Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs

Zhuang, Songlin; Zhong, Jia; Bian, Yifei; Fan, Yu; Chen, Qiyan; Liu, Ping; Liu, Zhongjie

doi:10.1016/j.lfs.2018.11.048

Cited by 71 publications

(67 citation statements)

References 45 publications

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“…The destructed villus morphology and inflammatory reaction were induced by LPS injection, which decreased the growth performance of chicks. These phenotypes indicated that intestinal injury model had been successfully established, which is consistent with the previous researches ( Wang et al., 2019b , Zhuang et al., 2019 ). In line with our expectations, genistein treatment alleviated LPS-induced intestinal damage and improved growth performance of chicks.…”

Section: Discussionsupporting

confidence: 90%

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Dai

Song

et al. 2020

Poultry Science

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Genistein is abundant in the corn-soybean meal feed. Little information is available about the effect of dietary genistein on the intestinal transcriptome of chicks, especially when suffering from intestinal injury. In this study, 180 one-day-old male ROSS 308 broiler chickens were randomly allocated to 3 groups, with 4 replicates (cages) of 15 birds each. The treatments were as follows: chicks received a basal diet (CON), a basal diet and underwent lipopolysaccharide-challenge (LPS), or a basal diet supplemented with 40 mg/kg genistein and underwent LPS-challenge (GEN). LPS injection induced intestinal injury and inflammatory reactions in the chicks. Transcriptomic analysis identified 7,131 differently expressed genes (3,281 upregulated and 3,851 downregulated) in the GEN group compared with the LPS group ( P adjusted value < 0.05, |fold change| > 1.5), which revealed that dietary genistein exposure altered the gene expression profile and signaling pathways in the ileum of LPS-treated chicks. Furthermore, dietary genistein improved intestinal morphology, mucosal immune function, tight junction, antioxidant activity, apoptotic process, and growth performance, which were adversely damaged by LPS injection. Therefore, adding genistein into the diet of chicks can alter RNA expression profile and ameliorate intestinal injury in LPS-challenged chicks, thereby improving the growth performance of chicks with intestinal injury.

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Section: Discussionsupporting

confidence: 90%

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Dai

Song

et al. 2020

Poultry Science

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“…Therefore, oxidative stress inhibition may be an effective strategy to repair intestinal barrier damage [38]. The MDA, a lipid peroxidation product, is a marker of oxidative stress and is considered as an indicator to reflect the degree of cell damage and lipid peroxidation [3,38]. In our study, Mean values with unlike superscript letters were significantly different (P < 0.05)…”

Section: Discussionmentioning

confidence: 58%

“…As we know, weaning stress of piglet leads to excessive production of reactive oxygen species (ROS), which breaks the balance between oxidation system and antioxidant system, forms oxidative stress and leads to tissue damage, including intestinal barrier injury. Therefore, oxidative stress inhibition may be an effective strategy to repair intestinal barrier damage [38]. The MDA, a lipid peroxidation product, is a marker of oxidative stress and is considered as an indicator to reflect the degree of cell damage and lipid peroxidation [3,38].…”

Section: Discussionmentioning

confidence: 99%

Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets

Song

et al. 2020

J Animal Sci Biotechnol

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Background: Tannic acid (TA) is potential to reduce diarrhea in weaning pigs, but knowledge about the influence of TA on intestinal barrier integrity and function is still scarce. This experiment was conducted to investigate the effects of dietary TA supplementation on growth performance, diarrhea rate, intestinal barrier integrity and function of weaned pigs. Methods: A total of 108 crossbred (Duroc × Landrace × Yorkshire) piglets, with an initial average body weight of 6.60 ± 0.27 kg, were allotted to 3 groups (6 pigs/pen and 6 replicates/group) in a randomized complete block design according to their gender and body weight. Piglets were fed the basal diet with 0 (control, CON), 0.2% and 1.0% TA, respectively. The trial lasted for 28 d. Results: Compared with the CON group, dietary 0.2% and 1.0% TA supplementation didn't affect ADFI, ADG and F: G (P > 0.05), but reduced diarrhea rate, diarrhea index and diarrhea score of piglets (P < 0.05), reduced diamine oxidase (DAO) activity and D-lactic acid concentration in serum (P < 0.01). The higher occludin expression and localization were observed in the duodenum, jejunum and ileum after supplementation with 0.2% or 1.0% TA (P < 0.05). Adding 0.2% TA to diet significantly decreased crypt depth, increased villus height/crypt depth ratio in the duodenum (P < 0.05), and dietary 1.0% TA tended to decrease crypt depth (P < 0.10) and significantly decreased villus height (P < 0.05) of the ileum. Moreover, lower malondialdehyde content in the ileum was detected in the pigs fed 1.0% TA (P < 0.05). In the duodenum, both 0.2% and 1.0% TA groups had higher occludin (OCLN) mRNA and 0.2% TA group had higher zonula occludens-2 (ZO-2) level (P < 0.05). Meanwhile, dietary 1.0% TA supplementation tended to up-regulate OCLN mRNA levels in the jejunum (P < 0.10) and 0.2% TA supplementation tended to up-regulate zonula occludens-1 (ZO-1) mRNA levels in the ileum (P < 0.10). Conclusion: In conclusion, dietary supplementation of 0.2% or 1.0% TA could effectively alleviate postweaning diarrhea without altering growth performance in weaned piglets, which might be achieved by improving intestinal barrier integrity and function.

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“…The recovery of motor function is often determined by nerve repair after SCI . Rhubarb has effects against cerebral ischemia injury, can alleviate brain tissue injury after ischemia‐reperfusion, and has a protective effect on nervous system injury caused by cerebral ischemia‐reperfusion . Rhein is one of the effective components of rhubarb, and its protective effects on cerebrovascular conditions and tumors have been reported .…”

Section: Resultsmentioning

confidence: 99%

“…26 Rhubarb has effects against cerebral ischemia injury, can alleviate brain tissue injury after ischemia-reperfusion, and has a protective effect on nervous system injury caused by cerebral ischemia-reperfusion. 27,28 Rhein is one of the effective components of rhubarb, and its protective effects on cerebrovascular conditions and tumors have been reported. 29,30 RHL is a new compound produced by the reaction of lysine and rhein, which can dissolve in water and is convenient for drug administration.…”

Section: Discussionmentioning

confidence: 99%

Rhein lysinate improves motor function in rats with spinal cord injury via inhibiting p38 MAPK pathway

Hao

Wang

Daiping

et al. 2019

The Kaohsiung J of Med Scie

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The current study aims to investigate the effect of rhein lysinate (RHL) on neuromotor function in rats with spinal cord injury (SCI) and to explore the underlying mechanism. A total of 63 adult male SD rats were randomly divided into the sham group, SCI group and RHL group (SCI‐RHL group), with 21 rats in each group. Basso Beattie Bresnahan (BBB) scoring and the inclined plate test were used to evaluate the changes in motor function after SCI. Then, superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), and malondialdehyde (MDA) contents were quantified. Caspase‐3‐positive cells in spinal cord tissue were detected by immunohistochemical (IHC) staining, and protein expression was detected by Western blots. Here, our data showed that compared with the SCI group, the BBB score and inclined plate test score of the SCI‐RHL group were significantly higher than those of the SCI group 7 days after the RHL intervention. After 7 days of RHL treatment, the activities of SOD and GSH‐Px in the spinal cord increased significantly. At the same time, RHL reduced the MDA content in spinal cord tissue of SCI rats. Moreover, cleaved‐caspase‐3‐positive cells and apoptotic cells were significantly lower in the SCI‐RHL group than in the SCI group. More importantly, p38 mitogen‐activated protein kinase (p38 MAPK) was significantly decreased in the SCI‐RHL group compared with the SCI group. In summary, RHL can inhibit the activation of the p38 MAPK pathway after SCI, thereby reducing the apoptosis of spinal cord neurons and improving the neuromotor function of SCI rats.

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Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs

Cited by 71 publications

References 45 publications

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets

Rhein lysinate improves motor function in rats with spinal cord injury via inhibiting p38 MAPK pathway

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