Rhamnetin induces apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway

Abstract

The present study aimed to investigate whether rhamnetin induced apoptosis in human breast cancer cells and the underlying molecular mechanism of this anti cancer effect. The treatment of MCF-7 cells with rhamnetin was able to significantly inhibit cell proliferation and induce caspase-3/9 activity in a dose-and time-dependent manner, compared with untreated cells. In addition, treatment with rhamnetin was able to significantly promote the expression of p53 protein and microRNA (miR-)34a compared with untreated cells. The treatment with rhamnetin also suppressed the expression of Notch1 protein in MCF-7 cells compared with untreated cells. Subsequently, miR-24a expression was promoted in rhamnetin-treated MCF-7 cells using a miR-34a plasmid. The overexpression of miR-34a was able to significantly inhibit cell viability and induce caspase-3/9 activity in MCF-7 cells following treatment with rhamnetin. Furthermore, the overexpression of miR-34a was able to significantly promote the expression of p53 protein and miR-34a, and suppress the expression of Notch1 protein in rhamnetin-treated MCF-7 cells. Therefore, the results of the present study demonstrated that rhamnetin induced apoptosis in human breast cancer cells via the miR-34a/Notch-1 signaling pathway.

Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells

“…Meanwhile, OC contains a high amount of chlorophyll (Park & Park, 1998), and chlorophyll can be degraded into grey‐brown compounds such as pheophorbide and pheophytin (Heaton & Marangoni, 1996). In addition, a recent study revealed that rhamnetin can induce apoptosis in human breast cancer cells (Lan et al., 2019). Moreover, isorhamnetin was reported to exhibit anti‐proliferative and pro‐apoptotic effects in AGS human gastric cancer cells (Ramachandran et al., 2012), and Somerset and Johannot (2008) reported that pear is the main source of isorhamnetin in adult Australian population.…”

Notch and breast cancer metastasis: Current knowledge, new sights and targeted therapy (Review)

“…miR-34a has been demonstrated to function as an important tumor suppressor by regulating a variety of tumor progression steps, including cell proliferation, invasion and apoptosis, and Notch, which is a target gene of miR-34a (142). In metastatic breast cancer cells, overexpression of miR-34a significantly increases the protein level of tumor suppressor gene p53 and decreases the expression of Notch1, thereby inhibiting cell proliferation, invasion and inducing apoptosis (143,144). Additionally, miR-34a can sensitize metastatic breast cancer cells to paclitaxel and adriamycin (chemotherapeutic drugs for breast cancer) partly by downregulating Notch1 expression (142,145).…”

The effect of quercetin on microRNA expression: A critical review

“…Scientists are interested in derivatives of quercetin such as rhamnetin that modulates miR-34a in different cell lines [87][88][89] . Finally, we found an article, which reversed the usual logic.…”