The Rheotanytarsus muscicola species group is generally considered to be a taxonomically difficult group of non-biting midges (Diptera: Chironomidae). In this study, we review the R. muscicola species group based on the adult males from China using morphology and DNA barcodes. Rheotanytarsus ferringtoni Lin & Yao sp. n. is described and figured, and four species (Rheotanytarsus falcipedius Kyerematen, Andersen & Sæther, 2000; Rheotanytarsus fluminis Kawai & Sasa, 1985; Rheotanytarsus illiesi Siebert, 1979; and Rheotanytarsus photophilus Goetghebuer, 1921) new to China are redescribed, figured and discussed. An updated key to known adult males of the R. muscicola species group is presented. Our study shows concordance between morphological species concepts and DNA barcodes.