Rheological studies of the fucose-rich exopolysaccharide FucoPol

Torres, Cristiana A.V.; Ferreira, Ana Rita; Freitas, Filomena; Reis, Maria A.M.; Coelhoso, Isabel M.; Sousa, Isabel; Alves, Vítor D.

doi:10.1016/j.ijbiomac.2015.05.029

Cited by 36 publications

(51 citation statements)

References 35 publications

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“…Thus, it can be speculated that higher concentrations of FucoPol increase the occurrence of polymer-polymer interaction, reducing the availability of binding sites capable of metal sequestration [21]. Furthermore, higher concentrations of FucoPol cause an increase in viscosity [41], which may affect the diffusion of the metals, thus restraining their interaction with less accessible binding sites. Similar results were reported in the literature for other EPS.…”

Section: Effect Of Fucopol Dosage On the Pb 2+ -Binding Ability Of Fucopolmentioning

confidence: 99%

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

2020

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Despite the efforts for minimizing the usage of heavy metals, anthropogenic activities still generate high amounts of wastewater containing these contaminants that cause significant health and environmental problems. Given the drawbacks of the conventional physical and chemical methods currently used, natural biosorbents (microbial cells or their products) arise as promising environmentally friendly alternatives. In this study, the binding efficiency of the polysaccharide secreted by Enterobacter A47, FucoPol, towards lead (Pb2+), cobalt (Co2+), copper (Cu2+) and zinc (Zn2+) cations was demonstrated. FucoPol revealed a higher performance for the biosorption of Pb2+, with a maximum overall metal removal of 93.9 ± 5.3% and a specific metal uptake of 41.1 ± 2.3 mg/gEPS, from a Pb2+ solution with an initial concentration of 10 mg/L, by a 5 g/L FucoPol solution. The overall metal removal decreased considerably (≤31.3 ± 1.6%) for higher Pb2+ concentrations (48 and 100 mg/L) probably due to the saturation of FucoPol’s binding sites. Pb2+ removal was also less efficient (66.0 ± 8.2%) when a higher FucoPol concentration (10 g/L) was tested. Pb2+ removal efficiency of FucoPol was maximized at pH 4.3, however, it was affected by lower pH values (2.5–3.3). Moreover, the FucoPol’s sorption performance was unaffected (overall metal removal: 91.6–93.9%) in the temperature range of 5–40 °C. These findings demonstrate FucoPol’s great potential for utilization as a biodegradable and safe biosorbent for treating waters and wastewaters contaminated with Pb2+.

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Section: Effect Of Fucopol Dosage On the Pb 2+ -Binding Ability Of Fucopolmentioning

confidence: 99%

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

2020

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“…The oxidized Fe 3+ can then react again with H 2 O 2 to form a second toxic molecule, the perhydroxyl radical HOO • , which is highly damaging to cellular structures by promoting the continuous progression of current oxidative cascades and the development of new ones. FucoPol is a polyanionic polysaccharide because it contains approximately 22 wt% of negatively charged succinyl, pyruvyl, and glucuronic acid groups in its structure [ 22 ]. Its negatively charged residues can then participate in redox reactions and scavenge oxidative radicals at physiological pH, thus maintaining cellular homeostatic balance.…”

Section: Discussionmentioning

confidence: 99%

“…First, it acts as a crystallization inhibitor, successfully cryopreserving several animal cell lines [19,20]. Second, it has a rheological shear-thinning behavior [22] and emulsifying properties [18,23], which are highly desirable in the food industry. Recently, it has been shown to provide sunscreen-like photoprotective action to epithelial cells acutely exposed to UV radiation [21], a mechanism by which ROS cascades are also triggered.…”

Section: Introductionmentioning

confidence: 99%

“…FucoPol has a fucose, galactose, glucose, and glucuronic acid hexamer motif (2.0:1.9:0.9:0.5 M ratio), a main chain composed of a →4)-α- l -Fuc p -(1→4)-α- l -Fuc p -(1→3)-β- d -Glc p (1→ trimer repeating unit, and a trimer branch α- d -4,6-pyruvyl-Gal p -(1→4)-β- d -GlcA p -(1→3)-α- d -Gal p (1→ in the C-3 of the first fucose [ 19 ] ( Figure S1 ). FucoPol also contains 13–14 wt% pyruvyl, 3–5 wt% acetyl, and 2–3 wt% succinyl in its acyl composition [ 22 ]. The presence of glucuronic acid as well as the acyl substituents pyruvyl and succinyl all confer a polyanionic character to the polymer [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

et al. 2021

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Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by exogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its structure. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo- and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.

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“…FucoPol has demonstrated flocculating and emulsion stabilizing capacity, comparable to commercial polymers [ 110 ]. FucoPol has also shown to have a good thickening capacity in various aqueous formulations (with a wide range of pH and ionic strength) [ 111 ]. These functional properties make this polymer a good alternative in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries.…”

Section: Polysaccharides In Food Packagingmentioning

confidence: 99%

Polysaccharide-Based Membranes in Food Packaging Applications

2016

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Plastic packaging is essential nowadays. However, the huge environmental problem caused by landfill disposal of non-biodegradable polymers in the end of life has to be minimized and preferentially eliminated. The solution may rely on the use of biopolymers, in particular polysaccharides. These macromolecules with film-forming properties are able to produce attracting biodegradable materials, possibly applicable in food packaging. Despite all advantages of using polysaccharides obtained from different sources, some drawbacks, mostly related to their low resistance to water, mechanical performance and price, have hindered their wider use and commercialization. Nevertheless, with increasing attention and research on this field, it has been possible to trace some strategies to overcome the problems and recognize solutions. This review summarizes some of the most used polysaccharides in food packaging applications.

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Rheological studies of the fucose-rich exopolysaccharide FucoPol

Cited by 36 publications

References 35 publications

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol

Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

Polysaccharide-Based Membranes in Food Packaging Applications

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