Rheological properties of fresh and cryopreserved human arteries tested in vitro

Blondel, Walter; Lehalle, Bertrand; Maurice, G.; Wang, Xiong; Stoltz, Jean‐François

doi:10.1007/s003970000110

Cited by 24 publications

(28 citation statements)

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“…fissuring, rupture, aneurysm) (Lehalle et al 1997). Whether the cryopreserved procedures modify viscoelastic and inertial wall properties and geometrical arterial properties remains controversial (Blondel et al 2000;Buja´n et al 2001).…”

Section: Discussionmentioning

confidence: 95%

Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis

Bia

Pessana

Armentano

et al. 2006

Cell Tissue Banking

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The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The aim of this work was to assess the cryopreservation's effects on human arteries mechanical properties. Common carotid artery (CCA) segments harvested from donors were divided into two groups: Fresh (n = 18), tested for 24-48 h after harvesting, and Cryopreserved (n = 18) for an average time of 30 days in gas-nitrogen phase, and finally defrosted. Each segment was tested in a circulation mock, and its pressure and diameter were registered at similar pump frequency, pulse and mean pressure levels, including those of normotensive and hypertensive conditions. A compliance transfer function (diameter/pressure) derived from a mathematical adaptive modelling was designed for the on line assessment of the arterial wall dynamics and its frequency response. Assessment of arterial wall dynamics was made by measuring its viscous (eta), inertial (M) and elastic (E) properties, and creep and stress relaxation time constant (tauC and tauSR, respectively). The frequency response characterization allowed to evaluate the arterial wall filter or buffer function. Results showed that non-significant differences exist between wall dynamics and buffer function of fresh and cryopreserved segments of human CCA. In conclusion, our cryopreservation method maintains arterial wall functional properties, close to their fresh values.

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Section: Discussionmentioning

confidence: 95%

Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis

Bia

Pessana

Armentano

et al. 2006

Cell Tissue Banking

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“…However, Blondel et al did see a difference in cryopreserved SFA subjected to inflation tests. 3 The main differences observed were in the measured strains and stresses, but not in the calculated elastic moduli or compliance values. These results are difficult to compare directly to our results since the type of test was different.…”

Section: Comparison To Other Workmentioning

confidence: 90%

“…The arteries are assumed to be circular and cylindrical thin tubes. 3 The linear regime in the stress-strain curve for fresh samples started at higher strains while it was considerably lower for frozen samples. After thawing the frozen samples and culturing at 37 • C for 24 h, a shift towards the fresh behavior was observed, though not completely (Fig.…”

Section: Stress-strain Characteristics Of Frozen Arteriesmentioning

confidence: 98%

Effects of Freezing and Cryopreservation on the Mechanical Properties of Arteries

et al. 2006

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Cryoplasty, a freezing therapy, is being used for the treatment of restenosis in peripheral arteries. In addition, cryo-preserved arteries are increasingly used in vascular grafts. While studies are being performed to establish the efficacy of such treatments, very little is known about the postcryosurgical or postcryo-preservation changes in mechanical properties of the arteries. Few studies have examined the effect of freezing in the absence of cryoprotective agents (CPAs), and the several studies done in the presence of CPAs have given mixed results. To examine this issue further, we froze pig femoral arteries in a controlled rate freezer, using an aluminum probe, both in the presence at (-80 degrees C to 1 degrees C/min) and absence (at -20 degrees C for 2 or 5 mins) of CPA and Fetal bovine serum (FBS). Following freezing, artery samples were subjected to uniaxial tensile testing. The weights of the tissue were measured before and after freezing. Our results suggest that freezing does have an effect on stress-strain properties, particularly in the low stress region corresponding to physiological conditions. The mechanisms of this change in mechanical properties may include the loss of smooth muscle cell viability, damage to extra cellular matrix (ECM), bulk redistribution of water, or changes in alignment caused by ice crystal growth. In the case of samples frozen in the absence of CPA or FBS, the results indicated a drastic reduction in weight of the tissue suggesting the importance of bulk water redistribution as one underlying mechanism. To further examine potential mechanisms, we subjected cryopreserved vessels to the same uniaxial tests. The extent of changes in mechanical properties and bulk water redistribution was greatly attenuated; reinforcing that water movement might play a role in the changes observed with freezing.

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“…[38] Despite numerous efforts that have been made to improve the vascular allograft cryopreservation, poor results were observed especially due to changes in the mechanical properties of the tissue. [7,8,12] Moreover, similar mechanical properties of the graft and the recipient vascular conduit appeared to be important to prevent intimae hyperplasia, which corresponds to an increase of the cell number and of the size of the tissue, occlusion, and dilatation of the blood vessels. [39,40] In fact, the cryopreservation procedure as well as the enzymatic treatment may also alter the collagen/elastin network and modify the mechanical properties of the arteries.…”

Section: Full Papermentioning

confidence: 99%

Re‐endothelialization of Human Umbilical Arteries Treated with Polyelectrolyte Multilayers: A Tool for Damaged Vessel Replacement

Kerdjoudj

Boura

Moby

et al. 2007

Adv Funct Materials

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The use of cryopreserved arteries for vascular tissue engineering provides a promising way for vessel replacement. Unfortunately cryopreservation induces structural changes that strongly modify the mechanical properties and alter the thrombogenicity of the vessel after implantation. We present here a new procedure to treat the inner coating of cryopreserved arteries with poly(sodium‐4‐styrene sulfonate)/poly(allylamine hydrochloride) polyelectrolyte multilayers. We show that this treatment improves the mechanical properties of the cryopreserved vessel. It also allows the adhesion and spreading of endothelial cells so that the internal structure of the vessel closely resembles that of fresh arteries. Finally, we verify by PECAM‐1 and von‐Willebrand‐factor (vWF) expression that this treatment preserves the phenotype of the endothelial cells. This study should open new routes towards the development of future, new biocompatible tissue substitutes allowing long‐term functionality after implantation.

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Rheological properties of fresh and cryopreserved human arteries tested in vitro

Cited by 24 publications

References 0 publications

Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis

Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis

Effects of Freezing and Cryopreservation on the Mechanical Properties of Arteries

Re‐endothelialization of Human Umbilical Arteries Treated with Polyelectrolyte Multilayers: A Tool for Damaged Vessel Replacement

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