Rfm1, a Novel Tethering Factor Required To Recruit the Hst1 Histone Deacetylase for Repression of Middle Sporulation Genes

McCord, Ron; Pierce, Michael; Xie, Junhua; Wonkatal, Sandeep; Mickel, Carolyn; Vershon, Andrew K.

doi:10.1128/mcb.23.6.2009-2016.2003

Cited by 72 publications

(121 citation statements)

References 27 publications

Supporting

Mentioning

115

Contrasting

Order By: Relevance

“…In addition, we detected genes encoding metabolic functions (BNA2, CAT2, OPI3, SNO2, and SNZ2), a heatshock protein (HSP26), and NDT80, which encodes a sporulation-specific transcription factor required for middle-meiotic gene expression and activation of its own expression via a positive feedback loop (Pak and Segall 2002). In this context, it is noteworthy that three of the upregulated genes (NDT80, BNA2, and YJR079W) were previously reported to be repressed by Sum1, a DNAbinding transcription factor that represses Ndt80 and a subset of Ndt80-induced genes during vegetative growth (Hepworth et al 1998;Xie et al 1999;McCord et al 2003;Jolly et al 2005). However, the SUM1 transcript itself is not altered under these conditions, suggesting that the protein may be regulated by post-transcriptional mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Mitotic Expression of Spo13 Alters M-Phase Progression and Nucleolar Localization of Cdc14 in Budding Yeast

et al. 2010

View full text Add to dashboard Cite

Spo13 is a key meiosis-specific regulator required for centromere cohesion and coorientation, and for progression through two nuclear divisions. We previously reported that it causes a G2/M arrest and may delay the transition from late anaphase to G1, when overexpressed in mitosis. Yet its mechanism of action has remained elusive. Here we show that Spo13, which is phosphorylated and stabilized at G2/M in a Cdk/Clb-dependent manner, acts at two stages during mitotic cell division. Spo13 provokes a G2/M arrest that is reversible and largely independent of the Mad2 spindle checkpoint. Since mRNAs whose induction requires Cdc14 activation are reduced, we propose that its anaphase delay results from inhibition of Cdc14 function. Indeed, the Spo13-induced anaphase delay correlates with Cdc14 phosphatase retention in the nucleolus and with cyclin B accumulation, which both impede anaphase exit. At the onset of arrest, Spo13 is primarily associated with the nucleolus, where Cdc14 accumulates. Significantly, overexpression of separase (Esp1), which promotes G2/M and anaphase progression, suppresses Spo13 effects in mitosis, arguing that Spo13 acts upstream or parallel to Esp1. Given that Spo13 overexpression reduces Pds1 and cyclin B degradation, our findings are consistent with a role for Spo13 in regulating APC, which controls both G2/M and anaphase. Similar effects of Spo13 during meiotic MI may prevent cell cycle exit and initiation of DNA replication prior to MII, thereby ensuring two successive chromosome segregation events without an intervening S phase.

show abstract

Section: Resultsmentioning

confidence: 99%

Mitotic Expression of Spo13 Alters M-Phase Progression and Nucleolar Localization of Cdc14 in Budding Yeast

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Sum1 bound at the NDT80 promoter recruits the histone deacetylase Hst1, which leads to repression of NDT80 expression, even when Ume6 is converted to an activator by binding of Ime1 (Xie et al 1999). The Sum1-Hst1 complex is also bound to MSE elements at many other Ndt80-regulated genes where it is responsible for their repression in vegetative cells (McCord et al 2003).…”

Section: A Regulatory Cascade Controls the Events Of Sporulationmentioning

confidence: 99%

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

View full text Add to dashboard Cite

In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae. TABLE OF CONTENTS Abstract 737Introduction 738

show abstract

“…One such example is the Sum1 protein, which acts as a repressor of a subset of meiotic genes during the mitotic cell cycle (Xie et al 1999). In this function, Sum1 recruits the Sir2 homolog Hst1 to deacetylate histones in the promoter region of some, but not all of the Sum1-repressed genes (McCord et al 2003). Moreover, Sum1 and Hst1 both interact with the tethering factor Rfm1 (repression factor of MSE [middle sporulation element]), thus targeting Hst1 to a subset of Sum1-regulated genes (McCord et al 2003).…”

mentioning

confidence: 99%

Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression

Irlbacher¹,

Franke²,

Manke³

et al. 2005

Genes Dev.

View full text Add to dashboard Cite

Heterochromatinization at the silent mating-type loci HMR and HML in Saccharomyces cerevisiae is achieved by targeting the Sir complex to these regions via a set of anchor proteins that bind to the silencers. Here, we have identified a novel heterochromatin-targeting factor for HML, the protein Sum1, a repressor of meiotic genes during vegetative growth. Sum1 bound both in vitro and in vivo to HML via a functional element within the HML-E silencer, and sum1⌬ caused HML derepression. Significantly, Sum1 was also required for origin activity of HML-E, demonstrating a role of Sum1 in replication initiation. In a genome-wide search for Sum1-regulated origins, we identified a set of autonomous replicative sequences (ARS elements) that bound both the origin recognition complex and Sum1. Full initiation activity of these origins required Sum1, and their origin activity was decreased upon removal of the Sum1-binding site. Thus, Sum1 constitutes a novel global regulator of replication initiation in yeast.[Keywords: HML-D; Hst1; chromatin; replication; origin; ARS] Supplemental material is available at http://www.genesdev.org.

show abstract

Rfm1, a Novel Tethering Factor Required To Recruit the Hst1 Histone Deacetylase for Repression of Middle Sporulation Genes

Cited by 72 publications

References 27 publications

Mitotic Expression of Spo13 Alters M-Phase Progression and Nucleolar Localization of Cdc14 in Budding Yeast

Mitotic Expression of Spo13 Alters M-Phase Progression and Nucleolar Localization of Cdc14 in Budding Yeast

Sporulation in the Budding Yeast Saccharomyces cerevisiae

Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression

Contact Info

Product

Resources

About