Rhizobial Resource Associated with Epidemic Legumes in Tibet

Hou, Bao Chao; Wang, En Tao; Liu, Ying; Jia, Rui; Chen, Wen Feng; Man, Chao Xin; Sui, Xin

doi:10.1007/s00248-008-9397-4

Cited by 40 publications

(21 citation statements)

References 35 publications

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“…Nodulation of the seven test strains on their hosts of origin has been confirmed previously (Hou et al, 2009;Lin et al, 2007;Wang et al, 2006;Zhao et al, 2008). In the present study, a cross-nodulation test was performed using the standard methods (Vincent, 1970) for seed treatment and inoculation.…”

supporting

confidence: 79%

“…As shown in Table 1, the seven strains originated from six shrubbery and herbal legume species grown in five Chinese provinces or autonomous regions covering the temperate and subtropical regions, selected from 381 strains used in four independent studies on diversity of rhizobia (Hou et al, 2009;Lin et al, 2007;Wang et al, 2006;Zhao et al, 2008). These data, as well as those in the other mentioned reports, demonstrated that the R. giardinii-related bacteria are minor groups in root nodules of various legumes in divergent regions.…”

mentioning

confidence: 92%

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Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China

Ren

Wang

Chen

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Seven Rhizobium strains associated with various legume species grown in different geographical regions of China were defined into four genomic groups related to Rhizobium giardinii, based upon ribosomal intergenic spacer RFLP, phylogenies of 16S rRNA and housekeeping (atpD, recA and glnII) genes, and DNA relatedness. Three strains in group I were classified as R. giardinii, as they showed high gene sequence similarities (.97 %) and DNA relatedness (64.3-67.5 %) to R. giardinii H152 T . Groups II, III and IV differed from all defined Rhizobium species based upon the consensus of all analyses. As group II contained two strains that originated from two distinct populations, we propose this group as a novel species, Rhizobium herbae sp. nov., with strain CCBAU 83011 T (5LMG 25718 T 5HAMBI 3117 T ) as the type strain.

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confidence: 79%

mentioning

confidence: 92%

Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China

Ren

Wang

Chen

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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“…Because of its plant-associated nature, these organisms are considered to have the natural mechanisms which can be plant beneficial also. As actinomycetes of endophytic origin can also expected to have strain-specific biosynthetic uniqueness, their bioactivity analysis is very important (Hou et al 2009). Natural products from actinomycetes have been reported to have an array of properties including antimicrobial (Candicidin, Chloramphenicol, Rifamycin and Streptomycin), antitumor (Actinomycin D, Bleomycin and Doxorubicin), and immunosuppressive effects (Rapamycin and FK 506/Fujimycin) (Arndt et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Endophytic Nocardiopsis sp. from Zingiber officinale with both antiphytopathogenic mechanisms and antibiofilm activity against clinical isolates

2017

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Novel and potential antimicrobial compounds are essential to tackle the frequently emerging multidrugresistant pathogens and also to develop environment friendly agricultural practices. In the current study, endophytic actinomycetes from rhizome of Zingiber officinale were explored in terms of its diversity and bioactive properties. Fourteen different organisms were isolated, identified and screened for activity against Pythium myriotylum and human clinical pathogens. Among these, Nocardiopsis sp. ZoA1 was found to have highest inhibition with excellent antibacterial effects compared to standard antibiotics. Remarkable antibiofilm property was also shown by the extract of ZoA1. Its antifungal activity against Pythium and other common phytopathogens was also found to be promising as confirmed by scanning electron microscopic analysis. By PCR-based sequence analysis of phz E gene, the organism was confirmed for the genetic basis of phenazine biosynthesis. Further GC-MS analysis of Nocardiopsis sp. revealed the presence of various compounds including Phenol, 2,4-bis (1,1-dimethylethyl) and trans cinnamic acid which can have significant role in the observed result. The current study is the first report on endophytic Nocardiopsis sp. from ginger with promising applications. In vivo treatment of Nocardiopsis sp. on ginger rhizome has revealed its inhibition towards the colonization of P. myriotylum which makes the study to have promises to manage the severe diseases in ginger like rhizome rot.

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“…Vassilcz., an ephemeral legume growing in grasslands at altitudes of 3700-4000 m. Six of them were identified as Sinorhizobium meliloti, and six as Rhizobium strains (Hou et al, 2009). Among the Rhizobium strains, CCBAU 85039 T and CCBAU 85027 were classified as a small group by numerical taxonomy, amplified 16S rRNA gene restriction analysis, amplified 16S-23S intergenic spacer restriction analysis and 16S rRNA gene phylogeny (Hou et al, 2009). To verify the taxonomic position of these two strains, further characterization was performed in the present study.…”

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confidence: 99%

“…In addition to the sequence from strain CCBAU 85039 T obtained previously (Hou et al, 2009), the 16S rRNA gene of strain CCBAU 85027 was sequenced in this study by using the same methods. The sequence of the 16S rRNA gene of strain CCBAU 85027 was identical to that of CCBAU 85039 T , and they formed a unique phylogenetic subbranch in the genus Rhizobium in a neighbour-joining phylogenetic tree (Fig.…”

mentioning

confidence: 99%

Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Trigonella archiducis-nicolai (Sirj.) Vassilcz.

Hou

Wang

Liu

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Trigonella archiducisnicolai (Š irj.) Vassilcz. Isolated from root nodules of Trigonella archiducis-nicolai (Š irj.) Vassilcz. grown in Tibet, China, cells of the bacterial strains CCBAU 85039 T and CCBAU 85027 were Gram-negative, aerobic, motile, non-spore-forming rods that formed colonies that were semi-translucent and opalescent on yeast extract-mannitol agar. In numerical taxonomy, SDS-PAGE analysis of whole-cell proteins and DNA-DNA hybridization, the two strains were very similar and were different from reference strains of defined Rhizobium species. In the phylogeny based on 16S rRNA gene sequences, they were most similar to Rhizobium etli CFN 42 T (98.2 % similarity) and R. leguminosarum USDA 2370 T (97.6 %). Sequence analyses of the housekeeping genes recA, atpD and glnII and the 16S-23S rRNA intergenic spacer, phenotypic characteristics and cellular fatty acid profiles strongly suggested that these two strains represented a novel species within Rhizobium. Crossnodulation tests and sequencing of nifH and nodA genes showed that these two strains were symbiotic bacteria that nodulated Trigonella archiducis-nicolai, Medicago lupulina, Medicago sativa, Melilotus officinalis, Phaseolus vulgaris and Trigonella foenum-graecum. Based on the results, the novel species Rhizobium tibeticum sp. nov. is described to accommodate the two strains. The type strain is CCBAU 85039 T (5LMG 24453 T 5CGMCC 1.7071 T ). The DNA G+C content of this strain is 59.7 mol% (T m ).The genus Rhizobium was first described by Frank (1889) to accommodate all symbiotic nitrogen-fixing bacteria associated with legumes. After a series of revisions, some earlier described species have been moved into Bradyrhizobium (Jordan, 1982), Sinorhizobium (de Lajudie et al., 1994) and Mesorhizobium (Jarvis et al., 1997), while many novel species and genera of rhizobia within the Alphaproteobacteria and Betaproteobacteria have been reported (NZ Rhizobia, 2008). The current genus Rhizobium includes about 30 species of fast-growing, acid-producing rhizobia, which can be differentiated from Sinorhizobium species on the basis of 16S rRNA gene sequence-based phylogeny. With the exception of Rhizobium cellulosilyticus (Garcia-Fraile et al., 2007), most of them can induce root nodules on a certain range of legume species.In a survey of rhizobial resources in Tibet, 12 strains of root-nodule bacteria were isolated with yeast mannitol agar (YMA) medium according to the method of Vincent (1970) from root nodules of Trigonella archiducis-nicolai (Širj.) Vassilcz., an ephemeral legume growing in grasslands at altitudes of 3700-4000 m. Six of them were identified as Sinorhizobium meliloti, and six as Rhizobium strains (Hou et al., 2009). Among the Rhizobium strains, CCBAU 85039 T and CCBAU 85027 were classified as a small group by numerical taxonomy, amplified 16S rRNA gene restriction analysis, amplified 16S-23S intergenic spacer restriction analysis and 16S rRNA gene phylogeny (Hou et al., 2009). To...

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Rhizobial Resource Associated with Epidemic Legumes in Tibet

Cited by 40 publications

References 35 publications

Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China

Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China

Endophytic Nocardiopsis sp. from Zingiber officinale with both antiphytopathogenic mechanisms and antibiofilm activity against clinical isolates

Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Trigonella archiducis-nicolai (Sirj.) Vassilcz.

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