Rhizobacteria induces resistance against Fusarium wilt of tomato by increasing the activity of defense enzymes

Ferraz, Hélvio Gledson Maciel; Resende, Renata Sousa; Silveira, Patrícia Ricardino; Andrade, Camila Cristina Lage de; Milagres, Elisângela Aparecida; Oliveira, José Rogério de; Rodrigues, Fabrício Á.

doi:10.1590/1678-4499.0124

Cited by 28 publications

(15 citation statements)

References 34 publications

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“…JA is a phytohormone directly involved in the plant defense responses against stress, and used as a stress indicator. Choudhary (2011) and Ferraz et al (2014) reported that the application of biotic inducers able to stimulate LOX activity, contributed to the resistance of tomato plants against Fusarium sp., and also in soybean plants against Macrophomina phaseolina. In both cases, the disease control was attributed to the activation of JA pathway.…”

Section: Characmentioning

confidence: 99%

Lactobacillus plantarum Exopolysaccharides Induce Resistance against Tomato Bacterial Spot

Blainski¹,

Neto²,

Luiz³

et al. 2017

JAS

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Lactic acid bacteria produce several exopolysaccharides (EPS) that may have antimicrobial action and/or induce defense responses in plants. This work aims to evaluate the potential of EPS produced by Lactobacillus plantarum in the protection of tomato plants against bacterial spot caused by Xanthomonas gardneri, as well as to predict the possible mechanisms of action. The EPS were characterized through FTIR and applied at 0; 0.5; 1.5 and 3.0 mg mL -1 in tomato plants with five expanded leaves, followed by the pathogen inoculation after 3 or 7 days. Antimicrobial activity of the biopolymer (1.5 or 10.0 mg mL -1 ) was evaluated in bioassay when EPS was incorporated into culture medium or embedded in antibiogram disk. The defense mechanisms i.e., total phenolic compounds and flavonoids content, phenylalanine ammonia-lyase (PAL), glutathione reductase (GR) and lipoxygenase (LOX) activities, were measured in tomato plants treated with EPS (1.5 mg mL -1 ), inoculated or not with X. gardneri. EPS reduced bacterial spot symptoms by up to 72.0% compared to the control. There were no direct effects of EPS on the in vitro growth of X. gardneri. The spectrophotometric profile, ascorbic and ellagic acid concentrations were change in tomato plants after EPS application, in plants challenged with the pathogen. Increases in PAL, GR and LOX activities were observed in plants treated with EPS. Thus, the application of L. plantarum exopolysaccharides can be considered as an effective alternative for controlling bacterial spot in tomato plants. This paper also discusses how these exopolysaccharides reduced the severity of the disease.

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Section: Characmentioning

confidence: 99%

Lactobacillus plantarum Exopolysaccharides Induce Resistance against Tomato Bacterial Spot

Blainski¹,

Neto²,

Luiz³

et al. 2017

JAS

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“…Table 2. Analysis of variance of the effects of treatments (antagonists UFV252, UFV592 and UFV618; jasmonic acid and control) and evaluation times for peroxidase (POX), polyphenoloxidase (PPO), β-1,3-glucanase (GLU), chitinase (CHI), phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX) activities and malonic aldehyde (MDA) concentration mechanisms of PGPR revealed that several strains protect plants from pathogen attack through an increase in POX activity (Ferraz et al, 2014;Ganeshamoorthi et al, 2008;Vanitha & Umesha, 2011). This enzyme is important for the lignification of plant tissues, and lignin precursors exhibit antimicrobial activity (Ride, 1975).…”

Section: Resultsmentioning

confidence: 99%

“…The antagonists UFV252, UFV592, and UFV618 were selected from a universe of 635 bacterial isolates that promote biocontrol of Fusarium wilt (Ferraz et al, 2014), bacterial spot and early blight and in tomato plants. The antagonists were identified as Serratia marcescens (UFV252), Bacillus cereus (UFV592) and Streptomyces setonii (UFV618) by sequencing the 16S rDNA fragment, with the following numbers deposited in GenBank: KM463767, KM463766 and KM 463768, respectively.…”

Section: Source Of Xg and Antagonists Cultivation And Preservationmentioning

confidence: 99%

“…Biological control and the application of resistance inducers may be a viable strategy for crops that do not have registered pesticides or for which registered products appear to be ineffective (Ferraz et al, 2014;Lanna et al, 2013;Shanmugam & Kanoujia, 2011). In this context, the utilization of resistance induced by non-pathogenic bacteria and abiotic inducers for disease control in agronomic crops has increased in recent years (Ferraz et al, 2014;Lanna et al, 2013;Nafie et al, 2011;Van Loon, 2007). In addition, there is an increasing demand by society for healthier food and less harm to the environment.…”

Section: Introductionmentioning

confidence: 99%

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Antagonistic rhizobacteria and jasmonic acid induce resistance against tomato bacterial spot

et al. 2015

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Tomato bacterial spot on tomato may be caused by four species of Xanthomonas and among them X. gardneri (Xg) is the most destructive one, especially in areas irrigated using a center pivot system in Minas Gerais state and the midwest region of Brazil. Due to the ineffectiveness of chemical control and the lack of cultivars with high levels of genetic resistance, this study investigated the potential of three antagonists (Streptomyces setonii (UFV618), Bacillus cereus (UFV592) and Serratia marcescens (UFV252)), and the hormone jasmonic acid (JA) as a positive control, to reduce bacterial spot symptoms and to potentiate defense enzymes in the leaves of tomato plants infected by Xg. Tomato seeds were microbiolized with each antagonist, and the soil was drenched with these bacteria. The plants were sprayed with JA 48 h before Xg inoculation. The final average severity on the tomato plants was reduced by 29.44, 59.26 and 61.33% in the UFV592, UFV618 and JA treatments, respectively. The UFV618 antagonist was as effective as JA in reducing bacterial spot symptoms on tomatoes, which can be explained by the greater activities of defense enzymes that are commonly involved in host resistance against bacterial diseases. These results suggest that JA and the UFV618 antagonist can be used in the integrated management of bacterial spot on tomatoes.

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“…358 were reported to reduce Fusarium wilt on cucumber plants (Singh et al, 1999). Other study reported the potential of three antagonists, Streptomyces setonii, Bacillus cereus, and Serratia marcescens to reduce Fusarium wilt symptoms in the stem of tomato plants by F.oxysporum (Ferraz et al, 2014). Other factor besides hydrolytic enzymes produced by antagonistic bacteria in reducing Fusarium wilt were siderophores, cyanide, and antibiotics (Cantelan et al, 1999).…”

Section: In Vivo Antagonism Of Two Selected Antagonistic Bacterial Ismentioning

confidence: 99%

A Possibility of using Antagonistic Bacterial Isolates in Controlling Fusarium Wilt of Chrysanth (Chrysanthemum sp.)

Sibagariang¹,

Suryanto²,

Nurtjahja³

2019

J Pure Appl Microbiol

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One problem faced by chrysant farmer in North sumatera, indonesia is to control fungal disease caused by Fusarium oxysproum. The aims of this study were to know antagonistic bacterial isolates to reduce fungal disease caused by F. oxysporum and to know the isolates ability in producing plant growth hormon such as indole acetic acid and in solubilizing phosphate. Eight bacterial strains, collection of laboratory of Microbiology, Universitas sumatera Utara were tested against F. oxysporum in dual culture assay. two bacterial isolates, sP1, closely related to Bacillus amyloliquefaciens strain MPA 1034 and sP7R, closely related to Pseudomonas versuta strain l10.10 showed to reduce fusarium wilt in chrysant along with production of indole acetic acid and phosphate solubilizing properties in vitro. Application of the two antagonistic bacterial isolates resulted in considerably decrease of disease intensity caused by F. oxysporum. The results showed that utilization of bacterial isolate to promote chrysant performance is promising in the next future.

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Rhizobacteria induces resistance against Fusarium wilt of tomato by increasing the activity of defense enzymes

Cited by 28 publications

References 34 publications

Lactobacillus plantarum Exopolysaccharides Induce Resistance against Tomato Bacterial Spot

Lactobacillus plantarum Exopolysaccharides Induce Resistance against Tomato Bacterial Spot

Antagonistic rhizobacteria and jasmonic acid induce resistance against tomato bacterial spot

A Possibility of using Antagonistic Bacterial Isolates in Controlling Fusarium Wilt of Chrysanth (Chrysanthemum sp.)

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