Aiming to improve the shear yield stress of magnetorheological fluid, magnetorheological fluids with different particle characteristics are prepared, and the influence rules of particle mass fraction, particle size, nanoparticles content and application temperature on shear yield stress are investigated. Experimental results indicate that shear yield stress increases approximate linearly with the enhancement of particle mass fraction. Particle size and the nanoparticles within 10% mass fraction can improve the shear yield stress effectively. When the application temperature is higher than 100 o C, the shear yield stress decreases rapidly because of thermal expansion and thermal magnetization effect.