RFLPs are detected by two probes isolated by chromosome walking from pTD3–21 (D15S10) which maps to 15q11.2–12

Abstract

Source/Description: A 1.7 kb DNA segment, subcloned in a modified pGEM3 vector (Promega Biotec) and derived from a X EMBL3 library, constructed from the somatic cell hybrid 20XP3542-1-4 (Stallings et al. 1988). SacII/PstI double digestion yields two inserts of 1.2 kb and 0.5 kb. The 1.2 insert is used for labeling. Polymorphism: MspI (CCGG) detects a two allele polymorphism with bands at either 1.3 kb (A1) or 0.8 kb and 0.5 kb (A2).