Rhizobacterium Arthrobacter agilis UMCV2 increases organ-specific expression of FRO genes in conjunction with genes associated with the systemic resistance pathways of Medicago truncatula

Montejano-Ramírez, Vicente; Martínez-Cámara, Ramiro; García-Pineda, Ernesto; Valencia-Cantero, Eduardo

doi:10.1007/s11738-018-2712-x

Cited by 8 publications

(12 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that when the soil pH increases by 1.0, the solubility of iron decreases 1000 times (Zhou et al, 2018). Previous researchers used MS medium to remove iron salt when studying the effect of microbial volatiles on the growth of A. thaliana under iron deficiency (Castulo-Rubio et al, 2015;Wang et al, 2017;Montejano-Ramirez et al, 2018), but in this experiment, the total nutrient MS medium pH was directly adjusted to 8.0. The growth environment of A. thaliana was relatively close to environmentally relevant alkaline soil conditions; that is, the soil was not free of iron, but the high-pH environment led to a decrease in iron availability (Pii et al, 2015;Aras et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…In strategy I, iron uptake by plants consists of three steps: (1) release of protons into the plant rhizosphere to reduce pH and increase the solubility of insoluble iron oxide by H + ATPase (AHA2), (2) reduction of Fe 3+ to ferrous form by plasma membrane-bound chelating iron reductase (FRO2), and (3) transport of reduced Fe 2+ to the root epidermis through divalent metal transport protein IRT1 across the plasma membrane (Del Carmen Orozco-Mosqueda et al, 2013). FRO2 and IRT1 are finely regulated by iron deficiency-induced transcription factors (FIT1), and FIT1 plays a central role in iron homeostasis (Montejano-Ramirez et al, 2018). However, neither gramineous nor non-gramineous monocotyledons can obtain enough iron in alkaline or calcareous soils by strategy I or strategy II (Castulo-Rubio et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Iron Uptake in Plants by Volatile Emissions of Rahnella aquatilis JZ-GX1

Kong

Wang

2021

Front. Plant Sci.

View full text Add to dashboard Cite

Iron deficiency in soil has crucially restricted agricultural and forestry production. Volatile organic compounds (VOCs) produced by beneficial microorganisms have been proven to play an important role in inducing abiotic stress tolerance in plants. We investigated the effects of VOCs released by the rhizobacterium Rahnella aquatilis JZ-GX1 on the growth and root parameters of Arabidopsis thaliana under iron deficiency. The effect of the rhizobacterial VOCs on the gene expression in iron uptake and hormone signaling pathways were detected by RT-qPCR. Finally, the VOCs of the JZ-GX1 strain that could promote plant growth under iron deficiency stress were screened. The results showed that the JZ-GX1 strain could induce A. thaliana tolerance to iron deficiency stress by promoting the development of lateral roots and root hairs and increasing the activities of H+ ATPase and Fe3+ reductase. In addition, the AHA2, FRO2, and IRT1 genes of A. thaliana exposed to JZ-GX1-emitted VOCs were upregulated 25-, 1. 81-, and 1.35-fold, respectively, and expression of the abscisic acid (ABA) synthesis gene NCED3 was upregulated on both the 3rd and 5th days. Organic compounds were analyzed in the headspace of JZ-GX1 cultures, 2-undecanone and 3-methyl-1-butanol were found to promote Medicago sativa and A. thaliana growth under iron-limited conditions. These results demonstrated that the VOCs of R. aquatilis JZ-GX1 have good potential in promoting iron absorption in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Iron Uptake in Plants by Volatile Emissions of Rahnella aquatilis JZ-GX1

Kong

Wang

2021

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…3d). In addition, we measured the bacterial population sizes in m1 leaves at different post-inoculation times (24,72, and 120 h); again, no significant differences were observed in the CFUs. The inoculation experiment was repeated with the half of the cells in the inoculum but no significant differences were observed in this experiment either ( Supplementary Fig.…”

Section: Detection and Quantification Of A Agilis Umcv2 In Internal mentioning

confidence: 99%

“…The bacterium has great potential as a biofertilizer, since it is capable of improving the nutritional status of plants by promoting iron acquisition processes that involve the reduction and dissolution of Fe 3+ present in the soil [21][22][23] . In addition, analyses made by gas chromatography in tandem with mass spectrometry (GC-MS) indicated that the UMCV2 strain interacts with leguminous and monocot plants through the production of volatile compounds, promoting their growth and health 16,[21][22][23][24] . A study using the DLI-ESI-MS method showed that volatiles from A. agilis UMCV2 induce the accumulation of different metabolites involved in the iron-adaptive processes in Medicago truncatula 16 .…”

mentioning

confidence: 99%

Metabolomic effects of the colonization of Medicago truncatula by the facultative endophyte Arthrobacter agilis UMCV2 in a foliar inoculation system

Ramírez-Ordorica

Valencia-Cantero

Flores-Cortez

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Biofertilizer production and application for sustainable agriculture is already a reality. The methods for biofertilizers delivery in crop fields are diverse. Although foliar spray is gaining wide acceptance, little is known about the influence that the biochemical features of leaves have on the microbial colonization. Arthrobacter agilis UMCV2 is a rhizospheric and endophytic bacteria that promotes plant growth and health. In this study, we determined the capacity of the UMCV2 strain to colonize different leaves from Medicago truncatula in a foliar inoculation system. By using two powerful analytical methods based on mass spectrometry, we determined the chemical profile of the leaves in 15-d old plants. The metabolic signatures between the unifoliate leaf (m1) and the metameric units developing above (m2 and m3) were different, and interestingly, the highest colony forming units (CFU) was found in m1. The occurrence of the endophyte strongly affects the sugar composition in m1 and m2 leaves. Our results suggest that A. agilis UMCV2 colonize the leaves under a foliar inoculation system independently of the phenological age of the leaf and it is capable of modulating the carbohydrate metabolism without affecting the rest of the metabolome. Plants live in a close relationship with different microbial communities that reside inside or outside the plant tissues. The inside colonizers are referred as endophytes (from the Greek endon-within and phyton-plant), and when they are inside the plant, they completely depend on the plant and its internal conditions for growth 1-5. A study of Arabidopsis thaliana grown under natural conditions showed that the bacterial community associated with the outside of the roots were more diverse than in the endophytic community, while the opposite pattern was observed in the leaves 6. In addition, the authors found that leaves and roots shared many species of bacterial endophytes, suggesting that some root endosphere colonizers migrate and are capable of colonizing above-ground tissues; thus the microbial traits for competition and colonization influence the richness and composition of the plant endophytes 7,8. According to the literature, the endophytes are non-pathogenic, but some of them could act as latent pathogens that can cause disease, depending on the stressful environmental circumstances 4,9. Studies of the functional capacity of beneficial endophytes have revealed their potential as promoters of plant growth and health, moreover, they are free from the environmental pressure of changing soil and climate conditions experienced by their rhizospheric counterparts, so they are an excellent candidate for biofertilizers 10,11. The delivery methods of endophytes in agriculture include seed inoculation, soil drenching, stem injection, and foliar fertilization. Currently, there is a renewed interest in a foliar-treated plants and different studies on the effect of bacterial and fungal endophytes on the growth, crop production, nutrient uptake, and defense have appeared in the literatu...

show abstract

“…It emits a pool of volatiles that promotes the growth of leguminous and monocotyledonous plants with different levels of available Fe [22,23,24]. Notably, in Medicago truncatula , the UMCV2 strain increased the expression of genes involved in Fe uptake ( MtFRO1 , MtFRO2 , MtFRO3 , MtFRO4 , and MtFRO5 ) under Fe-sufficient and -deficient conditions [25]. Nevertheless, those studies focused on elucidating the molecular mechanisms involved in the modulation of Fe acquisition responses.…”

Section: Introductionmentioning

confidence: 99%

A Mass Spectrometry-Based Study Shows that Volatiles Emitted by Arthrobacter agilis UMCV2 Increase the Content of Brassinosteroids in Medicago truncatula in Response to Iron Deficiency Stress

et al. 2019

Self Cite

View full text Add to dashboard Cite

Iron is an essential plant micronutrient. It is a component of numerous proteins and participates in cell redox reactions; iron deficiency results in a reduction in nutritional quality and crop yields. Volatiles from the rhizobacterium Arthrobacter agilis UMCV2 induce iron acquisition mechanisms in plants. However, it is not known whether microbial volatiles modulate other metabolic plant stress responses to reduce the negative effect of iron deficiency. Mass spectrometry has great potential to analyze metabolite alterations in plants exposed to biotic and abiotic factors. Direct liquid introduction-electrospray-mass spectrometry was used to study the metabolite profile in Medicago truncatula due to iron deficiency, and in response to microbial volatiles. The putatively identified compounds belonged to different classes, including pigments, terpenes, flavonoids, and brassinosteroids, which have been associated with defense responses against abiotic stress. Notably, the levels of these compounds increased in the presence of the rhizobacterium. In particular, the analysis of brassinolide by gas chromatography in tandem with mass spectrometry showed that the phytohormone increased ten times in plants grown under iron-deficient growth conditions and exposed to microbial volatiles. In this mass spectrometry-based study, we provide new evidence on the role of A. agilis UMCV2 in the modulation of certain compounds involved in stress tolerance in M. truncatula.

show abstract

Rhizobacterium Arthrobacter agilis UMCV2 increases organ-specific expression of FRO genes in conjunction with genes associated with the systemic resistance pathways of Medicago truncatula

Cited by 8 publications

References 60 publications

Enhanced Iron Uptake in Plants by Volatile Emissions of Rahnella aquatilis JZ-GX1

Enhanced Iron Uptake in Plants by Volatile Emissions of Rahnella aquatilis JZ-GX1

Metabolomic effects of the colonization of Medicago truncatula by the facultative endophyte Arthrobacter agilis UMCV2 in a foliar inoculation system

A Mass Spectrometry-Based Study Shows that Volatiles Emitted by Arthrobacter agilis UMCV2 Increase the Content of Brassinosteroids in Medicago truncatula in Response to Iron Deficiency Stress

Contact Info

Product

Resources

About