Rheumatoid arthritis synovial fluid enhances T cell effector functions

Ridderstad, Anna; Abedi‐Valugerdi, Manuchehr; Ström, Håkan; Möller, Erna

doi:10.1016/0896-8411(92)90147-i

Cited by 2 publications

(2 citation statements)

References 49 publications

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“…Since CD4 + CD25 − and CD8 + T cells play a pivotal role in RA 19 and CD4 + CD25 − Helios + and CD8 + Helios + T cells have been reported as indicators of T cell proliferation and autoimmune reactivity 20 21 , we next analysed the proportions of these cells in thymus, spleen and ILNs of the CIA induced mice. Flow cytometer analysis showed that the total proportion of CD4 + CD8 − CD25 − and CD8 + CD4 − cells were lower in both the thymus and spleen of the Hpa-tg mice ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of heparanase enhances T lymphocyte activities and intensifies the inflammatory response in a model of murine rheumatoid arthritis

Digre

Singh

Åbrink

et al. 2017

Sci Rep

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Heparanase is an endo-glucuronidase that degrades heparan sulfate chains. The enzyme is expressed at a low level in normal organs; however, elevated expression of heparanase has been detected in several inflammatory conditions, e.g. in the synovial joints of rheumatoid arthritis (RA) patients. Herein, we have applied the model of collagen-induced arthritis (CIA) to transgenic mice overexpressing human heparanase (Hpa-tg) along with wildtype (WT) mice. About 50% of the induced animals developed clinical symptoms, i.e. swelling of joints, and there were no differences between the Hpa-tg and WT mice in the incidence of disease. However, Hpa-tg mice displayed an earlier response and developed more severe symptoms. Examination of cells from thymus, spleen and lymph nodes revealed increased innate and adaptive immune responses of the Hpa-tg mice, reflected by increased proportions of macrophages, antigen presenting cells and plasmacytoid dendritic cells as well as Helios-positive CD4+ and CD8+ T cells. Furthermore, splenic lymphocytes from Hpa-tg mice showed higher proliferation activity. Our results suggest that elevated expression of heparanase augmented both the innate and adaptive immune system and propagated inflammatory reactions in the murine RA model.

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Section: Resultsmentioning

confidence: 99%

Overexpression of heparanase enhances T lymphocyte activities and intensifies the inflammatory response in a model of murine rheumatoid arthritis

Digre

Singh

Åbrink

et al. 2017

Sci Rep

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“…Very recently, we have shown that RA-SF also acts on murine T cells and greatly augments the cytotoxic activity of T effector cells stimulated in a primary mouse mixed lymphocyte culture [5].…”

Section: Introductionmentioning

confidence: 98%

Partial Biochemical Characterization and Purification of IgG2b Inducing Factor as a New Cytokine from Synovial Fluid of Patients with Rheumatoid Arthritis

et al. 1993

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Rheumatoid arthritis synovial fluid (RA‐SF) contains a novel biological activity, which selectively induces IgG2b antibody production in lipopolysaccharide (LPS)‐activated mouse spleen cells in vitro and in vivo. Our previous studies have shown that this activity is not functionally identical to otherwell‐known cytokines and interleukins. In this study we demonstrate the partial purification and biochemical characterization of the IgG2b inducing activity in RA‐SF. Biochemical characterization revealed that the IgG2b inducing activity in RA‐SF has the following properties: it is a protein, sensitive to pH > 11 and < 4, which is precipitated by 50% of saturated ammonium sulphate and has a molecular weight of 50–70 kDa; it binds to Cibacron‐blue and heparin and its activity is not mediated by immunoglobulins or immune complexes, which are present in RA‐SF. Biochemical characteristics of the IgG2b inducing activity also differ from other cytokines and interleukins. The term IgG2b inducing factor is proposed for this novel activity.

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Rheumatoid arthritis synovial fluid enhances T cell effector functions

Cited by 2 publications

References 49 publications

Overexpression of heparanase enhances T lymphocyte activities and intensifies the inflammatory response in a model of murine rheumatoid arthritis

Overexpression of heparanase enhances T lymphocyte activities and intensifies the inflammatory response in a model of murine rheumatoid arthritis

Partial Biochemical Characterization and Purification of IgG2b Inducing Factor as a New Cytokine from Synovial Fluid of Patients with Rheumatoid Arthritis

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