Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba

Three microbial strains isolated from common beans, 23C2T (Tunisia), Gr42 (Spain) and IE4868 (Mexico), which have been identified previously as representing a genomic group closely related to Rhizobium gallicum , are further studied here. Their 16S rRNA genes showed 98.5–99 % similarity with Rhizobium loessense CCBAU 7190BT, R. gallicum R602spT, Rhizobium mongolense USDA 1844T and Rhizobium yanglingense CCBAU 71623T. Phylogenetic analysis based on recA, atpD, dnaK and thrC sequences showed that the novel strains were closely related and could be distinguished from the four type strains of the closely related species. Strains 23C2T, Gr42 and IE4868 could be also differentiated from their closest phylogenetic neighbours by their phenotypic and physiological properties and their fatty acid contents. All three strains harboured symbiotic genes specific to biovar gallicum. Levels of DNA–DNA relatedness between strain 23C2T and the type strains of R. loessense , R. mongolense , R. gallicum and R. yanglingense ranged from 58.1 to 61.5 %. The DNA G+C content of the genomic DNA of strain 23C2T was 59.52 %. On the basis of these data, strains 23C2T, Gr42 and IE4868 were considered to represent a novel species of the genus Rhizobium for which the name Rhizobium azibense is proposed. Strain 23C2T ( = CCBAU 101087T = HAMBI3541T) was designated as the type strain.

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“…T was 59.52 mol% which is within the range reported for other species of the genus Rhizobium (Ramírez-Bahena et al, 2008;Tian et al, 2008).…”

supporting

confidence: 84%

Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris

Mnasri

Liu

Saïdi

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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“…Each seed was inoculated with 1 mL of a log phase rhizobial culture (10 9 cells mL −1 ). Growth conditions of faba bean plants were [12][13][14][15][16][17][18][19][20][21][22][23][24][25] • C (night/day), a relative humidity of 50%-60%, and a photoperiod of 10 hr. At flowering stage, 50 days after sowing, plants were uprooted and assayed for dry weight of nodules, shoots and roots dry weight, as well as shoot N-uptake by faba bean plants.…”

Section: Symbiotic Effectiveness Under Greenhouse Conditionsmentioning

confidence: 99%

“…viciae (Rlv) [16]. Later, R. fabae [17], R. laguerrereae [18], R. etli [19,20], and Agrobacterium tumefaciens [20] were also identified as faba bean-nodulating microsymbionts.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Faba Bean Yield Using Rhizobium/Agrobacterium Inoculant in Low-Fertility Sandy Soil

2017

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Soil fertility is one of the major limiting factors for crop's productivity in Egypt and the world in general. Biological nitrogen fixation (BNF) has a great importance as a non-polluting and a cost-effective way to improve soil fertility through supplying N to different agricultural systems. Faba bean (Vicia faba L.) is one of the most efficient nitrogen-fixing legumes that can meet all of their N needs through BNF. Therefore, understanding the impact of rhizobial inoculation and contrasting soil rhizobia on nodulation and N 2 fixation in faba bean is crucial to optimize the crop yield, particularly under low fertility soil conditions. This study investigated the symbiotic effectiveness of 17 Rhizobium/Agrobacterium strains previously isolated from different Egyptian governorates in improving the nodulation and N 2 fixation in faba bean cv. Giza 843 under controlled greenhouse conditions. Five strains that had a high nitrogen-fixing capacity under greenhouse conditions were subsequently tested in field trials as faba bean inoculants at Ismaillia Governorate in northeast Egypt in comparison with the chemical N-fertilization treatment (96 kg N·ha −1 ). A starter N-dose (48 kg N·ha −1 ) was applied in combination with different Rhizobium inoculants. The field experiments were established at sites without a background of inoculation under low fertility sandy soil conditions over two successive winter growing seasons, 2012/2013 and 2013/2014. Under greenhouse conditions, inoculated plants produced significantly higher nodules dry weight, plant biomass, and shoot N-uptake than non-inoculated ones. In the first season (2012/2013), inoculation of field-grown faba bean showed significant improvements in seed yield (3.73-4.36 ton·ha −1 ) and seed N-yield (138-153 Kg N·ha −1 ), which were higher than the uninoculated control (48 kg N·ha −1 ) that produced 2.97 Kg·ha −1 and 95 kg N·ha −1 , respectively. Similarly, in the second season (2013/2014), inoculation significantly improved seed yield (3.16-4.68 ton·ha −1 ) and seed N-yield (98-155 Kg N·ha −1 ) relative to the uninoculated control (48 kg N·ha −1 ), which recorded 2.58 Kg·ha −1 and 80 kg N·ha −1 , respectively. Interestingly, faba bean inoculated with strain Rlv NGB-FR 126 showed significant increments in seed yield (35%-48%) and seed N-yield (34%-49%) compared to the inorganic N fertilizers treatment (96 kg N·ha −1 ) over the two cropping seasons, respectively. These results indicate that inoculation of faba bean with effective rhizobial strains can reduce the need for inorganic N fertilization to achieve higher crop yield under low fertility soil conditions.

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“…fabae and R . anhuiense [9, 10, 13, 29]. In our earlier study we found that in Sichuan, in addition to R .…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Symbiotic Efficiency of Nodulating Rhizobia Isolated from Root Nodules of Faba Bean in One Field

et al. 2016

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Thirty-one nodulating rhizobium strains were collected from root nodules of spring and winter type faba bean cultivars grown in micro ecoarea, i.e. the same field in Chengdu plain, China. The symbiotic efficiency and phylogeny of these strains were studied. Effectively nitrogen fixing strains were isolated from both winter type and spring type cultivars. Based on phylogenetic analysis of 16S rRNA gene and concatenated sequence of atpD, glnII and recA genes, the isolates were assigned as Rhizobium anhuiense and a potential new Rhizobium species. The isolates were diverse on symbiosis related gene level, carrying five, four and three variants of nifH, nodC and nodD, respectively. Strains carrying similar gene combinations were trapped by both winter and spring cultivars, disagreeing with the specificity of symbiotic genotypes to reported earlier faba bean ecotypes.

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Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba

Cited by 85 publications

References 28 publications

Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris

Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris

Improvement of Faba Bean Yield Using Rhizobium/Agrobacterium Inoculant in Low-Fertility Sandy Soil

Genetic Diversity and Symbiotic Efficiency of Nodulating Rhizobia Isolated from Root Nodules of Faba Bean in One Field

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