RGD-dependent entry of coxsackievirus A9 into host cells and its bypass after cleavage of VP1 protein by intestinal proteases

Roivainen, Merja; Hyypiä, Timo; Piirainen, Liisa; Kalkkinen, Nisse; Stanway, Glyn; Hovi, Tapani

doi:10.1128/jvi.65.9.4735-4740.1991

Cited by 138 publications

(60 citation statements)

References 25 publications

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“…This function was clearly impaired by infection with the isolate DM. Paananen et al coxsackievirus A9 [Roivainen et al, 1991] and other picornaviruses: foot-and-mouth disease virus [Berinstein et al, 1995;Neff et al, 1998] and human parechovirus 1 [Roivainen et al, 1991;Stanway et al, 1994;Joki-Korpela et al, 2001]. RGD-independent receptor binding to vitronectin has been reported for hantavirus [Gavrilovskaya et al, 1998].…”

Section: Discussionmentioning

confidence: 99%

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Molecular and biological analysis of echovirus 9 strain isolated from a diabetic child

Paananen

Ylipaasto

Rieder

et al. 2003

Journal of Medical Virology

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The full-length infectious cDNA clone was constructed and sequenced from the strain DM of echovirus 9, which was recently isolated from a 6-week-old child at the clinical onset of type 1 diabetes. Parallel with the isolate DM, the full-length infectious cDNA clone of the prototype strain echovirus 9 Barty (Barty-INF), was constructed and sequenced. Genetic relationships of the sequenced echo 9 viruses to the other members of the human enterovirus type B species were studied by phylogenetic analyses. Comparison of capsid protein sequences showed that the isolate DM was closely related to both prototype strains: Hill and Barty-INF. The only exception was the inner capsid protein VP4 where serotype specificity was not evident and the isolate DM clustered with the strain Hill and the strain Barty-INF with echovirus 30 Bastianni. Likewise, the nonstructural protein coding region, P2P3, of isolate DM was more similar to strain Hill than to strain Barty-INF. However, like echovirus 9 Barty, the isolate DM contained the RGD-motif in the carboxy terminus of capsid protein VP1. By blocking experiments using an RGD-containing peptide and a polyclonal rabbit antiserum to the alpha(v)beta(3)-integrin, it was shown that this molecule works as a cellular receptor for isolate DM. By using primary human islets, it was shown that the isolate DM is capable of infecting insulin-producing beta-cells like the corresponding prototype strains did. However, only isolate DM was clearly cytolytic for beta-cells. The infectious clones that were made allow further investigations of the molecular features responsible for the diabetogenicity of the isolate DM.

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Section: Discussionmentioning

confidence: 99%

“…The assay was carried out in confluent GMK cells in six well plates as described earlier [Roivainen et al, 1991]. Briefly, the cells were exposed either to RRRGDLpeptide (50 ml/well) at a 1 mM concentration or to polyclonal rabbit antiserum (50 ml/well) to the vitronectin receptor, a v b 3 , at a dilution of 1:50.…”

Section: Plaque Protection Assaymentioning

confidence: 99%

Molecular and biological analysis of echovirus 9 strain isolated from a diabetic child

Paananen

Ylipaasto

Rieder

et al. 2003

Journal of Medical Virology

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“…Integrin v 3 (Roivainen et al, 1991;Roivainen et al, 1994;Berinstein et al, 1995;Triantafilou et al, 1999); GRP78 (Triantafilou et al, 2002); MHC class I via 2 -microglobulin (Triantafilou et al, 1999) Coxsackievirus B1-6 CAR (Bergelson et al, 1997;Shafren et al, 1997c;Tomko et al, 1997;Martino et al, 2000;He et al, 2001); murine CAR (Tomko et al, 1997;Bergelson et al, 1998) Coxsackievirus B1, B3, B5 DAF Shafren et al, 1995;Martino et al, 1998); integrin v 6 (Agrez et al,…”

Section: Coxsackievirus A9mentioning

confidence: 99%

Evolution of Cell Recognition by Viruses: A Source of Biological Novelty with Medical Implications

Baranowski

Ruiz-Jarabo

Pariente

et al. 2003

Advances in Virus Research

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Receptor classCellular structure a Extracellular matrix components, sugar derivatives and lipids GalactosylceramideGangliosides Glycosaminoglycans (heparan and chondroitin sulfates) Phospholipids Sialic acid (N-acetylneuraminic acid, N-acetyl-9-O-acetylneuraminic acid and N-glycolylneuraminic acid 3-O-sulfated heparan sulfate Cell adhesion and cell-cell contact proteins -Dystroglycan Coxsackievirus-adenovirus receptor (CAR; Ig superfamily) CD4 (Ig superfamily) CEACAMs (including Bgp1a, Bgp2, and pregnancy-specific glycoprotein) Intercellular adhesion molecule type 1 (ICAM-1; Ig superfamily) Integrins Junction adhesion molecule (JAM) Laminin receptor (high affinity) MHC class I and 2 -microglobulin Neural cell adhesion molecule (NCAM) Signaling lymphocyte activation molecule

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“…Titres for infectious virus in the heart and plasma samples were detected using the plaque-forming assay as previously described [18]. Myocardial tissue sections of each animal were stained with haematoxylin-eosin for histopathological analysis.…”

Section: Experimental Myocarditis Virus Titration and Histopathologymentioning

confidence: 99%

Glutathione depletion and cardiomyocyte apoptosis in viral myocarditis

Kytö

Lapatto

Lakkisto

et al. 2004

Eur J Clin Investigation

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RGD-dependent entry of coxsackievirus A9 into host cells and its bypass after cleavage of VP1 protein by intestinal proteases

Abstract: The recently reported nucleotide sequence of coxsackievirus A9 (CAV-9) showed that unlike other enteroviruses, CAV-9 has an insertion of about 17 amino acids at the C-terminal end of VP1 (K. H. Chang, P.

Cited by 138 publications

References 25 publications

Molecular and biological analysis of echovirus 9 strain isolated from a diabetic child

Molecular and biological analysis of echovirus 9 strain isolated from a diabetic child

Evolution of Cell Recognition by Viruses: A Source of Biological Novelty with Medical Implications

Glutathione depletion and cardiomyocyte apoptosis in viral myocarditis

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