RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls

Klaassen, Michiel T.; Trindade, Luisa M.

doi:10.1016/j.carbpol.2019.115353

Cited by 29 publications

(10 citation statements)

References 56 publications

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“…Along with xyloglucans, galactan domains of RG I interact with cellulose microfibrils, hence maintaining cell wall structure ( Zykwinska et al, 2007 ). A truncation of galactan side chains also interferes with porosity and thus water-binding capability of the plant cell walls ( Klaassen and Trindade, 2020 ). However, a gals1 gals2 gals3 triple mutant did not display a macroscopic phenotype ( Ebert et al, 2018 ), suggesting that the severe reduction of 4-Gal does not account for the impaired growth of Mn 2+ -deficient bicat3 mutants.…”

Section: Discussionmentioning

confidence: 99%

The trans-Golgi-localized protein BICAT3 regulates manganese allocation and matrix polysaccharide biosynthesis

Yang

Hause

et al. 2022

Plant Physiology

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Manganese (Mn2+) is essential for a diversity of processes, including photosynthetic water splitting and the transfer of glycosyl moieties. Various Golgi-localized glycosyltransferases that mediate cell wall matrix polysaccharide biosynthesis are Mn2+-dependent, but the supply of these enzymes with Mn2+ is not well understood. Here, we show that the BIVALENT CATION TRANSPORTER 3 (BICAT3) localizes specifically to trans-cisternae of the Golgi. In agreement with a role in Mn2+ and Ca2+ homeostasis, BICAT3 rescued yeast (Saccharomyces cerevisiae) mutants defective in their translocation. Arabidopsis (Arabidopsis thaliana) knockout mutants of BICAT3 were sensitive to low Mn2+ and high Ca2+ availability and showed altered accumulation of these cations. Despite reduced cell expansion and leaf size in Mn2+-deficient bicat3 mutants, their photosynthesis was improved, accompanied by an increased Mn content of chloroplasts. Growth defects of bicat3 corresponded with an impaired glycosidic composition of matrix polysaccharides synthesized in the trans-Golgi. In addition to the vegetative growth defects, pollen tube growth of bicat3 was heterogeneously aberrant. This was associated with a severely reduced and similarly heterogeneous pectin deposition and caused diminished seed set and silique length. Double mutant analyses demonstrated that the physiological relevance of BICAT3 is distinct from that of ER-TYPE CA2+-ATPASE 3 (ECA3), a Golgi-localized Mn2+/Ca2+-ATPase. Collectively, BICAT3 is a principal Mn2+ transporter in the trans-Golgi whose activity is critical for specific glycosylation reactions in this organelle and for the allocation of Mn2+ between Golgi apparatus and chloroplasts.

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Section: Discussionmentioning

confidence: 99%

The trans-Golgi-localized protein BICAT3 regulates manganese allocation and matrix polysaccharide biosynthesis

Yang

Hause

et al. 2022

Plant Physiology

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“…2013 ). Whereas the accumulation of truncated galactan side chains due to over expression of β-galactosidase in potato ( Solanum tuberosum L.) causes reduction in the water-binding capacity and a decrease in the tolerance of tubers to dehydration ( Klaassen and Trindade 2020 ).…”

Section: Molecular Implications Of In Muro Modifications For the Control Of Freezing Behaviormentioning

confidence: 99%

Responses of the Plant Cell Wall to Sub-Zero Temperatures: A Brief Update

Takahashi

Willick

Kasuga

et al. 2021

Plant and Cell Physiology

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Our general understanding of plant responses to sub-zero temperatures focuses on mechanisms that mitigate stress to the plasma membrane. The plant cell wall receives comparatively less attention and questions surrounding its role in mitigating freezing injury remains unresolved. Despite recent molecular discoveries that provide insight into acclimation responses, the goal of reducing freezing injury in herbaceous and woody crops remains elusive. This is likely due to the complexity associated with adaptations to low temperatures. Understanding how leaf cell walls of herbaceous annuals promote tissue tolerance to ice does not necessarily lead to understanding how meristematic tissues are protected from freezing by tissue-level barriers formed by cell walls in overwintering tree buds. In this mini-review, we provide an overview of biological ice nucleation and explain how plants control the spatiotemporal location of ice formation. We discuss how sugars and pectin side chains alleviate adhesive injury that develops at sub-zero temperatures between the matrix polysaccharides and ice. The importance of site-specific cell wall elasticity to promote tissue expansion for ice accommodation and control of porosity to impede ice growth and promote supercooling will be presented. How specific cold-induced proteins modify plant cell walls to mitigate freezing injury will also be discussed. The opinions presented in this report emphasize the importance of a plant’s developmental physiology when characterizing mechanisms of freezing survival.

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“…Evidence suggests that walls rich in galactan are firm and stiff, whereas the abundance of arabinan provides wall elasticity [98][99][100][101]. Moreover, these two forms of RG-I side chains are believed to display a variable occurrence within the cell walls [102,103] and can be modified depending on different biotic and abiotic factors [65,[104][105][106][107]. The presented research showed that the LM5 epitope was not detected in the tissues of the DZ in the control roots, but that it occurred in the walls of different tissues in the roots that were grown in (+) AuNP and (−) AuNP solutions.…”

Section: Aunp Affect the Presence And Distribution Of Pectic Epitopes In Barley Rootsmentioning

confidence: 99%

Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots

Milewska‐Hendel

Sala

Gepfert

et al. 2021

Cells

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The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment. In such conditions, plants come into direct contact with NP. Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient. Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP). The analyses were performed using the immunohistological method and fluorescence microscopy. The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.

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RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls

Cited by 29 publications

References 56 publications

The trans-Golgi-localized protein BICAT3 regulates manganese allocation and matrix polysaccharide biosynthesis

The trans-Golgi-localized protein BICAT3 regulates manganese allocation and matrix polysaccharide biosynthesis

Responses of the Plant Cell Wall to Sub-Zero Temperatures: A Brief Update

Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots

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