Rheological and Microbiological Study on Bio-Composites with Marine Chitosan Polymers from Black Sea Stone Crabs used in Medical Therapy of Tissue Regeneration

Sîrbu, Rodica; Mustafa, Alef; Tomescu, Aneta; Stanciu, Gabriela; Cadâr, Emin

doi:10.37358/mp.19.1.5141

Cited by 6 publications

(8 citation statements)

References 18 publications

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“…The antioxidant capacity was evaluated using DPPH Radical Scavenging test. Gallic acid (GA) was used as standard to plot calibration curves and the results were expressed as equivalents (mg GAE) [14,16,[27][28][29][30][31][32]. In 25 mL calibrated flasks different volume of gallic acid solutions were added, then 5 mL DPPH 0.063% (1.268 mM) in methanol, filled up to the mark with methanol and let in the dark, to the room temperature for 45 minutes before the absorbance registration at 530 nm versus methanol.…”

Section: Dpph Radical Scavenging Testmentioning

confidence: 99%

Quantitative Analysis of Polyphenols and Biological Activity of Sage Macerates

Stanciu¹,

Lupsor²,

Aonofriesei³

et al. 2019

Rev.Chim.

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The total phenols concentration in two sage macerates has been estimated by Folin-Ciocâlteau method, identified and quantified using HPLC-DAD method in order to assess the biological activity. The results for total phenols values of Folin Ciocalteau method indicate that Salvia officinalis L macerate S2, presents a higher amount of phenolic compounds than macerate S1. By HPLC-DAD method, six individual phenolic compounds were identified in sage macerates among which where cinnamic acid was found in highest concentration (652.478 mg/100g d.w. in S2 and 473.381 mg/100g d.w. in S1). The antioxidant activity of sage macerates was evaluated using DPPH Radical Scavenging test. Sage macerates exhibited high antioxidant activity, between 439.5 mg GAE /mL and 400 mg GAE /mL. Antibacterial activity of sage macerates was evaluated against 20 Gram positive and Gram negative bacterial strains isolated from clinical specimens. Both macerates showed significant but variable antibacterial activity with inhibition zones ranging from 4.97 mm (S2) to 7.28.mm (S1). The effect was stronger on Gram positive (Enterococcus, Staphyococcus) than Gram negative bacteria (Escherichia sp, Proteus sp, Klebsiella sp). Eleven metals concentrations were determined by AAS method in sage leaves; it has been found that Cd, Ni and Pb concentrations are below the detection limits.

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Section: Dpph Radical Scavenging Testmentioning

confidence: 99%

Quantitative Analysis of Polyphenols and Biological Activity of Sage Macerates

Stanciu¹,

Lupsor²,

Aonofriesei³

et al. 2019

Rev.Chim.

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“…Due to their natural origin, both chitin and chitosan are defined as a family of polymers which present a high variability in their chemical and biological properties such as biocompatibility, biodegradability, mucoadhesion, anticholesterolemic, antitumoral, hemostatic and antimicrobial effect (Sirbu R., 2019).…”

Section: Fig2 Structure Of Chitosanmentioning

confidence: 99%

“…This variability is related not only to the origin of the samples but also to their method of preparation. So, a complete characterization of the samples is necessary (Sirbu R., 2019).…”

Section: Introductionmentioning

confidence: 99%

Studies on Chitosan, Chitin and Chitooligosaccharides and Their Biomedical Properties

Ibram

2021

European Journal of Natural Sciences and Medicine

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Research in healthcare involves the use of natural resources in the manufacture of pharmaceutical products. Chito-oligosaccharides (CHOS) are described as homo- or heterooligomers of N-acetylglucosamine and D-glucosamine. As a starting material for CHOS production chitin or chitosan can be used. When it comes to the medical field, there are indications that CHOS may be developed and used as drugs against asthma, antibacterial agents, ingredients in wound-dressings and vectors in gene-therapy. The methods of production can rely on enzymatic conversions, chemical methods or combinations of these methods. There is ample literature concerning the biological effects of chitosans, but relatively little is known about the effects of CHOS, and the mechanisms behind observed bioactivities are generally poorly understood. This review is focused on the characterization of chitin and chitosan by presenting the biological properties, and on the enzymatic production of CHOS, and on further separation and purification methods for producing well-defined mixtures and also provides an overview of some of the most promising applications of CHOS.

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“…Alexander Fleming highlighted the importance of chemistry research in designing and modifying molecules to develop new drugs or to enhance the properties of the existing ones [9,[49][50][51]. After decades, in the context of antimicrobial resistance this is more necessary than ever [9].…”

Section: Figure 6 Distribution Of Antimicrobial Resistance Accordingmentioning

confidence: 99%

The Need to Develop New Antimicrobial Molecules, as Revealed by in vitro Assessment of Drug Resistance in Staphylococcal Skin Infections Treated with Autologous Bacterial Vaccine

Preda¹,

Serbanescu²,

Mihai³

et al. 2020

Rev. Chim.

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Staphylococcus spp. is a facultative pathogen, which can be found in the commensal microbiota of humans, most often in moist skinfolds and mucous membranes. This microorganism has the ability to cause various infections, in almost every organ of the body, with an increased frequency in the skin and soft tissues, being involved in pathologies like acne, folliculitis, furunculosis, hidradenitis suppurativa, cellulitis, abscesses, but also in secondary infections in diseases with an altered cutaneous barrier. The prolonged evolution of these diseases and severe outcome can be influenced by various factors, most importantly being the antimicrobial resistance. We have evaluated the antimicrobial susceptibility profiles, according to the Comite de l` Antibiogramme de la Societe Francaise de Microbiologie recommendations, for strains of Staphylococcus spp. isolated from acne or different types of skin and soft tissue infections in patients recommended to receive autologous bacterial vaccine. Most frequent identified species was Staphylococcus epidermidis, followed by Staphylococcus aureus. The antimicrobial resistance was higher for antibiotics usually used in the treatment of skin and soft tissue infections, with interesting differences of the resistance profile for the strains isolated from patients before receiving autologous bacterial vaccine compared with the ones from individuals already treated. Another important finding was represented by the differences in the resistance profile according to the age group of the patients. The results of this study underline the importance of antimicrobial resistance surveillance in finding new molecules and alternative therapies, the necessity of a personalized approach in medical acts and of a continuous connection between clinic and laboratory research.

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Rheological and Microbiological Study on Bio-Composites with Marine Chitosan Polymers from Black Sea Stone Crabs used in Medical Therapy of Tissue Regeneration

Cited by 6 publications

References 18 publications

Quantitative Analysis of Polyphenols and Biological Activity of Sage Macerates

Quantitative Analysis of Polyphenols and Biological Activity of Sage Macerates

Studies on Chitosan, Chitin and Chitooligosaccharides and Their Biomedical Properties

The Need to Develop New Antimicrobial Molecules, as Revealed by in vitro Assessment of Drug Resistance in Staphylococcal Skin Infections Treated with Autologous Bacterial Vaccine

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