Rhamnolipid increases H2S generation from waste activated sludge anaerobic fermentation: An overlooked concern

Fu, Qizi; Liu, Xuran; He, Dandan; Li, Xuemei; Li, Chenxi; Du, Mingting; Wang, Yan; Long, Sha; Wang, Dongbo

doi:10.1016/j.watres.2022.118742

Cited by 36 publications

(15 citation statements)

References 45 publications

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“…TS, VS, VFAs, carbohydrates, and proteins were estimated by the methods reported previously. − Methane and hydrogen were measured using a gas chromatograph (GC112A, China) based on the published literature studies . The membrane fluidity of microbial cells was analyzed via flow cytometry, with the analysis process being detailed in our previous studies. , An analysis of variance with least significant difference test was applied to evaluate the significance of results, and p < 0.05 was treated as statistically significant.…”

Section: Methodsmentioning

confidence: 99%

1-Butyl-3-methylimidazolium Chloride Affects Anaerobic Digestion through Altering Organics Transformation, Cell Viability, and Microbial Community

Liu

et al. 2023

Environ. Sci. Technol.

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1-Butyl-3-methylimidazolium chloride (BmimCl), an imidazolium-based ionic liquid, is considered the representative emerging persistent aquatic pollutant, and its environmental toxicity has attracted a growing concern. However, most of the investigations focused on monocultures or a single organism, with little information available on the complex syntrophic consortium that dominates the complex and successional biochemical processes, such as anaerobic digestion. In this study, the effect of BmimCl at environmentally relevant levels on glucose anaerobic digestion was therefore investigated in several laboratory-scale mesophilic anaerobic digesters to provide such support. Experimental results showed that BmimCl at 1–20 mg/L inhibited the methane production rate by 3.50–31.03%, and 20 mg/L BmimCl inhibited butyrate, hydrogen, and acetate biotransformation by 14.29%, 36.36%, and 11.57%, respectively. Toxicological mechanism studies revealed that extracellular polymeric substances (EPSs) adsorbed and accumulated BmimCl through carboxyl, amino, and hydroxyl groups, which destroyed the EPSs’ conformational structure, thereby leading to the inactivation of microbial cells. MiSeq sequencing data indicated that the abundance of Clostridium_sensu_stricto_1, Bacteroides, and Methanothrix decreased by 6.01%, 7.02%, and 18.45%, respectively, in response to 20 mg/L BmimCl. Molecular ecological network analysis showed that compared with the control, the lower network complexity, fewer keystone taxa, and fewer associations among microbial taxa were found in the BmimCl-present digester, indicating the reduced stability of the microbial community.

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Section: Methodsmentioning

confidence: 99%

1-Butyl-3-methylimidazolium Chloride Affects Anaerobic Digestion through Altering Organics Transformation, Cell Viability, and Microbial Community

Liu

et al. 2023

Environ. Sci. Technol.

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“…Sequencing data have been deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database (accession number: PRJNA889231). The abundances of the functional enzymes involved in the fermentative process based on the KEGG database were obtained through PICRUSt2 …”

Section: Methodsmentioning

confidence: 99%

“…The abundances of the functional enzymes involved in the fermentative process based on the KEGG database were obtained through PICRUSt2. 27 Other Analytical Methods. Soluble polysaccharides and proteins were determined by the Anthrone method 28 and Lowry method.…”

Section: Effects Of Pi Pretreatment On Eachmentioning

confidence: 99%

Novel Insights into the Mechanisms of Periodate-Based Pretreatment in Enhancing Short-Chain Fatty Acids from Waste Activated Sludge

et al. 2023

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Resource recovery in the form of short-chain fatty acids (SCFAs) from waste activated sludge (WAS) is usually limited due to the quantity and quality of SCFAs. To mitigate these restrictions, this study provides a novel method by using periodate (PI) pretreatment. With an increase in the PI concentration from 0 to 50 mg/g total solids (TS), the SCFA yield increased from 1486 to 3109 mg COD/L and acetic acid production was enhanced by more than twice. Further increase of PI had an adverse effect on SCFA production. Electron spin resonance analysis confirmed that hydroxyl radicals (•OH) and superoxide radicals (•O2 –) might play crucial roles in the pretreatment process. Further mechanism studies revealed that PI pretreatment improved the disintegration of WAS and decomposition of humus as well as lignocellulose, thereby providing more substrates for SCFA production. The model-based synthetic wastewater tests combined with enzymatic analyses revealed that the stronger suppression of SCFA consumers compared to SCFA generators causes SCFA accumulation; specifically, the inhibitory order was methanogenesis > hydrolysis > acetogenesis > acidogenesis. The suppressed gene expression of acetate-dependent (such as cdh [EC: 2.1.1.245]) and CO2-type methanogenesis (such as fwd [EC: 2.3.1.101] and mch [EC: 1.5.98.1]) further triggered SCFA accumulation with PI addition. Moreover, PI pretreatment caused the microbial community to shift toward the favorable hydrolytic and acidification-associated (e.g., Longilinea, Acinetobacter, and Macellibacteroides) microorganisms. This study is expected to broaden an unknown application of PI and provide an alternative strategy for resource recovery in some specific circumstances.

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“…26 At the phylum level, Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria were the dominant bacterium in the reactors with or without capsaicin, which are closely associated with the degradation of polysaccharides and protein and the production of VFAs. 87,88 At the genus level, the relative abundance values of Dysgonomonadacea, Petrimonas, and Macellibacteroides, which are responsible for the degradation of various polysaccharides and proteins, decreased from 6.5%, 5.3%, and 7.9% to 3.2%, 2.4%, and 1.2%, respectively, in the presence of capsaicin. 89 The addition of capsaicin also increased the total abundance of contaminantdegrading microbes such as Fonticella, Pirellulaceae, Enterococcus, and Herbinix, from 20.7% to 42.4%.…”

Section: Responses Of the Microbial Community To Food Additives In Fo...mentioning

confidence: 99%

Insights into the Occurrence, Fate, Impacts, and Control of Food Additives in Food Waste Anaerobic Digestion: A Review

Long

Liu

et al. 2023

Environ. Sci. Technol.

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The recovery of biomass energy from food waste through anaerobic digestion as an alternative to fossil energy is of great significance for the development of environmental sustainability and the circular economy. However, a substantial number of food additives (e.g., salt, allicin, capsaicin, allyl isothiocyanate, monosodium glutamate, and nonnutritive sweeteners) are present in food waste, and their interactions with anaerobic digestion might affect energy recovery, which is typically overlooked. This work describes the current understanding of the occurrence and fate of food additives in anaerobic digestion of food waste. The biotransformation pathways of food additives during anaerobic digestion are well discussed. In addition, important discoveries in the effects and underlying mechanisms of food additives on anaerobic digestion are reviewed. The results showed that most of the food additives had negative effects on anaerobic digestion by deactivating functional enzymes, thus inhibiting methane production. By reviewing the response of microbial communities to food additives, we can further improve our understanding of the impact of food additives on anaerobic digestion. Intriguingly, the possibility that food additives may promote the spread of antibiotic resistance genes, and thus threaten ecology and public health, is highlighted. Furthermore, strategies for mitigating the effects of food additives on anaerobic digestion are outlined in terms of optimal operation conditions, effectiveness, and reaction mechanisms, among which chemical methods have been widely used and are effective in promoting the degradation of food additives and increasing methane production. This review aims to advance our understanding of the fate and impact of food additives in anaerobic digestion and to spark novel research ideas for optimizing anaerobic digestion of organic solid waste.

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Rhamnolipid increases H2S generation from waste activated sludge anaerobic fermentation: An overlooked concern

Cited by 36 publications

References 45 publications

1-Butyl-3-methylimidazolium Chloride Affects Anaerobic Digestion through Altering Organics Transformation, Cell Viability, and Microbial Community

1-Butyl-3-methylimidazolium Chloride Affects Anaerobic Digestion through Altering Organics Transformation, Cell Viability, and Microbial Community

Novel Insights into the Mechanisms of Periodate-Based Pretreatment in Enhancing Short-Chain Fatty Acids from Waste Activated Sludge

Insights into the Occurrence, Fate, Impacts, and Control of Food Additives in Food Waste Anaerobic Digestion: A Review

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