Rheological characterization of poly-dimethyl siloxane formulations with tunable viscoelastic properties

Petet, Thomas J.; Deal, Halston; Zhao, Hanhsen S.; He, Amanda Y.; Tang, Christina; Lemmon, Christopher A.

doi:10.1039/d1ra03548g

Cited by 9 publications

(9 citation statements)

References 41 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At low strains, Sylgard 527 has previously been shown to be linear elastic 55 . Additionally, the rheological testing (1 rad/s = 0.1592 Hz) and the spontaneously beating μHM (≤1 Hz) both occurred at low frequencies, over which there is little frequency dependence in the shear modulus of Sylgard 527 56 . As such, the dynamic and static modulus should be similar.…”

Section: Discussionmentioning

confidence: 94%

“…55 Additionally, the rheological testing (1 rad/ s = 0.1592 Hz) and the spontaneously beating μHM (≤1 Hz) both occurred at low frequencies, over which there is little frequency dependence in the shear modulus of Sylgard 527. 56 As such, the dynamic and static modulus should be similar. This expectation is consistent with our results, which suggested no significant difference between the shear modulus measured using this platform and shear storage modulus measured using the rheometer.…”

Section: Reversibility Of Changes In μHm Physiology Induced By Acute ...mentioning

confidence: 99%

See 1 more Smart Citation

Dynamic control of contractile resistance to iPSC‐derived micro‐heart muscle arrays

Schuftan,

Kooh,

Guo

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

Many types of cardiovascular disease are linked to the mechanical forces placed on the heart. However, our understanding of how mechanical forces exactly affect the cellular biology of the heart remains incomplete. In vitro models based on cardiomyocytes derived from human induced pluripotent stem cells (iPSC‐CM) enable researchers to develop medium to high‐throughput systems to study cardiac mechanobiology at the cellular level. Previous models have been developed to enable the study of mechanical forces, such as cardiac afterload. However, most of these models require exogenous extracellular matrix (ECM) to form cardiac tissues. Recently, a system was developed to simulate changes in afterload by grafting ECM‐free micro‐heart muscle arrays to elastomeric substrates of discrete stiffnesses. In the present study, we extended this system by combining the elastomer‐grafted tissue arrays with a magnetorheological elastomeric substrate. This system allows iPSC‐CM based micro‐heart muscle arrays to experience dynamic changes in contractile resistance to mimic dynamically altered afterload. Acute changes in substrate stiffness led to acute changes in the calcium dynamics and contractile forces, illustrating the system's ability to dynamically elicit changes in tissue mechanics by dynamically changing contractile resistance.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Reversibility Of Changes In μHm Physiology Induced By Acute ...mentioning

confidence: 99%

Dynamic control of contractile resistance to iPSC‐derived micro‐heart muscle arrays

Schuftan,

Kooh,

Guo

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Although mechanobiology research has focused on the elastic component of soft tissue mechanical properties, soft tissues in the human body have viscoelastic behaviour. 61 Viscoelasticity is the property of materials that exhibit both viscous and elastic behaviour. 62 The elastic or storage modulus is related to the specimen's stiffness, and the viscous or loss modulus correlates with the specimen's ability to dissipate mechanical energy.…”

Section: Resultsmentioning

confidence: 99%

Developing tuneable viscoelastic silicone gel-based inks for precise 3D printing of clinical phantoms

Nieva-Esteve,

Agulló,

Grande-Molina

et al. 2024

Mater. Adv.

View full text Add to dashboard Cite

show abstract

“…Fromell et al found that more flexible polymer chains seemed to increase protein packing and encourage adsorption of proteins in their native conformation, while stiffer chains encouraged a change in conformation. 51 Here, we have mixed Sylgard 527 which is composed of chains with more repeat units than Sylgard 184, 52 explaining the decrease in elastic modulus seen with increasing amounts of Sylgard 527. It is important to consider when analyzing these results that this method of detection using the EIA determines the exposure of proteins for antibody binding, and functional sites could be hidden on certain substrates due to polymer chain directed changes in conformation when performing adsorption in a competitive plasma protein environment, more representative of what would be experienced physiologically.…”

Section: Fetuin-a Adsorption From Competitive Protein Environment (Pl...mentioning

confidence: 88%

Fetuin‐A adsorption to tunable polydimethylsiloxane and subsequent macrophage response

Miller

Sask

2023

J Biomedical Materials Res

View full text Add to dashboard Cite

Surface modifications can be applied to biomaterials to alter the various surface properties that influence protein‐material interactions and the cellular response. The plasma protein fetuin‐A has been found to adsorb to many biomaterials but details of its interactions with polydimethylsiloxane (PDMS) and roles in regulating the immune response are not clear. Here, PDMS modifications are achieved by altering the ratio of PDMS formulations to control elastic modulus, and by coating PDMS with polydopamine (PDA) to attach fetuin‐A. Surface characterization confirmed that altering the PDMS formulation changed the elastic modulus without affecting surface wetting properties. Surface roughness was measured using atomic force microscopy and surface chemistry was determined using X‐ray photoelectron spectroscopy, with only minor changes detected on the softest samples. PDA deposition on PDMS was confirmed and contact angle measurements demonstrated an increase in hydrophilicity. Fetuin‐A adsorption was influenced by the PDMS formulations, adsorption changed in a competitive plasma environment, and PDA was able to immobilize the greatest amount of fetuin‐A. The inflammatory effects of fetuin‐A were investigated, and data suggests that the elastic modulus influences cytokine secretion from macrophages at certain timepoints, a result likely due to varied protein amounts and orientations/conformations in response to material stiffness. The addition of a PDA layer demonstrated the potentially cytokine mitigating effect upon fetuin‐A immobilization when compared to unmodified PDMS samples. The results provide new insight into the interactions of fetuin‐A with PDMS and PDA, and the potential immune regulatory properties of fetuin‐A modified materials.

show abstract

Rheological characterization of poly-dimethyl siloxane formulations with tunable viscoelastic properties

Abstract: We have generated novel formulations of polydimethyl siloxane with varying viscoelastic properties that can be used to study cellular response. We present equations that can be used to predict the storage and loss moduli of these polymers.

Cited by 9 publications

References 41 publications

Dynamic control of contractile resistance to iPSC‐derived micro‐heart muscle arrays

Dynamic control of contractile resistance to iPSC‐derived micro‐heart muscle arrays

Developing tuneable viscoelastic silicone gel-based inks for precise 3D printing of clinical phantoms

Fetuin‐A adsorption to tunable polydimethylsiloxane and subsequent macrophage response

Contact Info

Product

Resources

About