RFLP Markers for the Selection of Pollination-constant and Non-astringent (PCNA)-Type Persimmon and Examination of the Inheritance Mode of the Markers

Abstract

We investigated marker-assisted selection of the pollination constant and non-astringent (PCNA) trait and inheritance modes of the marker locus in persimmon (Diospyros kaki Thunb.) using restriction fragment length polymorphism (RFLP) analysis. In two backcross progenies, FU-170 and FU-275, 5 of 71 and 23 of 101 offspring, respectively, were judged as PCNA genotype by the marker phenotype. The astringency type of individual offspring was determined by measuring the size of tannin cells and soluble tannin content in mature fruit, thus confirming complete co-segregation of RFLP markers and the non-PCNA phenotype in 32 and 68 fruit-obtainable offspring from FU-170 and FU-275, respectively; therefore, RFLP markers can be used for PCNA genotype detection. Their segregation ratio deviated significantly from that of disomic inheritance. We also discuss the possibility of polysomic inheritance of RFLP markers.

Conversion of RFLP Markers for the Selection of Pollination-Constant and Non-Astringent Type Persimmons (Diospyros kaki Thunb.) into PCR-Based Markers

“…As in the case of the backcross progeny derived from 'Nishimura-wase', 2 RFLP markers, namely, A1 and A2, were segregated in the FU-275 progeny, which was derived from 'Aizumishirazu', and the accuracy of the selection using the RFLP markers has been confirmed (Kanzaki et al, 2008a). However, unlike the case in 'Nishimura-wase'-derived progeny, the primer pair E4/A2r did not produce any fragments in FU-275 (Fig.…”

“…In the previous report, we demonstrated that the AST locus should be regarded as a polysomic locus; however, it remains to be determined whether the inheritance mode of the locus is tetra-or hexasomic (Kanzaki et al, 2008a). In either case, it is necessary to consider that multiple alleles are located separately on 4 or 6 different homologous chromosomes at the single AST locus.…”

“…Because the genomic composition of the cultivated persimmon, which is mostly hexaploid, has not been clarified, it is difficult to fully elucidate the genetic nature of the gene controlling the PCNA trait through a segregation analysis of fruit phenotypes. However, our previous studies on molecular markers for the selection of PCNA-type offspring provided a clue to its genotypic make-up (Kanzaki et al, 2001(Kanzaki et al, , 2008a.…”

“…These results suggest that each RFLP marker is associated separately with the gene conferring the non-PCNA trait. Furthermore, segregation analysis of these RFLP markers indicated that both RFLP markers are linked to an identical locus, which we termed the AST locus, and that the mode of inheritance of the AST locus would be polysomic rather than disomic (Kanzaki et al, 2001(Kanzaki et al, , 2008a.…”

The Validity of Marker-assisted Selection Using DNA Markers Linked to a Pear Scab Resistance Gene (Vnk) in Two Populations

“…These DNA markers could improve selection efficiency in Japanese pear breeding programs. Recently, DNA makers linked to the desirable traits for breeding in other fruit trees also developed (Kanzaki et al, 2008;Nakano et al, 2008).…”