Rhizobacteria-Mediated Growth Promotion of Tomato Leads to Protection AgainstCucumber mosaic virus

Abstract

We evaluated combinations of two strains of plant growth-promoting rhizobacteria (PGPR) formulated with the carrier chitosan for the ability to induce growth promotion of tomato plants and resistance to infection by Cucumber mosaic virus (CMV). Each PGPR combination included GB03 (Bacillus subtilis) and one of the following PGPR strains: SE34 (B. pumilus), IN937a (B. amyloliquefaciens), IN937b (B. subtilis), INR7 (B. pumilus), or T4 (B. pumilus). The PGPR combinations formulated with chitosan are referred to as biopreparations. Tomato plants treated with each of the biopreparations appeared phenotypically and developmentally similar to nonbacterized control plants that were 10 days older (referred to as the older control). When plants were challenged with CMV, all plants in the biopreparation treatments and the older control treatment had significantly greater height, fresh weight, and flower and fruit numbers than that of plants in the CMV-inoculated same age control treatment. CMV disease severity ratings were significantly lower for biopreparation-treated and older control tomato plants than for that of same age control plants at 14 and 28 days postinoculation (dpi). CMV accumulation in young noninoculated leaves was significantly less for all biopreparation-treated plants and those in the older control than for the same age control plants at 14 dpi and for four of the five biopreparation treatments at 28 dpi. In those tomato plants shown to be infected, the amount of CMV in noninoculated leaves was significantly lower for three of the biopreparation treatments and the older control treatment at 14 dpi and biopreparation G/INR7 treatment at 28 dpi when compared with the control treatment. These data show that treatment of tomato plants with biopreparations results in significant enhancement of growth and protection against infection by CMV.

Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)

“…Several PGPB were formulated using chitosan as a carrier mixed with soilless growth medium for successful biological control against cucumber mosaic virus in tomato (Murphy et al 2003). Five PGPB were prepared into several formulations of polymers composed of carboxymethylcellulose starch.…”

Current Advances in the Fusarium Wilt Disease Management in Banana with Emphasis on Biological Control

“…Both of these defense mechanisms are associated with disease resistance. ISR elicited by PGPR has shown promise in managing a wide spectrum of plant pathogens in several plant species under greenhouse and field environments (Radjacommare et al, 2004;Thangavelu et al 2004;Murphy et al, 2003). Fishal et al (2010) observed increased accumulation of resistance-related enzymes such as peroxidase (PO), phenylalanine ammonia lyase (PAL), lignithioglycolic acid (LTGA), and pathogenesis-related (PR) proteins (chitinase and -1, 3-glucanase) in banana plantlets treated with endophytic bacteria UPMP3 and UPMB3 singly or as mixture under glasshouse conditions.…”

Modulation of Quorum Sensing in Acyl-homoserine Lactone-Producing or -Degrading Tobacco Plants Leads to Alteration of Induced Systemic Resistance Elicited by the Rhizobacterium Serratia marcescens 90-166

“…CMV strain Fny was provided by Peter Palukaitis (Scottish Crop Research Institute, Invergowrie, Scotland) and was used for all virus-based experiments in the current study. Leaf inoculation with CMV was performed as described previously by Ryu et al (2004b) and Murphy et al (2003). Briefly, CMV was maintained in N. tabacum cultivar Kentucky 14 by mechanical passage in a temperature-controlled greenhouse.…”