Rhinovirus induction of fractalkine (CX3CL1) in airway and peripheral blood mononuclear cells in asthma

Upton, Nadine; Jackson, David J.; Nikonova, Alexandra; Hingley‐Wilson, Suzie; Khaitov, Musa; Rosario, Ajerico Del; Traub, Stephanie; Trujillo-Torralbo, Maria Belen; Habibi, Max; Elkin, Sarah; Kon, Onn Min; Edwards, Michael R.; Mallia, Patrick; Footitt, Joseph; Macintyre, Jonathan; Stanciu, Luminita A.; Johnston, Sebastian L.; Sykes, Annemarie

doi:10.1371/journal.pone.0183864

Cited by 9 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This data suggest that ML polarization is a dynamic process dependent on lung microenvironment and rhinovirus-induced asthma exacerbations in allergic patients is characterized by an amplified Th2 immune response. This in accordance with study of Upton et al where BAL cells from nonasthmatic controls and in M1-like MDM, but not BAL cells from mild asthmatics or M2-like MDM infected in vitro with RV were characterized by increased production of fractalkine [60] indirectly indicating decreased amounts of M1-like ML in allergic asthma patients.…”

Section: Discussionsupporting

confidence: 93%

M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations

Nikonova

Khaitov²,

Jackson

et al. 2020

EBioMedicine

Self Cite

View full text Add to dashboard Cite

Background: Macrophages (ML) can be M1/M2 polarized by Th1/2 signals, respectively. M2-like ML are thought to be important in asthma pathogenesis, and M1-like in anti-infective immunity, however their roles in virus-induced asthma exacerbations are unknown. Our objectives were (i) to assess polarised ML phenotype responses to rhinovirus (RV) infection in vitro and (ii) to assess ML phenotypes in healthy subjects and people with asthma before and during experimental RV infection in vivo. Methods: We investigated characteristics of polarized/unpolarized human monocyte-derived ML (MDM, from 3À6 independent donors) in vitro and evaluated frequencies of M1/M2-like bronchoalveolar lavage (BAL) ML in experimental RV-induced asthma exacerbation in 7 healthy controls and 17 (at baseline) and 18 (at day 4 post infection) people with asthma. Findings: We observed in vitro: M1-like but not M2-like or unpolarized MDM are potent producers of type I and III interferons in response to RV infection (P<0.0001), and M1-like are more resistant to RV infection (P<0.05); compared to M1-like, M2-like MDM constitutively produced higher levels of CCL22/MDC (P = 0.007) and CCL17/TARC (P<0.0001); RV-infected M1-like MDM were characterized as CD14 + CD80 + CD197 + (P = 0.002 vs M2-like, P<0.0001 vs unpolarized MDM). In vivo we found reduced percentages of M1-like CD14 + CD80 + CD197 + BAL ML in asthma during experimental RV16 infection compared to baseline (P = 0.024). Interpretation: Human M1-like BAL ML are likely important contributors to anti-viral immunity and their numbers are reduced in patients with allergic asthma during RV-induced asthma exacerbations. This mechanism may be one explanation why RV-triggered clinical and pathologic outcomes are more severe in allergic patients than in healthy subjects.

show abstract

Section: Discussionsupporting

confidence: 93%

M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations

Nikonova

Khaitov²,

Jackson

et al. 2020

EBioMedicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is produced in the intestinal tract following a trigger by proinflammatory signals and may be involved in directing intestinal epithelial cell-lymphocyte interactions, as well as the attraction of lymphocytes into the intestinal lamina propria. Recently it was reported that fractalkine may be involved in both immunopathological and antiviral immune responses to rhinovirus infection ( 23 ). Surface expression of CX3CR1, the receptor for fractalkine, has been demonstrated in NK cells, monocytes, CD8+ T cells, and, to a lesser extent, CD4+ T cells.…”

Section: Discussionmentioning

confidence: 99%

Fewer Community-Acquired Colds with Daily Consumption of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2. A Randomized, Placebo-Controlled Clinical Trial

Ahrén

Hillman

Nordström

et al. 2021

The Journal of Nutrition

View full text Add to dashboard Cite

Background Viral infections of the upper airways are the most common cause for absence from work or school, and there is evidence for probiotic efficacy in reducing the incidence and severity of these infections. Objectives We aimed to confirm the previously reported beneficial effects of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 against community-acquired common colds and identify a possible mechanism of action. Methods In a double-blind study, healthy adults (18–70 years of age) with at least 4 colds during the last 12 months before recruitment were randomly allocated to consume either probiotics (n = 448; total daily dose of 109 CFU with the 2 strains equally represented) or placebo (n = 450) once daily for 12 weeks. Recruitment took place from October to February during 2013–2016 (over 3 cold seasons). The probiotic impact on the severity of the colds (Wisconsin Upper Respiratory Symptom Survey−21) was the primary endpoint, whereas secondary endpoints included the incidence rate and duration of colds and an analysis of immune markers. Mann-Whitney U test and mixed model were used for the analysis of continuous variables and Fisher´s exact test was used for the analysis of categorical endpoints. Results Symptom severity was not reduced after intake of the probiotic, despite the positive trend seen in the first season. However, significantly fewer colds were experienced in the probiotic group (mean of 1.24 colds) as compared to the placebo group (mean of 1.36 colds; P = 0.044) for subjects reporting at least 1 cold, the incidence of recurring colds was 30% lower (20.8% vs. 29.8%, respectively; P = 0.055), and the use of analgesics was 18% lower (26.3% vs. 32%, respectively; P = 0.07). After 12 weeks, the change from baseline for IFN-γ differed between the groups (mean difference of −7.01; 95% CI, −14.9 to 0.93; P = 0.045). Conclusions Intake of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 can be protective against multiple colds in adults prone to getting colds. This trial was registered at clinicaltrials.gov as NCT02013934.

show abstract

“…However, in the convalescence phase (2 weeks later) the RV‐HBoV1 group was demonstrated by decreased expression of fractalkine, MCP‐3, and IL‐8 when compared with the RV group. Fractalkine is known for inducing chemotaxis and it has antiviral properties, 39 whereas MCP‐3 has been suggested as a profibrotic chemokine, 40 that is associated with activation and migration of monocytes and macrophages in the airways. 41 IL‐8, known for the robust chemotaxis of neutrophils in the airways, activates the inflammatory cells via the recruitment of innate and adaptive immunity cells.…”

Section: Discussionmentioning

confidence: 99%

Human bocavirus 1 coinfection is associated with decreased cytokine expression in the rhinovirus‐induced first wheezing episode in children

Hurme,

Sahla,

Rückert

et al. 2023

Clinical & Translational All

View full text Add to dashboard Cite

BackgroundRhinovirus (RV)‐induced first wheezing episodes in children are associated with a markedly increased risk of asthma. Previous studies have suggested that human bocavirus 1 (HBoV1) may modify RV‐induced immune responses in young children. We investigated cytokine profiles of sole RV‐ and dual RV‐HBoV1‐induced first wheezing episodes, and their association with severity and prognosis.MethodsFifty‐two children infected with only RV and nine children infected with dual RV‐HBoV1, aged 3–23 months, with severe first wheezing episodes were recruited. At acute illness and 2 weeks later, peripheral blood mononuclear cells were isolated, and stimulated with anti‐CD3/anti‐CD28 in vitro. Multiplex ELISA was used to quantitatively identify 56 different cytokines at both study points. Patients were prospectively followed for 4 years.ResultsThe mean age of the children was 14.3 months, and 30% were sensitized. During the acute illness, the adjusted analyses revealed a decrease in the expression of IL‐1b, MIP‐1b, Regulated upon activation, normal T cell expressed and presumably secreted (CCL5), TNF‐a, TARC, and ENA‐78 in the RV‐HBoV1 group compared with the RV group. In the convalescence phase, the RV‐HBoV1 group was characterized by decreased expression of Fractalkine, MCP‐3, and IL‐8 compared to the RV group. Furthermore, the hospitalization time was associated with the virus group and cytokine response (interaction p < 0.05), signifying that increased levels of epidermal growth factor and MIP‐1b were related with a shorter duration of hospitalization in the RV‐HBoV1 coinfection group but not in the RV group.ConclusionsDifferent cytokine response profiles were detected between the RV and the RV‐HBoV1 groups. Our results show the idea that RV‐induced immune responses may be suppressed by HBoV1.

show abstract

Rhinovirus induction of fractalkine (CX3CL1) in airway and peripheral blood mononuclear cells in asthma

Cited by 9 publications

References 34 publications

M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations

M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations

Fewer Community-Acquired Colds with Daily Consumption of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2. A Randomized, Placebo-Controlled Clinical Trial

Human bocavirus 1 coinfection is associated with decreased cytokine expression in the rhinovirus‐induced first wheezing episode in children

Contact Info

Product

Resources

About