Rheological investigation on hyaluronan–fibrinogen interaction

Rinaudo, Marguerite

doi:10.1016/j.ijbiomac.2008.08.009

Cited by 18 publications

(13 citation statements)

References 18 publications

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“…The main factor influencing the SF rheologic behavior seems to be the level of dilution as a consequence of joint effusion [40,55]. One other important factor may be the interaction between proteins and HA, leading to complex formations whose structure may be influenced by the composition of the proteins [46]. Our data suggest the rheologic properties of SF are unchanged over 6 weeks.…”

Section: Discussionmentioning

confidence: 70%

“…The viscoelastic behavior involves entanglements in HA and/or protein-HA associations based mainly on electrostatic interactions [46]. As HA is an anionic polyelectrolyte able to interact with positive charges on proteins, the viscoelasticity of SF may be related to the formation of a complex of HA and proteins forming a physical threedimensional network [17,29,57,58].…”

Section: Introductionmentioning

confidence: 99%

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Rheologic Behavior of Osteoarthritic Synovial Fluid after Addition of Hyaluronic Acid: A Pilot Study

Mathieu

Conrozier

Vignon

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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Viscosupplementation is a symptomatic treatment of osteoarthritis (OA) intended to restore rheologic homeostasis of the synovial fluid by injecting hyaluronic acid intraarticularly. Despite the long history of this therapy, little is known about its mechanisms of action and differences between commercial preparations. We investigated the rheologic behavior of OA synovial fluid with time, when stored at 4°C, before and after the addition of two hyaluronic acid commercial preparations (linear and cross-linked). Thirteen OA synovial fluids were stored at 4°C and assayed using steric exclusion chromatography, which allows hyaluronic acid to be separated from the remaining pool of proteins and its molecular weight and concentration to be determined without any pretreatment and calibration. The synovial fluid rheology also was studied in vitro, before and after addition of two viscosupplements, over 6 weeks. The non-Newtonian behavior of synovial fluid throughout followup appears to be the result of loose interactions between proteins and hyaluronic acid. When mixed with the linear hyaluronic acid, synovial fluid becomes less non-Newtonian whereas the non-Newtonian behavior was reinforced when mixed with the cross-linked hyaluronic acid. The rheology was nearly unchanged for all synovial fluids over 6 weeks. Our preliminary trial shows it is possible to study synovial fluid, stored at 4°C, over a long time and suggests the enzymatic degradation of hyaluronic acid is negligible under these experimental conditions.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Rheologic Behavior of Osteoarthritic Synovial Fluid after Addition of Hyaluronic Acid: A Pilot Study

Mathieu

Conrozier

Vignon

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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“…Nevertheless, as was previously shown, HA-protein complex (even if only small amount of polymers is engaged) exists in SF which causes a particular non-Newtonian behaviour [3,25]. We have recently studied the influence of fibrinogen as a model of protein on the rheology of HA, and demonstrated the physical interaction at neutral pH which should be based on electrostatic interactions [1]. The viscometric characterization of such systems does not allow the direct determination of the role of HA molecular weight and/or concentration on the SF behaviour if the proteins are not completely separated from HA as previously claimed by Ogston and Sherman [7].…”

Section: Resultsmentioning

confidence: 80%

“…Synovial fluid (SF) is a viscoelastic system with a characteristic non-Newtonian behaviour, consisting of hyaluronan (HA) and proteins, at least partly involved in a loose physical 3D-network based on secondary interactions of electrostatic nature [1]. The existence of such a complex has been discussed at length in the literature [2][3][4][5][6][7][8] and it is still under debate: on one hand, non covalent protein-HA interactions seems to be admitted by many authors at neutral pH [3][4][5][6][7][8]; on the other hand, HA and proteins are claimed to be separated in the SF fluid [9] and other authors consider that the rheological behaviour depends essentially on the HA contribution [10,11].…”

Section: Introductionmentioning

confidence: 99%

Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids

Rinaudo

Rozand²,

Mathieu

et al. 2009

Polymers

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Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the steady state viscosity is always non-Newtonian and not directly related to SF composition. Pre-treatment of SF (storage in cold, filtration, centrifugation) allows us to conclude that, in order to store SF, it is best to freeze it, even if in some cases, viscosity is modified but not the composition. All the data obtained (including protease pre-treatment) allow us to conclude that a small fraction of HA-protein complex forms a loose 3D-network and controls the rheology.

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“…After freeze‐drying, the fiber structure was slightly disrupted, and the hydrophilic parts of HA could have been exposed to the surface, which enable the coupling with proteins. The interaction of FNG with HA through fibrin‐ and heparin‐binding domain at the N‐terminal was previously described as well as binding of FNG to HA through electrostatic interactions . The interaction of HA with COL IV has not been studied yet, however, the mixture of both compounds was used to cover the titanium implants and their presence enable adhesion and contraction of smooth muscle cells .…”

Section: Resultsmentioning

confidence: 99%

Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

Sogorkova

Zapotocky

Čepa

et al. 2018

J Biomedical Materials Res

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Polysaccharides meet several criteria for a suitable biomaterial for tissue engineering, which include biocompatibility and ability to support the delivery and growth of cells. Nevertheless, most of these polysaccharides, for example dextran, alginate, and glycosaminoglycans, are highly soluble in aqueous solutions. Hyaluronic acid hydrophobized by palmitic acid and processed to the form of wet-spun fibers and the warp-knitted textile scaffold is water non-soluble, but biodegradable material, which could be used for the tissue engineering purpose. However, its surface quality does not allow cell attachment. To enhance the biocompatibility the surface of palmitoyl-hyaluronan was roughened by freeze drying and treated by different cell adhesive proteins (fibronectin, fibrinogen, laminin, methacrylated gelatin and collagen IV). Except for collagen IV, these proteins covered the fibers uniformly for an extended period of time and supported the adhesion and cultivation of dermal fibroblasts and mesenchymal stem cells. Interestingly, adipose stem cells cultivated on the fibronectin-modified scaffold secreted increasing amount of HGF, SDF-1, and VEGF, three key growth factors involved in cardiac regeneration. These results suggested that palmitoyl-hyaluronan scaffold may be a promising material for various applications in tissue regeneration, including cardiac tissue repair. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1488-1499, 2018.

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Rheological investigation on hyaluronan–fibrinogen interaction

Cited by 18 publications

References 18 publications

Rheologic Behavior of Osteoarthritic Synovial Fluid after Addition of Hyaluronic Acid: A Pilot Study

Rheologic Behavior of Osteoarthritic Synovial Fluid after Addition of Hyaluronic Acid: A Pilot Study

Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids

Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

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