Rhamnolipid and surfactin: Anti-adhesion/antibiofilm and antimicrobial effects

Araújo, Lívia Vieira de; Guimarães, Carolina Reis; Marquita, Rafael Lucas da Silva; Santiago, Vânia Maria Junqueira; Souza, Maíra Paula de; Nitschke, Márcia; Freire, Denise Maria Guimarães

doi:10.1016/j.foodcont.2015.11.036

Cited by 117 publications

(66 citation statements)

References 46 publications

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“…Lipopeptides C3 were very effective against C albicans ; they reached nearly the maximum of biofilm formation inhibition (85%), at a very low concentration of about 0.03 mg/mL. Araujo et al reported that surfactin significantly reduced adhesion of Listeria monocytogenes ATCC 19112 on polystyrene surfaces with 54% inhibition, when used at a concentration of 0.50% ( w / v ).…”

Section: Resultsmentioning

confidence: 99%

“…This effect could be related to biosurfactants influence on the reduction of bacterial cell hydrophobic properties or on the repulsion between bacteria and abiotic surfaces . According to Araujo et al biofilm formation is inhibited by the conditioning of polystyrene and stainless steel 304 with rhamnolipids and surfactin biosurfactants, transforming the surfaces hydrophilic or less hydrophobic compared with the control. The decrease in surface hydrophobicity as a result of conditioning by biosurfactants entails a decrease in hydrophobic interactions with cell wall of microorganisms, and as a result, adhesion/biofilm formation is reduced.…”

Section: Resultsmentioning

confidence: 99%

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Isolation and characterization of kurstakin and surfactin isoforms produced by Enterobacter cloacae C3 strain

et al. 2018

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In this work, the extraction, structural analysis, and identification as well as antimicrobial, anti‐adhesive, and antibiofilm activities of lipopeptides produced by Enterobacter cloacae C3 strain were studied. A combination of chromatographic and spectroscopic techniques offers opportunities for a better characterization of the biosurfactant structure. Thin layer chromatography (TLC) and HPLC for amino acid composition determination are used. Efficient spectroscopic techniques have been utilized for investigations on the biochemical structure of biosurfactants, such as Fourier transform infrared (FT‐IR) spectroscopy and mass spectrometry analysis. This is the first work describing the production of different isoforms belonging to kurstakin and surfactin families by E cloacae strain. Three kurstakin homologues differing by the fatty acid chain length from C10 to C12 were detected. The spectrum of lipopeptides belonging to surfactin family contains various isoforms differing by the fatty acid chain length as well as the amino acids at positions four and seven. Lipopeptide C3 extract exhibited important antibacterial activity against Gram‐positive and Gram‐negative bacteria, antifungal activity, and interesting anti‐adhesive and disruptive properties against biofilm formation by human pathogenic bacterial strains: Salmonella typhimurium, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus cereus, and Candida albicans.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of kurstakin and surfactin isoforms produced by Enterobacter cloacae C3 strain

et al. 2018

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“…Surfactants are used to reduce the tensions between two insoluble surfaces such as between liquids and solids/gases and enhance the solubility of insoluble substrates in aqueous solutions (Benincasa et al, 2004;Cameotra and Makkar, 2010;Araujo et al, 2016). Use of biosurfactants instead of synthetic surfactants can significantly reduce the manufacturing costs by using lowcost substrates and efficient microorganisms to produce biosurfactants (Deleu and Paquot, 2004;Rufino et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Lima¹,

Andrade²,

RodrÃguez³

et al. 2017

Afr. J. Microbiol. Res.

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The world market for biosurfactants has grown gradually. However, the lack of competitiveness with chemical surfactants due to high cost of production remains a concern. Considering the need to reduce the costs of production, the aim of this work was to study the production and structural characterization of a biosurfactant produced by a strain of yeast Candida glabrata UCP 1556. The lowcost medium containing agro-industrial wastes whey 40% (v/v) and 20% (v/v) corn steep liquor were used as substrates in submerged fermentation. Biosurfactant production was detected by surface tension, oil displacement test and Critical Micelle Concentration (CMC). The structural characterization was performed by Fourier transform infrared (FT-IR), gas chromatography-mass spectrometry (GC-MS) and ionic profile. The stability of emulsions and potential in reducing the viscosity also were investigated. The results showed that the biosurfactant reduced the surface tension to 28.8 mN/m with CMC of 2% and showed anionic profile. Additionally, the biosurfactant formed stable emulsions at temperature (0 to 120°C), pH (2 to 12) and NaCl (2 to 12%), reduced the viscosity of soybean oil (

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“…The antimicrobial properties of rhamnolipid have often been reported (Gomaa, 2013;Liu et al, 2017;Santos et al, 2016;Varjani & Upasani, 2017). Very recently, defined rhamnolipids have shown good inhibition behavior against either photogenic bacteria (e.g., K. pneumonia, L. monocytogenes, P. aeruginosa, and S. aureus) (de Araujo et al, 2016;Haba et al, 2014) or phytopathogenic fungal species (e.g., C. albicans, Mucor miehei, Neurospora crassa, and Yarrowia lipolytica) (Sharma, Kalita, & Duarah, 2017;Silva et al, 2017).…”

Section: Antimicrobial Activity Of the Sacmentioning

confidence: 99%

Using Corn Husk Powder as a Novel Substrate to Produce a Surface Active Compound from Labrenzia aggregate KP‐5

Saimmai

Maneerat

Chooklin

2018

J Surfact & Detergents

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In this study, surface active compound (SAC)‐producing bacterial isolates were evaluated for SAC production using corn husk powder (CHP) as a sole carbon source. From the 51 isolates screened, Labrenzia aggregate KP‐5 produced the highest SAC activity. The highest SAC production (3.51 g L−1) was obtained when the strain was cultivated in a minimal salt medium containing 40 g L−1 CHP and 1 g L−1 commercial monosodium glutamate at 30 °C and 150 rpm after 51 h of cultivation. The produced SAC had the ability to decrease the surface tension of water from 72.0 to 25.5 mN m−1, with the critical micelle concentration of 9 mg L−1 (11.07 mM) and exhibited the highest emulsification activity (EA) of 81% against motor oil. The SAC showed stability at 4–121 °C and pH 4–10 against the surface and EA of vegetable oils and hydrocarbons, and showed tolerance at high salt concentrations (1–10% NaCl). The chemical structure of the SAC was confirmed as a rhamnolipid using Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and mass spectrometric analysis. The SAC did not exhibit inhibitory effects on various vegetables tested; however, strong inhibitory activity against Gram‐positive and Gram‐negative bacteria was observed. The application of SAC for microbial enhanced oil recovery by sand saturated with used lubricating oil resulted in above 89% of oil removal. The properties of the SAC we obtained from CHP have potential applications especially for microbial enhanced oil recovery and/or reducing the intensity of environmental contamination. In addition, the obtained SAC is a suitable alternative to antimicrobial agents.

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Rhamnolipid and surfactin: Anti-adhesion/antibiofilm and antimicrobial effects

Cited by 117 publications

References 46 publications

Isolation and characterization of kurstakin and surfactin isoforms produced by Enterobacter cloacae C3 strain

Isolation and characterization of kurstakin and surfactin isoforms produced by Enterobacter cloacae C3 strain

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Using Corn Husk Powder as a Novel Substrate to Produce a Surface Active Compound from Labrenzia aggregate KP‐5

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