Rhamnolipid biosurfactants: evolutionary implications, applications and future prospects from untapped marine resource

Kiran, George Seghal; Ninawe, A S; Lipton, Anuj Nishanth; Pandian, Vijayalakshmi; Selvin, Joseph

doi:10.3109/07388551.2014.979758

Cited by 32 publications

(22 citation statements)

References 127 publications

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“…Surface-active substances contribute to the emergent properties of biofilms. It is well known that rhamnolipids facilitate movement of P. aeruginosa in biofilms [85], but they are distributed among diverse microbial genera [86]. Surfactin, formed by B. subtilis, inhibits biofilm formation of different strains, including E. coli , P. mirabilis and S. enterica [87].…”

Section: Physical Properties Of Epsmentioning

confidence: 99%

EPS—Then and Now

2016

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“Slime” played a brief and spectacular role in the 19th century founded by the theory of primordial slime by Ernst Haeckel. However, that substance was never found and eventually abandoned. Further scientific attention slowly began in the 1930s referring to slime as a microbial product and then was inspired by “How bacteria stick” by Costerton et al. in 1978, and the matrix material was considered to be polysaccharides. Later, it turned out that proteins, nucleic acids and lipids were major other constituents of the extracellular polymeric substances (EPS), an acronym which was highly discussed. The role of the EPS matrix turns out to be fundamental for biofilms, in terms of keeping cells in proximity and allowing for extended interaction, resource capture, mechanical strength and other properties, which emerge from the life of biofilm organisms, including enhanced tolerance to antimicrobials and other stress. The EPS components are extremely complex and dynamic and fulfil many functional roles, turning biofilms into the most ubiquitous and successful form of life on Earth.

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Section: Physical Properties Of Epsmentioning

confidence: 99%

EPS—Then and Now

2016

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“…Several biosurfactants exhibit antibacterial, antifungal, and antiviral activities, which make them relevant molecules for applications in combating many diseases and infections . One of the preliminary research performed by Kiran et al , substantiate that stored grain pest Sitophilus oryzae can be controlled using glycolipid biosurfactant and this finding increases the scope of green and safe methods to protect stored produce from invading pests. Vatsa et al presented a review about the antimicrobial properties of rhamnolipids and the involvement of these molecules in the stimulation of immunity in plants.…”

Section: Introductionmentioning

confidence: 99%

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Goswami

Borah

Lahkar

et al. 2015

J. Basic Microbiol.

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The rhamnolipid biosurfactant (RL-DS9) extracted from the bacterial strain Pseudomonas aeruginosa DS9 was evaluated for its antifungal activity against Colletotrichum falcatum that causes red rot in sugarcane. The surface tension (ST) reduction, biosurfactant production, and antifungal activity of biosurfactant against C. falcatum were investigated by using the medium with different carbon sources and it was found to be maximum in glucose. Moreover, highest reduction of ST and production of biosurfactant was achieved at 4.5% (w/v) concentration of glucose. The efficacy of RL-DS9 was compared with a commercially available rhamnolipid (RL-R95) using microtitre plate assay. Results showed that at 100 μg ml(-1) concentration RL-DS9 exhibited 86.6% inhibition against C. falcatum spore germination, and in the same concentration RL-R95 showed 83.3% inhibition. From liquid chromatography-mass spectrometry (LC-MS) analysis, it was revealed that only two similar congeners Rha-(C10 ) and Rha-Rha-(C10:1 ) were found to be in common among both the rhamnolipids. In the plant bioassay test, it was noted that red rot incidence was reduced on the sugarcane plants treated with RL-DS9. This is the first report that rhamnolipid biosurfactant produced by Pseudomonas aeruginosa DS9 could be able to control red rot disease of sugarcane caused due to the infection with the fungus Colletotrichum falcatum.

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“…Biosurfactants are an important class of microbially synthesised compounds that have been extensively researched due to their diverse biological properties and functions (Van Hamme et al 2006; Gudiña et al 2013; Kiran et al 2016). Moreover, owing to their low toxicity and biodegradable nature, they exhibit potential for various commercial applications as environmentally friendly alternatives to synthetic surfactants (Nitschke and Costa 2007).…”

Section: Introductionmentioning

confidence: 99%

Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates

et al. 2017

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The quantitative and qualitative effect of water immiscible and miscible carbon-rich substrates on the production of biosurfactants, surfactin and rhamnolipids, by Bacillus amyloliquefaciens ST34 and Pseudomonas aeruginosa ST5, respectively, was analysed. A small-scale high throughput 96 deep-well micro-culture method was utilised to cultivate the two strains in mineral salt medium (MSM) supplemented with the water miscible (glucose, glycerol, fructose and sucrose) and water immiscible carbon sources (diesel, kerosene and sunflower oil) under the same growth conditions. The biosurfactants produced by the two strains were isolated by acid precipitation followed by an organic solvent extraction. Ultra-performance liquid chromatography coupled to electrospray ionisation mass spectrometry was utilised to analyse yields and characterise the biosurfactant variants. For B. amyloliquefaciens ST34, maximum surfactin production was observed in the MSM supplemented with fructose (28 mg L−1). In addition, four surfactin analogues were produced by ST34 using the different substrates, however, the C13–C15 surfactins were dominant in all extracts. For P. aeruginosa ST5, maximum rhamnolipid production was observed in the MSM supplemented with glucose (307 mg L−1). In addition, six rhamnolipid congeners were produced by ST5 using different substrates, however, Rha–Rha–C10–C10 and Rha–C10–C10 were the most abundant in all extracts. This study highlights that the carbon sources utilised influences the yield and analogues/congeners of surfactin and rhamnolipids produced by B. amyloliquefaciens and P. aeruginosa, respectively. Additionally, glucose and fructose were suitable substrates for rhamnolipid and surfactin, produced by P. aeruginosa ST5 and B. amyloliquefaciens ST34, which can be exploited for bioremediation or as antimicrobial agents.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0367-4) contains supplementary material, which is available to authorized users.

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Rhamnolipid biosurfactants: evolutionary implications, applications and future prospects from untapped marine resource

Cited by 32 publications

References 127 publications

EPS—Then and Now

EPS—Then and Now

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates

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