RGD-Modified Titanium as an Improved Osteoinductive Biomaterial for Use in Dental and Orthopedic Implants

Abstract

This study describes the synthesis, surface analysis, and biological evaluation of bioactive titanium surfaces. The aim was to achieve an improved effect on osteoinduction in dental and orthopedic implants. For this purpose, a chemistry was developed, which allows to bind the bioactive cyclopeptide cRGDfK covalently to biomedically used titanium via polyethylene glycol linkers of different lengths. The chemical process is practicable, robust, and metal-free. The resulting chemically modified titanium plates show improved osteoinductive properties. The modification with cRGDfK targets the integrin α v β 3 , which is highly expressed in osteoblasts and is essential for many basic functions in the development of bone tissue. The successful immobilization of cRGDfK on titanium surfaces has been demonstrated by contact angle measurements and X-ray photoelectron spectroscopy. We show in in vitro studies that the presence of the cRGDfK peptide on titanium surfaces has a positive effect on bone formation.

A drug delivery system constructed by a fusion peptide capturing exosomes targets to titanium implants accurately resulting the enhancement of osseointegration peri-implant

“…When the complexes EXO-PEPs were constructed and incubated on the titanium surface, EXO-PEP1 adsorbed in the least quantity and the most connected was EXO-PEP3, suggesting PEP1 gained the low ability targeting to the titanium and PEP3 affinity as TBP3, which indicating PEP3 retained the fully titanium targeting of TBP motif and the exosomal anchoring of CP05 motif simultaneously [ 56 ]. The result of IVIS showed the similar result in vivo.…”

Recent Advances in Processing of Titanium and Titanium Alloys through Metal Injection Molding for Biomedical Applications: 2013–2022

“…Biomaterial implants are commonly used to replace and restore damaged or deteriorated organs or tissues in the human body [2]. This includes dental and orthopedic implants, ligaments, intraocular lenses, vascular grafts, artificial hearts, heart valves, biosensors, and cardiac pacemakers [5][6][7][8][9][10][11][12][13]. An implant should function flawlessly for a lifetime.…”

“Clickable” Polymer Brush Interfaces: Tailoring Monovalent to Multivalent Ligand Display for Protein Immobilization and Sensing

“…The past decade has witnessed the evolution of polymeric surface coatings from a simple protection barrier to a functional interface, which imparts functional attributes to the material through specific interactions and communication with its environment. In particular, polymeric coatings bearing bioactive ligands ranging from small molecules to biomacromolecules play a critical role in realizing various diagnostic and biosensing platforms. − For such applications, a polymeric coating that is stable in an aqueous environment, inherently anti-biofouling, and can be easily conjugated with biological probes is often desirable. In light of the demand for workability under aqueous conditions, as a general approach, hydrophilic polymers are chemically tethered onto the underlying inorganic substrate through either a “graft-to” or “graft-from” approach.…”