cluster comprised only Australian isolates. Six rhizobial species were found in association with A. pycnantha: Bradyrhizobium japonicum, Rhizobium gallicum, R. lusitanum, R. miluonense, R. multihospitium and R. tropici. We also identified three plant‐growth promoting bacteria isolated from root nodules of A. pycnantha: Burkholderia caledonica, B. graminis and B. phytofirmans. Phylogenetic analysis of the IGS gene retrieved clades containing symbionts from both Australia and South Africa while others comprised only South African taxa, suggesting the introduction of bacterial lineages from Australia to South Africa. Our phylogeographic analysis of the nodA gene confirmed that A. pycnantha was co‐introduced with its symbionts to South Africa. Main conclusions Acacia pycnantha is a promiscuous legume, associated with at least six different rhizobial symbionts, and forms associations with plant‐growth promoting rhizosphere bacteria from the genus Burkholderia. In the invasive range of A. pycnantha in South Africa, nodules contained some symbionts of South African origin while other symbionts appear to have been co‐introduced from Australia. Acacia pycnantha is associated with a wider suite of symbionts in its invasive than native range.

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“…After growing for 7 weeks under natural sunlight in a greenhouse, effective nodules were observed on roots of Phaseolus vulgaris inoculated with strain CCBAU 65647 T , while no nodules were found on Mimosa pudica , Medicago sativa , Trifolium repens or Leucaena leucocephala inoculated with strain CCBAU 65647 T or on Mimosa pudica or Indigofera spicata inoculated with strains CCBAU 65643 or CCBAU 65751. This host range differed from those of R. multihospitium and R. miluonense , which failed to nodulate Phaseolus vulgaris effectively (Han et al , 2008; Gu et al , 2008). The cross-nodulation tests and nodC analysis demonstrated that the test strains were not symbionts of Mimosa pudica or Indigofera spicata , but they might be nodule endophytes of these plants.…”

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confidence: 77%

Rhizobium vallis sp. nov., isolated from nodules of three leguminous species

Wang

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Four bacterial strains isolated from root nodules of Phaseolus vulgaris, Mimosa pudica and Indigofera spicata plants grown in the Yunnan province of China were identified as a lineage within the genus Rhizobium according to the analysis of 16S rRNA gene sequences, sharing most similarity with Rhizobium lusitanum P1-7 T (99.1 % sequence similarity) and Rhizobium rhizogenes IAM 13570 T (99.0 %). These strains also formed a distinctive group from the reference strains for defined species of the genus Rhizobium in a polyphasic approach, including the phylogenetic analyses of the 16S rRNA gene and housekeeping genes (recA, atpD, glnII), DNA-DNA hybridization, BOX-PCR fingerprinting, phenotypic characterization, SDS-PAGE of whole-cell proteins, and cellular fatty acid profiles. All the data obtained in this study suggested that these strains represent a novel species of the genus Rhizobium, for which the name Rhizobium vallis sp. nov. is proposed. The DNA G+C content (mol%) of this species varied between 60.9 and 61.2 (T m ). The type strain of R. vallis sp. nov. is CCBAU 65647 T (5LMG 25295 T 5HAMBI 3073 T ), which has a DNA G+C content of 60.9 mol% and forms effective nodules on Phaseolus vulgaris.Rhizobia are symbiotic nitrogen-fixing bacteria that reduce N 2 to ammonia after forming root and/or stem nodules with leguminous plants. To date, about 80 rhizobial species have been reported within the four main genera Rhizobium, Ensifer (Sinorhizobium), Mesorhizobium and Bradyrhizobium, as well as in several other genera in the classes Alphaproteobacteria and Betaproteobacteria (Wang et al., 2006). The genus Rhizobium (Frank, 1889) was the first and only rhizobial genus described for a century, before the genus Bradyrhizobium was reported (Jordan, 1982). Since then, a series of taxonomic changes have been made in the genus Rhizobium and at the time of writing it contains 47 species, including the recently described species Rhizobium soli (Yoon et al., 2010), Rhizobium borbori (Zhang et al., 2011b), Rhizobium vignae (Ren et al., 2011) and Rhizobium tubonense (Zhang et al., 2011a).In a survey of rhizobial resources in Yunnan, a tropical province of China, four strains isolated from root nodules of Phaseolus vulgaris, Mimosa pudica and Indigofera spicata were identified as a distinct lineage in the genus Rhizobium by sequence analysis of the 16S rRNA gene, which was used to screen all the isolates. In order to clarify the taxonomic position of the four strains, a polyphasic study including phylogenetic analyses of the 16S rRNA gene and housekeeping genes (recA, atpD, glnII), BOX-PCR fingerprinting, DNA-DNA hybridization, phenotypic characterization, SDS-PAGE of whole-cell proteins, and cellular fatty acid composition was performed in comparison with reference strains of defined species of the genus Rhizobium. Based on the results, these four isolates represent a different group from all defined species and, therefore, a novel species is proposed.The four novel bacterial strains and the reference strains used in this study ...

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Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules

Cited by 44 publications

References 13 publications

Pyrosequencing of rpoB uncovers a significant biogeographical pattern of rhizobial species in soybean rhizosphere

Pyrosequencing of rpoB uncovers a significant biogeographical pattern of rhizobial species in soybean rhizosphere

Co‐invasion of South African ecosystems by an Australian legume and its rhizobial symbionts

Rhizobium vallis sp. nov., isolated from nodules of three leguminous species

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